PENGARUH EKSTRAK AQUEOUS KULIT DELIMA (Punica granatum) PERORAL TERHADAP MAKROFAG, FIBROBLAS DAN KOLAGEN PADA PENYEMBUHAN LUKA BAKAR TIKUS PUTIH

Background: Burn wound healing is a complex process, which involves the process of inflammation, oxidative stress, and infection. Therefore it is costly both to stabilize the general condition of the body, wound care itself, to prevent and to treat complications. Breakthrough is needed to solve this problem in an effective, safe, and affordable ways. One alternative of the breakthroughs is to use natural ingredients and Pomegranate is one of that ingredient which is widely studied today. Pomegranates have antioxidant effects, anti-inflammatory, anti-infective and anti-carcinogenic due to its content of polyphenols and derivatives, such ellagic acid, punicalagin, etc. Pomegranate polyphenol content higher than other fruits and it’s peel contain polyphenols pomegranate highest among other parts. It is known that the aqueous extract of pomegranate peel orally can stimulate healing of incision wound and pomegranate peel extract topically effective for burn wound healing. Research in the effect of oral pomegranate (Punica granatum) peel aqueous extract on burn wound healing of white rats have not been investigated. Objective: To proof the effect of oral pomegranate (Punica granatum) peel aqueous extract on macrophage, fibroblast, and collagen thickness burn wound healing of white rats. Methods: An experimental with post-test only control group. A total of 28 rats with deep burn wound, divided into four groups: two groups of rats which will be given pomegranate peel aqueous extract 50mg/kgbw/day orally examined at 3 (inflammatory phase) and 10 (proliferative phase) days, and two groups of rats which will not be given the aqueous extract of pomegranate peel oral, examined at 3 and 10 days too. Burn wound will undergo histologic examination with hematoxylin-eosin staining counted the number of macrophages, fibroblast density, and thickness of collagen. Data were analyzed with multivariate analysis of variance analysis (Manova). Results: The number of macrophage at 3 days was similar to the control, but at 10 days control increased significantly (P<0,05). The number of fibroblast was significantly higher at 3 days (P<0,005) but at 10 days was simmilar to the control. The collagen thickness at 3 days increased although not statistically significant to the control but at 10 days control decreased significantly (P<0,05). Conclussion : Oral pomegranate (Punica granatum) peel aqueous extract doesn’t decrease macrophage but inhibit its raising, increases fibroblast significantly and increases collagen thickness although not statistically significant

Pemberian Ekstrak Curcuma Longa L. Meningkatkan Proliferasi Sel MEF yang dilakukan Scratch Test

Abstract: Proliferation Enhancement of Curcuma Longa L. Extract Treatment in MEF Cell Cultres that Treated by Scratch Test. Wound prevalence is still high and becomes a problem of healing process. Improper wounds management can cause wound healing disturbance, so that the healing process will be longer. For this reason, alternative medicine is required to shorten healing process and reduce medical costs. This study aims to determine the effect of Curcuma longa L. extract on enhancement of mouse embryonic fibroblast cell proliferation carried out in vitro scratch test with the research method using a laboratory in vitro experimental design and 24 hour observation. Observations were carried out for 24 hours with variations in Curcuma longa L. extract concentration 2.5 ppm, 5 ppm, 10 ppm, 25 ppm and 50 ppm. The results showed that Curcuma longa L extract was able to accelerate the proliferation of mouse embryonic fbroblast cells which scratch test. After 24 hours, the highest gap closure were observed in mouse embryonic fbroblast cell culture using dose of 2.5 ppm of Curcuma longa L. extract administration. At a dose of ≥10 ppm it does not show proliferation in embryonic fbroblast mouse cells but causes toxicity to cells. Abstrak: Pemberian Ekstrak Curcuma Longa L. Meningkatkan Proliferasi Sel MEF yang dilakukan Scratch Test. Prevalensi luka yang tinggi masih menjadi permasalahan tersendiri dalam proses penyembuhan. Penanganan luka yang tidak tepat dapat menyebabkan gangguan penyembuhan sehingga proses penyembuhan menjadi panjang. Untuk itu perlu alternatif pengobatan yang dapat mempersingkat penyembuhan dengan biaya pengobatan yang lebih murah. Penetian ini bertujuan mengetahui pengaruh pemberian ekstrak Curcuma longa L. terhadap peningkatan proliferasi sel mouse embryonic fibroblast yang dilakukan scratch test secara in vitro dengan metode penelitian menggunakan desain eksperimen in vitro laboratoris dan dilakukan pengamatan selama 24 jam. Variasi konsentrasi ekstrak Curcuma longa L menggunakan dosis 2.5 ppm, 5 ppm, 10 ppm, 25 ppm dan 50 ppm. Hasil pengamatan menunjukkan bahwa ekstrak Curcuma longa L. mampu mempercepat proliferasi sel mouse embryonic fbroblast yang dilakukan scratch test dengan dosis optimum 2.5 ppm. Pada dosis ≥10 ppm tidak menunjukkan proliferasi pada sel mouse embryonic fbroblast tetapi menyebabkan toksisitas pada sel