Ensiling Characteristics and Ruminal Degradation of Italian Ryegrass with or without Wilting and Added Cell Wall Degrading Enzymes

The previous experiment (Yu zhu et al.,1999) has shown that the efficacy of added enzymes varied greatly according to the DM content of the material crop. The silage DM did not alter the effects of enzymes on the in vitro digestion of NDF (Yu zhu et al.,1999, Yu zhu et al.,2000). The aim of this experiment was to study the effect of wilting and enzymes on fermentation quality, chemical composition and in situ digestion of Italian ryegrass (Lolium multiflorum Lam.) silage

Plugging Behavior of Polymer Gel in Fractures by Multi-Slug Injection

In order to understand the plugging mechanism, experiments of multi-slug gel plugging in single fracture and multiple fractures system were simulated by indoor visual fracture models. The experiments showed that there were two stages in the process of multi-slug gel plugging in single fracture: gel filling stage and gel compaction stage. When the total injection of gel is consistent, the brine flooding pressure gradient after multi-slug gel injection increased more than 2 times than the pressure gradient after single slug gel continuous injection. While there were three stages in the process of multi-slug gel plugging in multiple fractures system: Gel selectively entrance stage, gel filling stage and gel compaction stage. However, there would be no gel compaction stage, even no gel filling stage in the process of single cycle gel plugging in multiple fractures system.Key words: Fractured reservoir; Multi-slug; Polymer gel; Plugging proces

A Drought-Inducible Transcription Factor Delays Reproductive Timing in Rice

The molecular mechanisms underlying photoperiod or temperature control of flowering time have been recently elucidated, but how plants regulate flowering time in response to other external factors, such as water availability, remains poorly understood. Using a large-scale Hybrid Transcription Factor approach, we identified a bZIP transcriptional factor, O. sativa ABA responsive element binding factor 1 (OsABF1), which acts as a suppressor of floral transition in a photoperiod-independent manner. Simultaneous knockdown of both OsABF1 and its closest homologous gene, OsbZIP40, in rice (Oryza sativa) by RNA interference results in a significantly earlier flowering phenotype. Molecular and genetic analyses demonstrate that a drought regime enhances expression of the OsABF1 gene, which indirectly suppresses expression of the Early heading date 1 (Ehd1) gene that encodes a key activator of rice flowering. Furthermore, we identified a drought-inducible gene named OsWRKY104 that is under the direct regulation of OsABF1. Overexpression of OsWRKY104 can suppress Ehd1 expression and confers a later flowering phenotype in rice. Together, these findings reveal a novel pathway by which rice modulates heading date in response to the change of ambient water availability

Effects of Bacillus subtilis or Lentilactobacillus buchneri on aerobic stability, and the microbial community in aerobic exposure of whole plant corn silage

This study aimed to evaluate the effects of Bacillus subtilis or Lentilactobacillus buchneri on the fermentation quality, aerobic stability, and bacterial and fungal communities of whole plant corn silage during aerobic exposure. Whole plant corn was harvested at the wax maturity stage, which chopped to a length of approximately 1 cm, and treated with the following: distilled sterile water control, 2.0 × 105 CFU/g of Lentilactobacillus buchneri (LB) or 2.0 × 105 CFU/g of Bacillus subtilis (BS) for 42 days silage. Then, the samples were exposed to air (23–28°C) after opening and sampled at 0, 18 and 60 h, to investigate fermentation quality, bacterial and fungal communities, and aerobic stability. Inoculation with LB or BS increased the pH value, acetic acid, and ammonia nitrogen content of silage (P < 0.05), but it was still far below the threshold of inferior silage, the yield of ethanol was reduced (P < 0.05), and satisfactory fermentation quality was achieved. With the extension of the aerobic exposure time, inoculation with LB or BS prolonged the aerobic stabilization time of silage, attenuated the trend of pH increase during aerobic exposure, and increased the residues of lactic acid and acetic acid. The bacterial and fungal alpha diversity indices gradually declined, and the relative abundance of Basidiomycota and Kazachstania gradually increased. The relative abundance of Weissella and unclassified_f_Enterobacteria was higher and the relative abundance of Kazachstania was lower after inoculation with BS compared to the CK group. According to the correlation analysis, Bacillus and Kazachstania are bacteria and fungi that are more closely related to aerobic spoilage and inoculation with LB or BS could inhibit spoilage. The FUNGuild predictive analysis indicated that the higher relative abundance of fungal parasite-undefined saprotroph in the LB or BS groups at AS2, may account for its good aerobic stability. In conclusion, silage inoculated with LB or BS had better fermentation quality and improved aerobic stability by effectively inhibiting the microorganisms that induce aerobic spoilage