Effects of Dietary Supplementation of Some Antioxidants on Liver Antioxidant Status and Plasma Biochemistry Parameters of Heat-Stressed Quail

This study aimed to compare the dietary supplementation of oleuropein (O) and α-tocopherol acetate (TA) alone or with organic selenium (Se) on liver antioxidant status and some plasma biochemistry parameters in Japanese quails reared under heat stress (HS). A total of 800, two-weeks old quails were kept in wire cages in the temperature-controlled rooms at either 22°C or 34°C for 8 h/d and fed on a basal diet (NC) or the diets supplemented with TA (TA200) or O (O200) at 200 mg/kg alone or with OSe (TA200+OSe and O200+OSe) to the NC diet. HS decreased the total antioxidant status (TAS) and increased the total oxidative stress (TOS) and oxidative stress index (OSI) of liver compared to thermoneutral temperature (TN). The TA200, O200, TA200+OSe and O200+OSe diets increased TAS and decreased TOS of liver compared to those of quails fed NC. OSI was decreased by the TA200, O200 and TA200+OSe diets compared to NC and O200+OSe diets. HS reduced plasma albumin (A) and total protein (TP) concentrations, on the other hand, increased plasma glucose (G), total cholesterol (CHO) and triglyceride (TG) levels compared to TN. The TA200, O200, TA200+OSe and O200+OSe diets reduced plasma total CHO and TG levels and increased plasma A level. The TA200 and TA200+OSe diets reduced plasma G level and increased plasma TP levels compared to those of quails fed the other diets. In conclusion, dietary supplementation of vitamin E and oleuropein alone or with organic selenium is necessary to remove the negative effects of heat stress on liver antioxidant status and some plasma parameters of quails

Myelin basic protein profile of central nervous system in experimentally induced demyelination and remyelination

Objective: The aim of the present study was to assess myelin basic protein (MBP) profiles of central nervous system in experimentally induced demyelination and remyelination

Effect of lairage time after short distance transport on some biochemical stress parameters and meat quality of Karayaka lambs

Sheep are transported for various causes such as sale, breeding and slaughter. Several potential stressors which impair the meat quality affect sheep during the transport. Lairage is a common commercial practise to get rid of transportation stress before slaughter

Effects of Stocking Density of Lambs on Biochemical Stress Parameters and Meat Quality Related to Commercial Transportation

The aim of this study was to evaluate the effects of stocking density (0.20 and 0.27 m(2)/lamb) during transportation on certain biochemical stress parameters and meat quality characteristics of fifty-five Karayaka lambs divided into two groups. The front compartment of the trailer was loaded at high stocking density (HD: 0.20 m(2)/lamb; n=33) and the remainder was loaded at low stocking density (LD: 0.27 m(2)/lamb; n=22). The distance was approximately 130 km and duration was 2 h 15 min. Blood samples were taken just before and just after transportation. Glucose (P0.05). Transportation with a stocking density of 0.20 m(2)/lamb resulted in higher stress responses in lambs compared with a stocking density of 0.27 m(2)/lamb. However, increased stress in the HD group did not lead to any adverse effects on meat quality characteristics. In the determination of stocking density, a compromise that gives animal welfare and commercial objectives equal status would improve the current situation

EFFECTS OF STOCKING DENSITY OF LAMBS ON BIOCHEMICAL STRESS PARAMETERS AND MEAT QUALITY RELATED TO COMMERCIAL TRANSPORTATION

The aim of this study was to evaluate the effects of stocking density (0.20 and 0.27 m(2)/lamb) during transportation on certain biochemical stress parameters and meat quality characteristics of fifty-five Karayaka lambs divided into two groups. The front compartment of the trailer was loaded at high stocking density (HD: 0.20 m(2)/lamb; n=33) and the remainder was loaded at low stocking density (LD: 0.27 m(2)/lamb; n=22). The distance was approximately 130 km and duration was 2 h 15 min. Blood samples were taken just before and just after transportation. Glucose (P0.05). Transportation with a stocking density of 0.20 m(2)/lamb resulted in higher stress responses in lambs compared with a stocking density of 0.27 m(2)/lamb. However, increased stress in the HD group did not lead to any adverse effects on meat quality characteristics. In the determination of stocking density, a compromise that gives animal welfare and commercial objectives equal status would improve the current situation

Wpływ sedacji w trakcie echokardiografii przezprzełykowej na zmienność rytmu serca: porównanie sedacji za pomocą hipnozy z sedacją farmakologiczną

Background: There is no ideal sedation technique that can be used during transoesophageal echocardiography (TEE), and the data concerning the effects of available sedation techniques on heart rate variability (HRV) are limited. Aim: To compare the effects of sedation through hypnotherapy with medical sedation achieved by midazolam on HRV. Methods: We recruited 76 patients with an indication of TEE; the age range was 18–83 years. In Group T there were 26 patients who had the procedure under topical pharyngeal anaesthesia, in Group D there were 23 patients who received midazolam, and in Group H there were 27 patients receiving hypnosis. All patients had an IV access; throughout the procedure heart rate, rhythm electrocardiography, and peripheric O2 saturation were monitored with a non-invasive monitor, and blood pressure measurements were taken every 3 min. Rhythm Holter recordings were obtained from all patients and TEE was performed. Results: When time domain parameters for HRV were compared in all three groups, the hypnosis group had significant increases in pNN50 and RMSSD compared to Groups D and T (p < 0.05). As concerns frequency domain parameters, there were no significant differences between groups where low frequency (LF) was decreased in hypnosis group and high frequency (HF) was increased (p > 0.05). However, LF/HF was decreased statistically significantly (p < 0.05) when compared with the midazolam group. Conclusions: Contrary to standard sedation in TEE patients, when hypnosis is used autonomic cardiac tone is modified to a significant extent. Hypnotic sedation achieves this by increasing the parasympathetic activity, decreasing the sympathetic activity, and changing the sympathovagal interaction balance.  Wstęp: Nie ma idealnej metody sedacji, która mogłaby być stosowana w trakcie echokardiografii przezprzełykowej (TEE), a dane dotyczące wpływu dostępnych metod sedacji na zmienność rytmu serca (HRV) są ograniczone. Cel: Badanie przeprowadzono w celu porównania wpływu na HRV sedacji uzyskanej metodą hipnoterapii i sedacji farmakologicznej z zastosowaniem midazolamu. Metody: Do badania włączono 76 chorych ze wskazaniami do wykonania TEE, w wieku 18–83 lat. Grupa T obejmowała 26 chorych, u których badanie wykonano po zastosowaniu miejscowego znieczulenia gardła, grupa D — 23 chorych, którym podano midazolam, a grupa H — 27 chorych, których poddano hipnozie. U wszystkich pacjentów zapewniono dostęp dożylny. Przez cały czas trwania badania monitorowano częstość rytmu serca, zapis elektrokardiograficzny i saturację tlenem krwi obwodowej metodą nieinwazyjną; pomiary ciśnienia tętniczego wykonywano co 3 minuty. U wszystkich pacjentów uzyskano zapis rytmu serca metodą Holtera i przeprowadzono TEE. Wyniki: Porównując parametry HRV uzyskane w trzech grupach, stwierdzono, że w grupie poddanej hipnozie wartości pNN50 i RMSSD były istotnie wyższe niż w grupach D i T (p < 0,05). Na podstawie oceny parametrów analizy częstotliwościowej nie wykazano istotnych różnic między osobami poddanymi hipnozie, u których stwierdzono zmniejszenie mocy widma w zakresie niskich częstotliwości (LF) i zwiększenie w zakresie wysokich częstotliwości (HF) (p > 0,05). Jednak zaobserwowano statystycznie istotną (p < 0,05) redukcję współczynnika LF/HF w porównaniu z grupą, której podano midazolam. Wnioski: W przeciwieństwie do standardowej metody sedacji u chorych poddanych TEE stosowanie hipnozy wiązało się z istotną zmianą napięcia autonomicznego układu nerwowego serca. Sedacja za pomocą hipnozy powodowała zwiększenie aktywności przywspółczulnej, zmniejszenie aktywności współczulnej i zmianę balansu interakcji między układem współczulnym a przywspółczulnym (nerw błędny)

Evaluation of serum cystatinC concentrations in dogs infected with Dirofilaria immitis

To assess glomerular filtration rate (GFR) in dogs naturally infected by D. immitis new renal marker serum cystatinC (sCysC) was measured with canine CysC ELISA kit. Twenty infected microfilaremic-seropositive and 20 amicrofilaremic and seronegative dogs evaluated in the study. Serum urea and creatinine (sCre) concentrations were measured in both groups. Mean sCysC concentrations in infected dogs were detected as 2.40 mg/L and 2.30 mg/L in controls. Mean serum urea 9.97 mmol/L and sCre 116.24 mu mol/L concentrations in infected and in the uninfected group detected as 11.92 mmol/L and 128.62 mu mol/L, respectively. Including mean sCysC and sCre concentrations with mean serum Urea:Cre ratio were not statistically differed between the groups. On the other hand, serum urea concentrations were statistically differed between groups. Based on the results of the present study, it is concluded that, sCysC concentrations seemed to be not altered in dogs with D. immitis infection

The effects of aging on the central nervous system steroid profiles and myelin basic protein in rats

The aim of this study was to investigate the effects of aging on the central nervous system steroid and myelin basic protein (MBP) profiles. Forty-seven male Sprague-Dawley rats (newborn, 1, 6, 12 and 24-months-old) were studied. Tissues were obtained from the cerebellum and parietal, frontal, temporal cortex of the central nervous system of the rats for steroid extraction. The estradiol, progesteron, testosterone and dehydroepiandrosterone (DHEA) levels were measured by Enzyme Linked Immunosorbent Assay (ELISA). The average levels of estradiol (pg/g), progesteron (ng/g), DHEA (ng/g) and testosterone (ng/g) in the brain tissues were respectively 24.29, 4.59, 0.27, 0.92 in the newborn-rats; 4.18 +/- 1.10, 1.54 +/- 0.30, 0.28 +/- 0.01, 0.57 +/- 0.10 in the 1 month-old-rats; 11.02 +/- 1.10, 2.96 +/- 0.30, 0.27 +/- 0.01, 0.61 +/- 0.10 in the 6 month-old-rats; 15.80 +/- 1.10, 4.80 +/- 0.30, 0.28 +/- 0.10, 0.67 +/- 0.10 in the 12 month-old-rats; 20.07 +/- 1.10, 4.12 +/- 0.30, 0.28 +/- 0.01, 0.55 +/- 0.10 in the 24 month-old-rats. The myelin basic protein levels were determined by immunohistochemical staining and an elevation was observed in conjunction with the aging process. The results of the study indicate that the alterations in MBP, DHEA, progesterone, testosterone and estrodiol concentrations in the central nervous system of the rats during aging can be considered fundamental for future animal and human studies. (C) 2012, Editrice Kurti