Identification, cloning, expression analysis and functional characterization of genes expressed early in Loblolly pine embryogenesis

Ph.D

Isolation of 'full-length' cDNA clone using SMART(TM) cDNA and a biotin-streptavidin bead system

"November 1999.""Submitted to BioTechniques.

An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.)

"September 2001."Submitted to Plant Physiolog

Differential display: a tool to follow natural and somatic embryo development in loblolly pine

"July 1997.""Submitted to Tappi R and D Division Biological Sciences Symposium, San Francisco, California, October 20-23, 1997.

Sleep/Wake Behavior and EEG Signatures of the TgF344-AD Rat Model at the Prodromal Stage

Transgenic modification of the two most common genes (APPsw, PS1ΔE9) related to familial Alzheimer’s disease (AD) in rats has produced a rodent model that develops pathognomonic signs of AD without genetic tau-protein modification. We used 17-month-old AD rats (n = 8) and age-matched controls (AC, n = 7) to evaluate differences in sleep behavior and EEG features during wakefulness (WAKE), non-rapid eye movement sleep (NREM), and rapid eye movement sleep (REM) over 24-h EEG recording (12:12h dark–light cycle). We discovered that AD rats had more sleep–wake transitions and an increased probability of shorter REM and NREM bouts. AD rats also expressed a more uniform distribution of the relative spectral power. Through analysis of information content in the EEG using entropy of difference, AD animals demonstrated less EEG information during WAKE, but more information during NREM. This seems to indicate a limited range of changes in EEG activity that could be caused by an AD-induced change in inhibitory network function as reflected by increased GABAAR-β2 expression but no increase in GAD-67 in AD animals. In conclusion, this transgenic rat model of Alzheimer’s disease demonstrates less obvious EEG features of WAKE during wakefulness and less canonical features of sleep during sleep

Annual Program Review Forest Biology

"March 21-22, 1995."Mass clonal propagation of improved conifers: project F010 / Gerald Pullman, John Cairney, Shujun Chang ; Mass clonal propagation of improved conifers - initiation / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation: March 1995 Update / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: growth kinetics of loblolly pine early-stage embryo suspensions / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: germination of somatic embryos / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: conversion of loblolly pine somatic embryo germinants to established seedlings / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: osmotic measurements during zygotic embryogenesis in loblolly pine / Gerald Pullman, Greg Eley ; Development of a direct ELISA for indole-3-acetic acide / Vincent Ciavatta, Ron Dinus, Jeff Dean ; Mass clonal propagation of genetically improved and engineered hardwoods: project F011 / Ronald J. Dinus, Camille J. Stephens, Shujun Chang -- Slide Material

Annual program review, Forest Biology, March 25-26, 1997

"March 25-26, 1997."Mass clonal propagation of improved conifers: project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation and culture survival / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: long-term storage of loblolly pine somatic embryo cultures in liquid nitrogen / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman, Paul Montello, Mike Cunningham ; Mass clonal propagation of improved conifers: progress in protocol development and targets / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - elemental analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman, Mike Buchanan, Yolanda Powell ; Mass clonal propagation of improved conifers: antibody markets, straining procedures and image analysis for characterizing pinus taeda embryogenesis / Gary Peter, Levi Barclay ; Mass clonal propagation of improved conifers: construction of cDNA libraries from zygotic embryos of pinus taeda / Gary Peter / Gerald Pullman ; Differential display as a tool to understand and improve somatic embryogenesis / John Cairney ; Monitoring gene expression in somatic embryos of an elite genotype of loblolly pine / John Cairney and Barbara Johns ; Differential display to compare gene expression in loblolly pine zygotic and somatic embryos / Vincent Ciavatta, Jerry Pullman and John Cairney ; Analysis of somatic embryo development using differential display: rapid identification, cloning and sequencing of marker bands by an improved method / Nanfei Xu, Jerry Pullman, and John Cairney ; Preliminary comparison of zygotic and somatic embryo development using differential display / Nanfei Xu, Jerry Pullman and John Cairney ; Gene expression during loblolly pine embryogenesis: drought-inducible genes also expressed in pine cell suspension culture / Ranjan Perera and John Cairney ; Mass clonal propagation of genetically improved and engineered hardwoods: project F011 / Gerald Pullman ... [et al.] ; Agrobacterium tumefaciens-mediated transformation of populus deltoides leaf sections / Ronald J. Dinus ... [et al.] ; Regulation of expression of a stress-related gene: promoter and 3' UTR analysis / Luis Destefano and John Cairney ; Identification of a plant enzyme which activates a regulatory peptide / John Cairney ... [et al.] ; Three year research plan for mass clonal propagation of genetically improved and engineered hardwoods 1997-2000 / Gary Peter, John Cairney, Gerald Pullman -- Slide Material

Annual program review, forest biology, March 26-27, 1998

"March 26-27, 1998."Mass clonal propagation of improved conifers: project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation and culture survival ... Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers-embryo development improvements due to modification of plate size and wrapping materials ... Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers: the effects of increasing ABA concentration and extending culture time on quality and quantity of cotyledonary somatic embryos of loblolly pine / Gerald Pullman and Xiaorong Feng ; Mass clonal propagation of improved conifers: optimizing plating methods for somatic embryo yields and automated embryo counting / Gary Peter, Teresa Vales ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - elemental analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of softwoods: functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Mass clonal propagation of softwoods: update - functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Mass clonal propagation of improved conifers: the role of the suspensor in early embryo development. I. Differential gene expression in the suspensor of zygotic embryos / John Mackay, Heidi Schindler, Jerry Pullman ; Externally funded research in 1997-1998 supporting F010 / John Cairney ... [et al.] ; Gene expression in loblolly pine embryos: cDNA cloning, high density array and gene expression detection / Nanfei Xu ... [et al.] ; Isolation and characterization of "pinine", a vicilline life seed storage protein from loblolly pine (pinus taeda) / Ranjan Perera ... [et al.] ; Culture cycling and tissue culture decline: pH cycling in loblolly pine suspension cultures and associated gene expression / Michael Sullivan ... [et al.] ; cDNA cloning of mRNAs present early in zygotic embryo development / Vincent Ciavatta, Gerald Pullman and John Cairney ; Combined effects of activated carbon and pH on ionic composition and 2,4-D availability in a tissue culture medium / S.C. Van Winkle, G. S. Pullman ; Mass clonal propagation of genetically improved hardwoods: project F011 / Gary Peter ... [et al.] ; F011 mass clonal propagation of genetically improved hardwoods / Gary Peter ... [et al.] ; F011: mass clonal propagation of genetically improved hardwoods: establishing and improving the zinnia elegans tracheary element differentiation system at IPST / Gary Peter, Karen Floyd, Chris Ricker ; Externally funded research in 1997-1998 supporting F011 / John Cairney ... [ et al.] ; Gene expression in trees: sequences affecting stability/translatability of mRNA / Luis Destefano-Beltran and John Cairney ; Gene regulation in woody plants: identification of a plant enzyme which activates a regulatory peptide / Luis Destefano ... [et al.]

Annual program review, forest biology

"March 25-26, 1999."Mass clonal propagation of improved conifers: status report for project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation / Gerald Pullman ... [et al.] ; Variation in initiation and the number of zygotic embryos in the seed / John MacKay ... [et al.] ; Survival of initiated cultures after direct transfer into liquid media: the effect of maltose, auxin type, and genotype / Gary Peter , Teresa Vales ; Embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman , Paul Montello , Xiaorong Feng ; Optimizing the percent of embryos that develop into cotyledonary embryos: the role of plating density and ABA concentration / Gary Peter , Teresa Vales ; Field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman , Paul Montello , Mike Cunningham ; Analysis of free amino acids, nitrate, and ammonium in zygotic female gametophyte and embryo tissues / Gerald Pullman ... [et al.] ; Functional analysis of pinus taeda zygotic embryo germination: the effect of partial drying and the acquisition of desiccation tolerance / Gary Peter ... [et al.] ; Gene expression in pine embryos; developing tools / John Cairney , Gerald Pullman , John MacKay ; Differential gene expression in the suspensor of zygotic embryos / John MacKay ... [et al.] ; Analysis of a late stage-specific clone of loblolly pine by a non-radioactive method of cDNA labeling and detection / Barbara Johns and John Cairney ; Externally funded research in 1998-1999 supporting F010 / John Cairney ... [et al.] ; Developing reliable systems for evaluating gene expression using DNA arrays / Lin Ge , Jerry Pullman and John Cairney ; Early-specific gene expression in loblolly pine zygotic and somatic embryos: cDNS cloning, sequencing and expression analysis / Vincent Ciavatta , Gerald Pullman , John Cairney ; Fundamental biological mechanisms: improved stem growth rates and fiber properties: status reports for project F011 / Gary Peter ... [et al.] ; Externally funded research in 1998-1999 supporting F011 / Gary Peter ... [et al.] ; Transformation methods for southern pine: building an efficient transformation method for loblolly pine / Gary Peter ... [et al.] ; Environmental and genetic regulation of microfibril angle in southern pine: determination of the microfibril angle in the s2 layer of pinus taeda using differential interference contrast microscopy: validation of the method / Douglas Benton , Keith Bennett , Gary Peter ; Trees containing built-in pulping catalysts / Jerry Pullman ... [et al.] ; Lignin reactivity during pulping and bleaching of CAD-deficient pine / John MacKay ... [et al.] ; IPST forest biology research proposals ; Student research -- Slide Material