16 research outputs found
Green Alga Ulva spp. Hydrolysates and Their Peptide Fractions Regulate Cytokine Production in Splenic Macrophages and Lymphocytes Involving the TLR4-NFkB/MAPK Pathways
Hydrolysates of food protein sources have immunomodulatory effects, which are of interest
for use as functional foods. In this study, we have characterized the immune regulatory effect
on rat splenocytes, macrophages and T lymphocytes of Ulva spp. hydrolysates and their peptide
fractions with or without in vitro gastrointestinal digestion and/or ultrafiltration. IL-10 was induced
in almost all conditions and cell types obtained from wild type animals. The induction was in
general increased by ultrafiltration and in vitro gastrointestinal digestion. TNF was also induced in
basal conditions. In turn, TNF and IFN-
production was attenuated by the hydrolysate products
in lipopolysaccharide or concanavalin A immune stimulated cells. Inhibitors for the activation
of NF B, MAPK p38 and JNK inhibited IL-10 induction in rat splenocytes. The response was
dramatically attenuated in TLR4-/- cells, and only modestly in TLR2-/- cells. Food peptides
from Ulva spp. genus exert anti-inflammatory effects in immune cells mediated by TLR4 and NF B.
Similarity with the immunomodulatory profile of protein hydrolysates from other sources suggests a
common mechanism.This work was supported by funds from the Ministry of Economy and Competitivity, partly with
Fondo Europeo de Desarrollo Regional FEDER funds [SAF2017-88457-R, AGL2017-85270-R, BFU2014-57736-P,
AGL2014-58883-R] and by Junta de AndalucĂa [CTS235, CTS164]. C.H.-C. and R.G.-B. were supported by
the University of Granada (Contrato Puente ProgramâPlan Propio) and the Ministry of Education [Spain],
respectively. CIBERehd is funded by Instituto de Salud Carlos III
Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota
Based on their composition, marine algae, and namely red seaweeds, are good potential functional foods. Intestinal mucosal barrier function refers to the capacity of the intestine to provide adequate containment of luminal microorganisms and molecules. Here, we will first outline the component of seaweeds and will summarize the effects of these on the regulation of mucosal barrier function. Special attention will be paid to unique components of red seaweeds: proteins and derived peptides (e.g., phycobiliproteins, glycoproteins that contain âcellulose binding domainsâ, phycolectins and the related mycosporine-like amino acids) together with polysaccharides (e.g., floridean starch and sulfated galactans, such as carrageenans, agarans and âdl-hybridâ) and minerals. These compounds have been shown to exert prebiotic effects, to regulate intestinal epithelial cell, macrophage and lymphocyte proliferation and differentiation and to modulate the immune response. Molecular mechanisms of action of peptides and polysaccharides are starting to be elucidated, and evidence indicating the involvement of epidermal growth factor receptor (EGFR), insulin-like growth factor receptor (IGFR), Toll-like receptors (TLR) and signal transduction pathways mediated by protein kinase B (PKB or AKT), nuclear factor-ÎșB (NF-ÎșB) and mitogen activated protein kinases (MAPK) will also be summarized. The need for further research is clear, but in vivo experiments point to an overall antiinflammatory effect of these algae, indicating that they can reinforce membrane barrier function
Anti-diabetic properties of brewerâs spent yeast peptides. In vitro, in silico and ex vivo study after simulated gastrointestinal digestion
Brewerâs spent yeast (BSY) hydrolysates are a source of antidiabetic peptides. Nevertheless, the impact of
in vitro gastrointestinal digestion of BSY derived peptides on diabetes has not been assessed. In this study,
two BSY hydrolysates were obtained (H1 and H2) using ÎČ-glucanase and alkaline protease, with either 1 h
or 2 h hydrolysis time for H1 and H2, respectively. These hydrolysates were then subjected to simulated
gastrointestinal digestion (SGID), obtaining dialysates D1 and D2, respectively. BSY hydrolysates inhibited
the activity of α-glucosidase and dipeptidyl peptidase IV (DPP-IV) enzymes. Moreover, although D2 was
inactive against these enzymes, D1 IC50 value was lower than those found for the hydrolysates.
Interestingly, after electrophoretic separation, D1 mannose-linked peptides showed the highest
α-glucosidase inhibitory activity, while non-glycosylated peptides had the highest DPP-IV inhibitory
activity. Kinetic analyses showed a non-competitive mechanism in both cases. After peptide identification,
GILFVGSGVSGGEEGAR and IINEPTAAAIAYGLDK showed the highest in silico anti-diabetic activities
among mannose-linked and non-glycosylated peptides, respectively (AntiDMPpred score: 0.70 and 0.77).
Molecular docking also indicated that these peptides act as non-competitive inhibitors. Finally, an ex vivo
model of mouse jejunum organoids was used to study the effect of D1 on the expression of intestinal epithelial
genes related to diabetes. The reduction of the expression of genes that codify lactase, sucraseisomaltase
and glucose transporter 2 was observed, as well as an increase in the expression of Gip
(glucose-dependent insulinotropic peptide) and Glp1 (glucagon-like peptide 1). This is the first report to
evaluate the anti-diabetic effect of BSY peptides in mouse jejunum organoidsAgencia Nacional de PromociĂłn CientĂfica y TecnolĂłgica (ANPCyT) - Argentina (Project PICT-2020-Serie A-1985)Agencia Santafesina de Ciencia TecnologĂa e InnovaciĂłn â Argentina (Res. 132/23-Project PEICID-2022-133)Ministerio de Ciencia e InnovaciĂłn MICINN grant no. PID2020-112768RB-I00Instituto de Salud Carlos III, grant no. PI21/00952Junta de AndalucĂa and FEDER Grants A-AGR-468-UGR20 and P20-0069
Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota
Based on their composition, marine algae, and namely red seaweeds, are good potential functional foods. Intestinal mucosal barrier function refers to the capacity of the intestine to provide adequate containment of luminal microorganisms and molecules. Here, we will first outline the component of seaweeds and will summarize the effects of these on the regulation of mucosal barrier function. Special attention will be paid to unique components of red seaweeds: proteins and derived peptides (e.g., phycobiliproteins, glycoproteins that contain âcellulose binding domainsâ, phycolectins and the related mycosporine-like amino acids) together with polysaccharides (e.g., floridean starch and sulfated galactans, such as carrageenans, agarans and âdl-hybridâ) and minerals. These compounds have been shown to exert prebiotic effects, to regulate intestinal epithelial cell, macrophage and lymphocyte proliferation and differentiation and to modulate the immune response. Molecular mechanisms of action of peptides and polysaccharides are starting to be elucidated, and evidence indicating the involvement of epidermal growth factor receptor (EGFR), insulin-like growth factor receptor (IGFR), Toll-like receptors (TLR) and signal transduction pathways mediated by protein kinase B (PKB or AKT), nuclear factor-ÎșB (NF-ÎșB) and mitogen activated protein kinases (MAPK) will also be summarized. The need for further research is clear, but in vivo experiments point to an overall antiinflammatory effect of these algae, indicating that they can reinforce membrane barrier function
Chemical composition, nutritional and antioxidant properties of the red edible seaweed Porphyra columbina
Proximate composition, fatty acids and amino acid profiles and nutritional (chemical score, protein digestibility, PDCAAS and mineral dialyzability) and antioxidant properties (TEAC, DPPH and power reduction) from Porphyra columbina were evaluated. Total dietary fiber (48.02±1.13g/100g dry weight) and protein (24.61±0.21g/100g dry weight) were the two most abundant components in this seaweed. The main saturated and unsaturated fatty acids were C16:0 and C20:5 (n-3), respectively. The limiting amino acid was tryptophan with a chemical score of 57%. Protein digestibility was 74.33±3.0%. Porphyra columbina has high mineral content with good Na/K relationship and medium value of potential mineral accessibility (P, Ca and Zn dializability: 18.75±0.01, 17.62±0.16 and 16.70±0.44, respectively). The highest antioxidant properties were obtained with an acetone/water extraction system. This work provides important information about chemical composition and nutraceutical new properties of P. columbina. © 2014 Informa UK Ltd. All rights reserved.Peer Reviewe
Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota
Based on their composition, marine algae, and namely red seaweeds, are good potential functional foods. Intestinal mucosal barrier function refers to the capacity of the intestine to provide adequate containment of luminal microorganisms and molecules. Here, we will first outline the component of seaweeds and will summarize the effects of these on the regulation of mucosal barrier function. Special attention will be paid to unique components of red seaweeds: proteins and derived peptides (e.g., phycobiliproteins, glycoproteins that contain âcellulose binding domainsâ, phycolectins and the related mycosporine-like amino acids) together with polysaccharides (e.g., floridean starch and sulfated galactans, such as carrageenans, agarans and âdl-hybridâ) and minerals. These compounds have been shown to exert prebiotic effects, to regulate intestinal epithelial cell, macrophage and lymphocyte proliferation and differentiation and to modulate the immune response. Molecular mechanisms of action of peptides and polysaccharides are starting to be elucidated, and evidence indicating the involvement of epidermal growth factor receptor (EGFR), insulin-like growth factor receptor (IGFR), Toll-like receptors (TLR) and signal transduction pathways mediated by protein kinase B (PKB or AKT), nuclear factor-ÎșB (NF-ÎșB) and mitogen activated protein kinases (MAPK) will also be summarized. The need for further research is clear, but in vivo experiments point to an overall antiinflammatory effect of these algae, indicating that they can reinforce membrane barrier function.This work was funded by the Ministerio de EconomĂa y Competividad (SAF2011-22922,
SAF2011-22812, BFU2014-57736-P and AGL201E-58883-R) and by Junta de AndalucĂa (CTS164,
CTS235 and CTS6736) of the Spanish Government, and by a grant from the Argentinian Government
(CAI + D 2011 PI 0292 LI). CIBERehd is funded by the Instituto de Salud Carlos III (Spain)
Green Alga Ulva spp. Hydrolysates and Their Peptide Fractions Regulate Cytokine Production in Splenic Macrophages and Lymphocytes Involving the TLR4-NFÎșB/MAPK Pathways.
Hydrolysates of food protein sources have immunomodulatory effects, which are of interest for use as functional foods. In this study, we have characterized the immune regulatory effect on rat splenocytes, macrophages and T lymphocytes of Ulva spp. hydrolysates and their peptide fractions with or without in vitro gastrointestinal digestion and/or ultrafiltration. IL-10 was induced in almost all conditions and cell types obtained from wild type animals. The induction was in general increased by ultrafiltration and in vitro gastrointestinal digestion. TNF was also induced in basal conditions. In turn, TNF and IFN-Îł production was attenuated by the hydrolysate products in lipopolysaccharide or concanavalin A immune stimulated cells. Inhibitors for the activation of NFÎșB, MAPK p38 and JNK inhibited IL-10 induction in rat splenocytes. The response was dramatically attenuated in TLR4-/- cells, and only modestly in TLR2-/- cells. Food peptides from Ulva spp. genus exert anti-inflammatory effects in immune cells mediated by TLR4 and NFÎșB. Similarity with the immunomodulatory profile of protein hydrolysates from other sources suggests a common mechanism
Low level of red seaweed Pyropia columbina added to extruded maize products promotes colonic and systemic antioxidant environment in growing Wistar rats
The aim of this work was to evaluate the effect of consumption of extruded maize product added with a low level of the red seaweed Pyropia columbina on colonic and systemic oxidative status using a growing Wistar rat model. Twenty-four (n = 24) male Wistar rats were fed for 60 days with control (C), extruded maize product group (M), or extruded maize product added with red seaweed P. columbina (MP) diets. Rats fed whit MP showed higher catalase (CAT) and glutathione reductase (GR) colon expression than those fed with M or C. Beneficial effects on intestinal mucosal barrier function were observed, which was manifested in decrease of cecal pH (10%) and mucinase activity (67%) and increase of sIgA content (53%). Rats fed with MP diet showed lower cyclooxygenase-2 (COX-2; 43%), inducible nitric oxide synthase (iNOS; 49%), and NF-ĂȘĂą transcription factor (27%) expression in distal colon than those fed with M or C diets. Also, MP diet exerted a significant antioxidant effect on the serum and liver, increasing hepatic redox index, CAT, and GR activity. Apparent calcium absorption, total skeleton bone mineral content, and bone mineral density of total body were the same among groups. The type of dietary fiber and phenolic compounds from P. columbina could promote antioxidant environment in growing Wistar rats.Fil: Cian, RaĂșl Esteban. Universidad Nacional del Litoral. Facultad de IngenierĂa QuĂmica. Instituto de TecnologĂa de los Alimentos; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe; ArgentinaFil: Llopart, Emilce Elina. Universidad Nacional del Litoral. Facultad de IngenierĂa QuĂmica. Instituto de TecnologĂa de los Alimentos; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe; ArgentinaFil: Lopez-Oliva Muñoz, MarĂa E.. Universidad Complutense de Madrid; EspañaFil: Weisstaub, Adriana Ruth. Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica; ArgentinaFil: Zuleta, Angela. Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica; ArgentinaFil: Drago, Silvina Rosa. Universidad Nacional del Litoral. Facultad de IngenierĂa QuĂmica. Instituto de TecnologĂa de los Alimentos; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe; Argentin
Green Alga Ulva spp. Hydrolysates and Their Peptide Fractions Regulate Cytokine Production in Splenic Macrophages and Lymphocytes Involving the TLR4-NFÎșB/MAPK Pathways
Hydrolysates of food protein sources have immunomodulatory effects, which are of interest for use as functional foods. In this study, we have characterized the immune regulatory effect on rat splenocytes, macrophages and T lymphocytes of Ulva spp. hydrolysates and their peptide fractions with or without in vitro gastrointestinal digestion and/or ultrafiltration. IL-10 was induced in almost all conditions and cell types obtained from wild type animals. The induction was in general increased by ultrafiltration and in vitro gastrointestinal digestion. TNF was also induced in basal conditions. In turn, TNF and IFN-γ production was attenuated by the hydrolysate products in lipopolysaccharide or concanavalin A immune stimulated cells. Inhibitors for the activation of NFκB, MAPK p38 and JNK inhibited IL-10 induction in rat splenocytes. The response was dramatically attenuated in TLR4−/− cells, and only modestly in TLR2−/− cells. Food peptides from Ulva spp. genus exert anti-inflammatory effects in immune cells mediated by TLR4 and NFκB. Similarity with the immunomodulatory profile of protein hydrolysates from other sources suggests a common mechanism