6,118 research outputs found
Inducción rizogénica en estacas semileñosas de Berberidopsis corallina
- Latsague, M. ; Sáez, P.; Cifuentes, P.; Yáñez ,Y. Laboratorio de Ecofisiología Vegetal y Citogenética, Facultad de Recursos Naturales, Universidad Católica de Temuco, Chile.Berberidopsis corallina (michay rojo) es una especie endémica, catalogada como especie
“En Peligro de Extinción”. Su persistente disminución está asociada a tala y competencia
con Eucalyptus spp. y P. radiata. A raíz de continuas campañas realizadas por
organizaciones ambientales, se han enfocado esfuerzos en intentar conservar los bosques
nativos remanentes. Entre las herramientas de gestión a esta problemática se cuenta la
propagación vegetativa, como una forma de aumentar el numero de individuos y acortar el
período vegetativo, sin embargo, existe escasa información respecto a la propagación de
michay rojo, por lo que, el objetivo del estudio fue evaluar la respuesta al tratamiento de
enraizamiento de estacas semileñosas, como un aporte a la conservación de la especie. Las
estacas se colectaron en marzo del 2007 en el sector Villa las Araucarias, al Sur de la
Cordillera de Nahuelbuta, región de la Araucanía. Las estacas se trataron con Ácido Indol
Butírico (AIB) en distintas concentraciones (0, 500,1000 y 1500 ppm). Luego de cinco
meses en cama caliente, se obtuvo un 87% de enraizamiento total. La concentración 1000
ppm de AIB mostró los mejores resultados respecto al proceso de rizogénesis con un 90%
de enraizamiento, encontrando la mayor longitud de raíces en este mismo tratamiento con
un promedio de 13,64 cm. El mayor número de raíces se obtuvo en el tratamiento 1500
ppm de AIB con un valor promedio de 38,11 raíces por estaca. Se concluye que michay
rojo puede ser reproducido vegetativamente a través de la rizogénesis de estacas tratadas
con AIB
Rostral floor plate (flexural organ) secretes glycoproteins immunologically similar to subcommissural organ glycoproteins in dogfish (Scyliorhinus canicula) embryos
The subcommissural organ of vertebrates secretes glycoproteins into the cerebrospinal fluid of the third cerebral ventricle. This material polymerizes in Reissner's fiber. During ontogenetic development, besides the subcommissural organ, the ependyma lining the pontine flexure constitutes an additional Reissner's fiber-secreting gland named flexural organ. We have studied the secretion of the flexural organ and the subcommissural organ in dogfish (Scyliorhinus canicula) embryos using three different antisera and the lectins concanavalin A and wheat germ agglutinin. AFRU is an antiserum against the bovine Reissner's fiber; Ab-600 is an antiserum against 600 kDa dogfish subcommissural organ glycoproteins; and APSO is an antiserum against immunoaffinity purified bovine subcommissural organ secretory glycoproteins. These three antisera immunostained the flexural organ indicating that it contains epitopes similar to those present in bovine and dogfish subcommissural organ glycoproteins. It seems highly probable that the flexural or an and the subcommissural organ of dogfish embryos secrete similar compound(s). Other ependymal regions were also immunostained with Ab-600 and APSO antisera. Then, Reissner's fiber-like glycoproteins were transiently expressed by most embryonary ependymal cells. These glycoproteins might play a role in the development of the central nervous system of vertebrates. (C) 1997 Elsevier Science B.V
Cytomolecular identification of individual wheat-wheat chromosome arm associations in wheat-rye hybrids
Chromosome pairing in the meiotic metaphase I of wheatrye
hybrids has been characterized by sequential genomic
and fluorescent in situ hybridization allowing not only the
discrimination of wheat and rye chromosomes, but also the
identification of the individual wheat and rye chromosome
arms involved in the chromosome associations. The majority
of associations (93.8%) were observed between the wheat
chromosomes. The largest number of wheat-wheat chromosome
associations (53%) was detected between the A and D
genomes, while the frequency of B-D and A-B associations
was significantly lower (32 and 8%, respectively). Among the
A-D chromosome associations, pairing between the 3AL and
3DL arms was observed with the highest frequency, while
the most frequent of all the chromosome associations (0.113/
cell) was found to be the 3DS-3BS. Differences in the pairing
frequency of the individual chromosome arms of wheat-rye
hybrids have been discussed in relation to the homoeologous
relationships between the constituent genomes of
hexaploid wheat
Quantification of the secretory glycoproteins of the subcommissural organ by a sensitive sandwich ELISA with a polyclonal antibody and a set of monoclonal antibodies against the bovine Reissner's fiber
The subcommissural organ (SCO) is an ependymal brain gland that releases glycoproteins into the ventricular cerebrospinal fluid where they condense to form the Reissner's fiber (RF). We have developed a highly sensitive and specific two-antibody sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of the bovine SCO secretory material. The assay was based on the use of the IgG fraction of a polyclonal antiserum against the bovine RF as capture antibody and a pool of three peroxidase-labeled monoclonal antibodies that recognize non-overlapping epitopes of the RF glycoproteins as detection antibody. The detection limit was 1 ng/ml and the working range extended from 1 to 4000 ng/ml. The calibration curve, generated with RF glycoproteins, showed two linear segments: one of low sensitivity, ranging from 1 to 125 ng/ml, and the other of high sensitivity between 125 and 4000 ng/ml. This assay was highly reproducible (mean intra- and interassay coefficient of variation 2.2% and 5.3%, respectively) and its detectability and sensitivity were higher than those of ELISAs using exclusively either polyclonal or monoclonal antibodies against RF glycoproteins. The assay succeeded in detecting and measuring secretory material in crude extracts of bovine SCO, culture medium supernatant of SCO explants and incubation medium of bovine RF; however, soluble secretory material was not detected in bovine cerebrospinal fluid
Nonlinear absorption and nonlinear refraction: Maximizing the merit factors
Both nonlinear absorption and nonlinear refraction are effects that are potentially useful for a plethora of applications in photonics, nanophotonics and biophotonics. Despite substantial attention given to these phenomena by researchers studying the merits of disparate systems such as organic materials, hybrid materials, metal-containing molecules and nanostructures, it is virtually impossible to compare the results obtained on different materials when varying parameters of the light beams and different techniques are employed. We have attempted to address the problem by studying the properties of various systems in a systematic way, within a wide range of wavelengths, and including the regions of onephoton, two-photon and three-photon absorption. The objects of our studies have been typical nonlinear chromophores, such as π-conjugated molecules, oligomers and polymers, organometallics and coordination complexes containing transition metals, organometallic dendrimers, small metal-containing clusters, and nanoparticles of various kinds, including semiconductor quantum dots, plasmonic particles and rare-earth doped nanocrystals. We discuss herein procedures to quantify the nonlinear response of all of these systems, by defining and comparing the merit factors relevant for various applications
Searching for specific binding sites of the secretory glycoproteins of the subcommissural organ
The molecular organization of Reissner's fiber (RF), the structure of its proteins, and the permanent turnover of these proteins are all facts supporting the possibility that RF may perform multiple functions. There is evidence that CSF-soluble RF-glycoproteins may occur under physiological conditions. The present investigation was designed to investigate the probable existence within the CNS of specific binding sites for RF-glycoproteins. Three experimental protocols were used: (1) immunocytochemistry of the CNS of bovine fetuses using anti-idiotypic antibodies, raised against monoclonal antibodies developed against bovine RF-glycoproteins; (2) in vivo binding of the RF glycoproteins, perfusing into the rat CSF 125I-labeled RF-glycoproteins, or grafting SCO into a lateral ventricle of the rat; (3) in vitro binding of unlabeled RF-glycoproteins to rat and bovine choroid plexuses maintained in culture. One of the anti-idiotypic antibody generated by a Mab raised against RF-glycoproteins binds to choroidal cells. Furthermore, binding of RF-glycoproteins to the rat choroid plexus was obtained when: (1) the choroid plexus was cultured in the presence of unlabeled RF-glycoproteins; (2) the concentration of soluble RF-glycoproteins in the CSF was increased by isografting SCOs into a lateral ventricle; (3) radiolabeled glycoproteins were perfused into the ventricular CSF. This evidence suggests that the apical plasma membrane of the ependymal cells of the choroid plexus has specific binding sites for RF-glycoproteins, of unknown functional significance. The radiolabeled RF-glycoproteins perfused into the rat CSF also bound to the paraventricular thalamic nucleus, the floor of the Sylvian aqueduct and of the rostral half of the fourth ventricle, and title meninges of the brain and spinal cord. The labeling of the paraventricular thalamic nucleus points to a functional relationship between this nucleus and the SCO. The possibility that; the SCO may be a component of the circadian timing system is discussed. Microsc. Res. Tech. 52:541-551, 2001. (C) 2001 Wiley-Liss, Inc
Exceptionally large two- and three-photon absorption cross-sections by OPV organometalation
Oligo(p-phenylenevinylene)s (OPVs) containing up to 8 PV units and end-functionalized by ruthenium alkynyl groups have been prepared and their nonlinear absorption properties assessed using the Z-scan technique and employing low repetition rate femtosecond pulses. Exceptionally large two-photon absorption (ca. 12 500 GM at 725 nm) and three-photon absorption cross sections (ca. 1.6 × 10⁻⁷⁶ cm⁶s² at 1100 nm) are found for the 8PV-containing example, highlighting the potential of an “organometalation” approach to NLO-efficient organic materialsWe thank the Australian Research Council (ARC), the National
Natural Science Foundation of China (51432006), the Chinese
Government Ministry of Education, the Chinese Government State
Administration of Foreign Experts Affairs (111 Project: B13025), and
the National Science Centre of Poland (grant 2013/10/A/ST4/00114)
for financial support. M. P. C. thanks the ARC for an Australian
Research Fellowship and C. Q. thanks CONICYT (Chile) for a Chile
PhD Scholarship Abroad
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