19 research outputs found
Triagem de macroalgas bĂȘnticas do litoral de Santa Catarina biomonitorada pelo ensaio de letalidade para larvas de Artemia salina e investigação fitoquĂmica de Pterocladiella capillacea
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de CiĂȘncias da SaĂșde. Programa de PĂłs-Graduação em FarmĂĄcia
Investigação quĂmica de espĂ©cies do gĂȘnero Laurencia Lamourox na costa sul brasileira
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de CiĂȘncias da SaĂșde, Programa de PĂłs-Graduação em FarmĂĄcia, FlorianĂłpolis, 2009O complexo Laurencia Lamouroux (Rhodophyceae) compreende quatro gĂȘneros com grande variabilidade morfolĂłgica que representam os maiores produtores de metabĂłlitos secundĂĄrios do ambiente marinho. As substĂąncias provenientes destas algas sĂŁo predominantemente sesquiterpenos, diterpenos e C15-acetogeninas que usualmente sĂŁo caracterizados pela presença de ĂĄtomos de halogĂȘnio em suas estruturas. Existem mais de 19 espĂ©cies catalogadas e na regiĂŁo sul observa-se a presença das espĂ©cies Laurencia obtusa, L. microcladia, L. majuscula, L. catarinensis e L. flagellifera. O estudo quĂmico do extrato orgĂąnico de Laurencia microcladia indicou a presença de um metabĂłlito majoritĂĄrio conhecido como elatol, encontrado em outras espĂ©cies de Laurencia Lamouroux. Estudos em cromatografia delgada demonstraram a presença dessa substĂąncia em extratos de organismos como ouriço do mar (Echinometra lucunter) e pepino do mar (Holothuria grisea). Foram realizados ensaios de herbivoria com ouriços do mar indicando que este composto apesar de apresentar atividade anti-herbivoria foi consumido pelos ouriços atĂ© a concentração de 0,2 mg/pellets. A investigação de atividades biolĂłgicas com o elatol obtido neste trabalho, indicou atividade tripanocida frente ao Trypanossoma cruzi (CI50 = 13,86 (12,39-15,51) ”M) e leishmanicida frente a Leishmania amazonensis (CI50 = 2,54 (2,44-2,64) ”M) e L. chagasi (CI50 = 2,56 (1,94-3,38) ”M), mas nĂŁo apresentou atividade antiviral frente aos vĂrus herpĂ©ticos do tipo 1 (2 cepas) [modelo viral de genoma DNA], e do rotavĂrus SA-11 [modelo viral de genoma RNA]. TambĂ©m apresentou atividade antitumoral in vitro e in vivo para cĂ©lulas B16F10 (melanoma). A investigação quĂmica do extrato orgĂąnico de Laurencia catarinsensis resultou no isolamento de cerca de 40 compostos puros, dentre os quais, 21 possuem caracterĂsticas estruturais semelhantes. Destes 21 compostos, treze compostos sĂŁo inĂ©ditos na literatura e identificados como: o-metilfurocaespitano e seu estereoisĂŽmero, o isĂŽmero do caespitano, o derivado 5-acetoxi do caespitol, o derivado 5-acetoxi do caespitano, o derivado 5-acetoxi do 8-acetilcaespitol, o derivado 5-hidroxi do 8-acetilcaespitol, o derivado 5-acetoxi do laucapiranĂłide A, o derivado 5-hidroxi laucapiranĂłide, o derivado do isocaespitol, dois derivados do caespitol e derivado do sniderol. Adicionalmente, os compostos caespitol e isocaespitol foram submetidos a avaliação antitumoral em cĂ©lulas T47D (tumor de mama), U373 (glioblastoma), A549 (cĂąncer de pulmĂŁo) e PC3 (cĂąncer de prĂłstata) verificando-se atividade nas trĂȘs Ășltimas linhagens. Os compostos caespitol e derivado 5-acetoxi do caespitol nĂŁo apresentaram atividade antiviral frente aos vĂrus herpĂ©ticos do tipo 1 (2 cepas) [modelo viral de genoma DNA], e do rotavĂrus SA-11 [modelo viral de genoma RNA]. O estudo quĂmico do extrato aquoso de Laurencia flagellifera indicou a presença de dois compostos majoritĂĄrios conhecidos como monogalactopiranosilglicerol e sulfoquinovosildiacilglicerol
Preliminary data of antioxidant activity of green seaweeds (Ulvophyceae) from the Southwestern Atlantic and Antarctic Maritime islands
Background. Seaweeds must survive in highly competitive environments and thus develop defense strategies that may produce highly diversified antioxidant compounds. Goals. The main objective of this work was to assess the antioxidant activity of green seaweeds. Methods. Six species of ulvophycean chlorophytes were collected during spring/ summer, between the Antarctic (Monostroma hariotii, Protomonostroma rosulatum and Ulva hookeriana formerly as U. bulbosa) and the southwestern Atlantic Ocean (Gayralia brasiliensis, Protomonostroma undulatum and Ulvafasciata). They were then tested for their antioxidant activities using the 2.2-diphenyl-picrylhydrazyl (DPPH) radical scavenging method and by quantification of their phenolic (expressed as gallic acid equivalent GAE and carotenoid contents. Results. Among the evaluated species, P. rosulatum and U. hookeriana showed high antioxidant potential (77.9±2.8 and 53.1±15.0%, respectively) and high phenolic content (176±6.0 and 144.7±8.9 ?g GAE g-1, respectively). These species were collected on King George Island (South Shetland archipelago, around the Antarctic Peninsula) and their higher antioxidant potential may be associated with adaptation to the high incidence of UV rays in this region during summer. In general, tested seaweeds, mainly the samples collected in the Antarctic and Chilean Patagonia, showed higher values of phenolic (from 58.3±2.0 to 144.7±8.9 ?g GAE g-1) and carotenoid contents (from 23.4±0.2 to 51.5±0.1 ?g ?-carotene g-1). Conclusions. The presence and levels of these compounds suggest that the target seaweeds may have high antioxidant potential. Also the antioxidant activity could be associated with the occurrence area of the species instead of the order or taxonomic group to which they belong
The 5 alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals
The aim of the present study was to determine the expression of the genes for type 1 (SDR5A1) and type 2 (SDR5A2) 5α-reductase isoenzymes in scalp hairs plucked from 33 hirsute patients (20 with polycystic ovary syndrome and 13 with idiopathic hirsutism) and compare it with that of 10 men and 15 normal women. SDR5A1 and SDR5A2 expression was estimated by RT-PCR using the gene of the ubiquitously expressed protein Ă2-microglobulin as an internal control. The results are expressed as arbitrary units in relation to Ă2- microglobulin absorbance (mean ± SEM). SDR5A2 expression was not detected in any hair samples analyzed in this study. No differences were found in SDR5A1 mRNA levels between men and normal women (0.78 ± 0.05 vs 0.74 ± 0.06, respectively). SDR5A1 gene expression in the cells of hair plucked from the scalp of normal women (0.85 ± 0.04) and of women with polycystic ovary syndrome (0.78 ± 0.05) and idiopathic hirsutism (0.80 ± 0.06) was also similar. These results indicate that SDR5A1 gene expression in the follicular keratinocytes from the vertex area of the scalp seems not to be related to the differences in hair growth observed between normal men and women and hirsute patients. Further studies are needed to investigate the expression of the 5α-reductase genes in other scalp follicular compartments such as dermal papillae, and also in hair follicles from other body sites, in order to elucidate the mechanism of androgen action on the hair growth process and related diseases
Obtenção de folĂculos pilosos de pacientes hiperandrogĂȘnicas para avaliação da expressĂŁo gĂȘnica de receptores androgĂȘnicos e da 5alfa-redutase e 17HSD
09
ExpressĂŁo gĂȘnica das isoformas I e II da 5-alfa-redutase em folĂculos pilosos de escalpo de homens e mulheres normais e hiperandrogĂȘnicas
10
The 5 alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals
The aim of the present study was to determine the expression of the genes for type 1 (SDR5A1) and type 2 (SDR5A2) 5α-reductase isoenzymes in scalp hairs plucked from 33 hirsute patients (20 with polycystic ovary syndrome and 13 with idiopathic hirsutism) and compare it with that of 10 men and 15 normal women. SDR5A1 and SDR5A2 expression was estimated by RT-PCR using the gene of the ubiquitously expressed protein Ă2-microglobulin as an internal control. The results are expressed as arbitrary units in relation to Ă2- microglobulin absorbance (mean ± SEM). SDR5A2 expression was not detected in any hair samples analyzed in this study. No differences were found in SDR5A1 mRNA levels between men and normal women (0.78 ± 0.05 vs 0.74 ± 0.06, respectively). SDR5A1 gene expression in the cells of hair plucked from the scalp of normal women (0.85 ± 0.04) and of women with polycystic ovary syndrome (0.78 ± 0.05) and idiopathic hirsutism (0.80 ± 0.06) was also similar. These results indicate that SDR5A1 gene expression in the follicular keratinocytes from the vertex area of the scalp seems not to be related to the differences in hair growth observed between normal men and women and hirsute patients. Further studies are needed to investigate the expression of the 5α-reductase genes in other scalp follicular compartments such as dermal papillae, and also in hair follicles from other body sites, in order to elucidate the mechanism of androgen action on the hair growth process and related diseases
ExpressĂŁo gĂȘnica das isoformas I e II da 5-alfa-redutase em folĂculos pilosos de escalpo de homens e mulheres normais e hiperandrogĂȘnicas
10
Obtenção de folĂculos pilosos de pacientes hiperandrogĂȘnicas para avaliação da expressĂŁo gĂȘnica de receptores androgĂȘnicos e da 5alfa-redutase e 17HSD
09