Vehicle Accident Databases: Correctness Checks for Accident Kinematic Data

(1) Background: Data collection procedures allow to obtain harmonization of in-depth road accident databases. Plausibility of calculable accident-related kinematic parameters depends on the constraints imposed on calculation, making their uncertainty degree higher than the one for measurable parameters (i.e., traces, airbag activation, etc.). Uncertainty translates in information loss, making the statistics based on databases analysis less consistent. Since kinematic parameters describe the global accident dynamics, their correctness assessment has a fundamental importance; (2) Methods: the paper takes as reference data collected in the Initiative for the GLobal harmonisation of Accident Data (IGLAD) database for vehicle-to-vehicle crashes. The procedure, however, has general nature and applies identically for other databases and multiple impacts between vehicles. To highlight issues which can arise in accident-related data collection, 3 different checks are proposed for parameters correctness assessment; (3) Results: by 4 examples, 1 with correct and 3 with incorrect parameters reported, the paper demonstrates that errors can go beyond simple calculation uncertainty, implying that a deeper analysis is desirable in data collection; (4) Conclusions: the step-by-step guidelines described in this paper will help in increasing goodness of collected data, providing for a methodology which can be used by each individual involved in accident data collection, both for collection itself and subsequent verification analysis

Effect of Unloading Condition on the Healing Process and Effectiveness of Platelet Rich Plasma as a Countermeasure: Study on In Vivo and In Vitro Wound Healing Models

Wound healing is a very complex process that allows organisms to survive injuries. It is strictly regulated by a number of biochemical and physical factors, mechanical forces included. Studying wound healing in space is interesting for two main reasons: (i) defining tools, procedures, and protocols to manage serious wounds and burns eventually occurring in future long-lasting space exploration missions, without the possibility of timely medical evacuation to Earth; (ii) understanding the role of gravity and mechanical factors in the healing process and scarring, thus contributing to unravelling the mechanisms underlying the switching between perfect regeneration and imperfect repair with scarring. In the study presented here, a new in vivo sutured wound healing model in the leech (Hirudo medicinalis) has been used to evaluate the effect of unloading conditions on the healing process and the effectiveness of platelet rich plasma (PRP) as a countermeasure. The results reveal that microgravity caused a healing delay and structural alterations in the repair tissue, which were prevented by PRP treatment. Moreover, investigating the effects of microgravity and PRP on an in vitro wound healing model, it was found that PRP is able to counteract the microgravity-induced impairment in fibroblast migration to the wound site. This could be one of the mechanisms underlying the effectiveness of PRP in preventing healing impairment in unloading conditions

Effect of NIR laser therapy by MLS-MiS source against neuropathic pain in rats: in vivo and ex vivo analysis.

Neuropathic pain is characterized by an uncertain etiology and by a poor response to common therapies. The ineffectiveness and the frequent side effects of the drugs used to counteract neuropathic pain call for the discovery of new therapeutic strategies. Laser therapy proved to be effective for reducing pain sensitivity thus improving the quality of life. However, its application parameters and efficacy in chronic pain must be further analyzed. We investigated the pain relieving and protective effect of Photobiomodulation Therapy in a rat model of compressive mononeuropathy induced by Chronic Constriction Injury of the sciatic nerve (CCI). Laser (MLS-MiS) applications started 7 days after surgery and were performed ten times over a three week period showing a reduction in mechanical hypersensitivity and spontaneous pain that started from the first laser treatment until the end of the experiment. The ex vivo analysis highlighted the protective role of laser through the myelin sheath recovery in the sciatic nerve, inhibition of iNOS expression and enhancement of EAAT-2 levels in the spinal cord. In conclusion, this study supports laser treatment as a future therapeutic strategy in patients suffering from neuropathic pain induced by trauma