CA and Tz cooperative inhibition of ERBB2+ cell survival is transient. Blue lines represent cell survival of SKBR-3 and BT474 cells continuously cultured with CA + Tz (cultures #2). Red lines represent cell survival of SKBR-3 and BT474 cells cultured for up to 6d with CA + Tz and for further 8d with control medium (cultures #1). Cell survival is expressed as arbitrary units (A.U.) after exposure of cultures to Alamar Blue for 4 h. The arrow represents the time point at which the medium containing the two drugs was replaced with control medium in cultures #1. Mean values and standard deviation (indicated as vertical bars) from four independent replicates (n = 4) are shown. P < 0.001 (***) (TIFF 817 kb
