AMASS

AMASS documents and augments 'Amass Radiant Heretics', a 17-hour live internet stream that took place on midwinters night 2020. The artist's book is a collision of the contemporary experience of digital togetherness with residual ideas of ritual and event. AMASS, is fire and light, death and rebirth, a celebration, a gathering, a reading, a performance, and a happening; an evocation of the new underworld. The initial all-night online event included original practice research by Caleb Madden within a digital curation by The Antivoid Alliance (Jim Smithyes, Caleb Madden and Grant Cieciura) incorporating the work of 30+ other artists

Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor

The THO complex is a key factor in co-transcriptional formation of export-competent messenger ribonucleoprotein particles, yet its structure and mechanism of chromatin recruitment remain unknown. In yeast, this complex has been described as a heterotetramer (Tho2, Hpr1, Mft1, and Thp2) that interacts with Tex1 and mRNA export factors Sub2 and Yra1 to form the TRanscription EXport (TREX) complex. In this study, we purified yeast THO and found Tex1 to be part of its core. We determined the three-dimensional structures of five-subunit THO complex by electron microscopy and located the positions of Tex1, Hpr1, and Tho2 C-terminus using various labelling techniques. In the case of Tex1, a β-propeller protein, we have generated an atomic model which docks into the corresponding part of the THO complex envelope. Furthermore, we show that THO directly interacts with nucleic acids through the unfolded C-terminal region of Tho2, whose removal reduces THO recruitment to active chromatin leading to mRNA biogenesis defects. In summary, this study describes the THO architecture, the structural basis for its chromatin targeting, and highlights the importance of unfolded regions of eukaryotic proteins.Ministerio de Ciencia e Innovación BFU2010- 15703/BMC, BFU2006-0526

Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor.

The THO complex is a key factor in co-transcriptional formation of export-competent messenger ribonucleoprotein particles, yet its structure and mechanism of chromatin recruitment remain unknown. In yeast, this complex has been described as a heterotetramer (Tho2, Hpr1, Mft1, and Thp2) that interacts with Tex1 and mRNA export factors Sub2 and Yra1 to form the TRanscription EXport (TREX) complex. In this study, we purified yeast THO and found Tex1 to be part of its core. We determined the three-dimensional structures of five-subunit THO complex by electron microscopy and located the positions of Tex1, Hpr1, and Tho2 C-terminus using various labelling techniques. In the case of Tex1, a β-propeller protein, we have generated an atomic model which docks into the corresponding part of the THO complex envelope. Furthermore, we show that THO directly interacts with nucleic acids through the unfolded C-terminal region of Tho2, whose removal reduces THO recruitment to active chromatin leading to mRNA biogenesis defects. In summary, this study describes the THO architecture, the structural basis for its chromatin targeting, and highlights the importance of unfolded regions of eukaryotic proteins

Antivoid Alliance - SPAMJAM:Social Parrhesia As Musical JAM- Radio/online broadcast, Radiophrenia festival 2023

If parrhesia once described the bravery of a solo voice against a single force. Then a multitudinous oppression should be met with a chorus of dissent. A collective jamming of the signal; a co-produced glitch, a dissonant polyrhythm set against a discordant song. In defiance of the individualisation of our call the response to repression should be played amongst allies. A rehearsal of a people yet to come in a place still being built, an unspokenword night of joyous resistance, of reverberation and resonance, of Social Parrhesia As Musical JAM.Recorded live at Brighton Electric, Sunday 20 June 2021, Brighton. Accepted and broadcast as part of Radiophrenia festival 202

Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations.

For high-throughput structural studies of protein complexes of composition inferred from proteomics data, it is crucial that candidate complexes are selected accurately. Herein, we exemplify a procedure that combines a bioinformatics tool for complex selection with in vivo validation, to deliver structural results in a medium-throughout manner. We have selected a set of 20 yeast complexes, which were predicted to be feasible by either an automated bioinformatics algorithm, by manual inspection of primary data, or by literature searches. These complexes were validated with two straightforward and efficient biochemical assays, and heterologous expression technologies of complex components were then used to produce the complexes to assess their feasibility experimentally. Approximately one-half of the selected complexes were useful for structural studies, and we detail one particular success story. Our results underscore the importance of accurate target selection and validation in avoiding transient, unstable, or simply nonexistent complexes from the outset

Vascular plants of lempis nature reserve in the augustow forest (ne poland)

During the survey of Łempis Nature Reserve (Augustów Forest, NE Poland) 353 species of vascular plants were recorded, including 32 considered threatened with extinction in Poland (7 species from the Polish “red data book” and 32 species from the Polish “red list”). Among them there were 41 species protected under the Polish law, including 19 strictly protected species and four species listed in the Annex II of the EU Habitats Directive. Ten orchid species were recorded, including the most valuable plants of the nature reserve – Cypripedium calceolus, Hammarbya paludosa, Liparis loeselii and Listera cordata. Among 32 noted Cyperaceae species (including 26 members of the Carex genus), there was a number of threatened plants, e.g. Baeothryon alpinum, Carex chordorrhiza, C. loliacea and Eriophorum gracile. Other rare species included Agrimonia pilosa, Cladium mariscus, Laserpitium latifolium, Linnaea borealis, Potamogeton gramineus and Pulsatilla patens. The presence of anthropophytes was significant (4.5% of the flora), probably due to the past forest management practices as well as proximity of settlements. The extremely rich flora, along with pristine wetland ecosystems (mesotrophic lakes, various mire forests and fens), places the Łempis Nature Reserve among the most valuable nature reserves in Poland