Regulation of Xenopus oocyte meiosis arrest by G protein βγ subunits

AbstractBackground: Progesterone induces the resumption of meiosis (maturation) in Xenopus oocytes through a nongenomic mechanism involving inhibition of an oocyte adenylyl cyclase and reduction of intracellular cAMP. However, progesterone action in Xenopus oocytes is not blocked by pertussis toxin, and this finding indicates that the inhibition of the oocyte adenylyl cyclase is not mediated by the α subunits of classical Gi-type G proteins.Results: To investigate the possibility that G protein βγ subunits, rather than α subunits, play a key role in regulating oocyte maturation, we have employed two structurally distinct G protein βγ scavengers (Gtα and βARK-CCAAX) to sequester free Gβγ dimers. We demonstrated that the injection of mRNA encoding either of these Gβγ scavengers induced oocyte maturation. The Gβγ scavengers bound an endogenous, membrane-associated Gβ subunit, indistinguishable from Xenopus Gβ1 derived from mRNA injection. The injection of Xenopus Gβ1 mRNA, together with bovine Gγ2 mRNA, elevated oocyte cAMP levels and inhibited progesterone-induced oocyte maturation.Conclusion: An endogenous G protein βγ dimer, likely including Xenopus Gβ1, is responsible for maintaining oocyte meiosis arrest. Resumption of meiosis is induced by Gβγ scavengers in vitro or, naturally, by progesterone via a mechanism that suppresses the release of Gβγ

Microwave-assisted Acid-catalyzed Hydrolysis of Hemicelluloses in Rice Husk into Xylose

The development of an environmentally benign process for the hydrolysis of hemicelluloses into xylose could be one of the key technologies for making full use of biomass in the future. This paper studies dilute acid hydrolysis of hemicelluloses in rice husk (RH) into xylose using microwave radiation. Fourier transform infrared-attenuated total reflection spectroscopy was employed to quantitatively analyze xylose. The influences of hydrolysis parameters such as temperature, time, acid concentration, and ratio of RH to sulfuric acid on the yield of xylose in acid hydrolysis of RH were also investigated. The optimum hydrolysis conditions of hemicelluloses in RH to xylose are as follows: 4 wt% of H2SO4 concentration, 150 °C hydrolysis temperature, 25 min reaction time, and 1:7 ratio of RH (g) to H2SO4 (mL). Under optimum hydrolysis conditions, a yield of 32.96% xylose is obtained

C500 variants conveying complete mucosal immunity against fatal infections of pigs with Salmonella enterica serovar Choleraesuis C78-1 or F18+ Shiga toxin-producing Escherichia coli

Salmonella enterica serovar Choleraesuis (S. Choleraesuis) C500 strain is a live, attenuated vaccine strain that has been used in China for over 40 years to prevent piglet paratyphoid. However, this vaccine is limited by its toxicity and does not offer protection against diseases caused by F18+ Shiga toxin-producing Escherichia coli (STEC), which accounts for substantial economic losses in the swine industry. We recently generated a less toxic derivative of C500 strain with both asd and crp deletion (S. Choleraesuis C520) and assessed its efficacy in mice. In addition, we demonstrate that C520 is also less toxic in pigs and is effective in protecting pigs against S. Choleraesuis when administered orally. To develop a vaccine with a broader range of protection, we prepared a variant of C520 (S. Choleraesuis C522), which expresses rSF, a fusion protein comprised of the fimbriae adhesin domain FedF and the Shiga toxin-producing IIe B domain antigen. For comparison, we also prepared a control vector strain (S. Choleraesuis C521). After oral vaccination of pigs, these strains contributed to persistent colonization of the intestinal mucosa and lymphoid tissues and elicited both cytokine expression and humoral immune responses. Furthermore, oral immunization with C522 elicited both S. Choleraesuis and rSF-specific immunoglobulin G (IgG) and IgA antibodies in the sera and gut mucosa, respectively. To further evaluate the feasibility and efficacy of these strains as mucosal delivery vectors via oral vaccination, we evaluated their protective efficacy against fatal infection with S. Choleraesuis C78-1, as well as the F18+ Shiga toxin-producing Escherichia coli field strain Ee, which elicits acute edema disease. C521 conferred complete protection against fatal infection with C78-1; and C522 conferred complete protection against fatal infection with both C78-1 and Ee. Our results suggest that C520, C521, and C522 are competent to provide complete mucosal immune protection against fatal infection with S. Choleraesuis in swine and that C522 equally qualifies as an oral vaccine vector for protection against F18+ Shiga toxin-producing Escherichia coli

Engineering band structures and topological invariants by transformation optics

By introducing the transformation optics method to periodic systems, we show the tunability of the band structures by comparing the results from original spaces and transformed spaces. Interestingly, we find the topological invariant Chern number will change sign when the orientation of the Brillouin zone flipped. The new platform we provided for engineering the band diagram and topological invariant might lead to the development of both transformation optics and photonic topological states.Comment: 6 pages, 3 figure

Positive association between blood ethylene oxide levels and metabolic syndrome: NHANES 2013-2020

PurposeThe exposure of Ethylene oxide (EO) is linked to systemic inflammatory response and various cardiovascular risk factors. Hemoglobin’s binding to ethylene oxide (HbEO) was used to measure serum EO level. This research aims to explore the association between metabolic syndrome (MetS) and HbEO, and between HbEO and components of metabolic syndrome.MethodThis research included 1842 participants from 2013 to 2020 in National Health and Nutrition Examination Survey (NHANES) database. Weighted logistic regression models were used to analyze the relationship between HbEO and metabolic syndrome risk, using odds ratio (OR) and 95% confidence interval (CI). The restricted cubic spline plot explores whether there is a dose-response relationship between HbEO and MetS risk. Subgroup analysis was performed to analyze study heterogeneity.ResultsSignificant differences were found in gender, educational level, marital status, diabetes status and hypertension among different groups (P < 0.001, P = 0.007, P = 0.003, P < 0.001, P < 0.001, respectively). The serum HbEO level exhibited positive correlation with metabolic syndrome risk in Q2 level (OR=1.64, 1.04~2.48), Q3 level (OR=1.99, 1.29~3.08), and Q4 level (OR=2.89, 1.92~4.34). The dose-response association suggested a possible linear association between serum HbEO and metabolic syndrome risk (P-overall=0.0359, P-non-linear=0.179). L-shaped association was found between HbEO and the risk of MetS in female population, obese population and mid-age and elder population (P-overall<0.001, P-non-linear=0.0024; P-overall=0.0107, P-non-linear=0.0055 P-overall<0.001 P-non-linear=0.0157).ConclusionThis study indicates a linear correlation between MetS and HbEO, with MetS risk escalating as HbEO levels increase. The prevalence of MetS varies depending on BMI, age and gender, and these factors can also influence MetS prevalence when exposed to EO

Chronic Alcohol Causes Alteration of Lipidome Profiling in Brain

Much efforts have been tried to clarify the molecular mechanism of alcohol-induced brain damage from the perspective of genome and protein; however, the effect of chronic alcohol exposure on global lipid profiling of brain is unclear. In the present study, by using Q-TOF/MS-based lipidomics approach, we investigated the comprehensive lipidome profiling of brain from the rats orally administrated with alcohol daily, continuously for one year. Through systematically analysis of all lipids in prefrontal cortex (PFC) and striatum region, we found that long-term alcohol exposure profoundly modified brain lipidome profiling. Notably, three kinds of lipid classes, glycerophospholipid (GP), glycerolipid (GL) and fatty acyls (FA), were significantly increased in these two brain regions. Interestingly, most of the modified lipids were involved in synthetic pathways of endoplasmic reticulum (ER), which may result in ER stress-related metabolic disruption. Moreover, alcohol-modified lipid species displayed long length of carbon chain with high degree of unsaturation. Taken together, our results firstly present that chronic alcohol exposure markedly modifies brain lipidomic profiling, which may activate ER stress and eventually result in neurotoxicity. These findings provide a new insight into the mechanism of alcohol-related brain damage.Peer reviewe

Acidification oxidation reagent system optimization on coal seams and stimulation effect evaluation

China has abundant coalbed methane (CBM) resources, and most of them are low-permeability and tight reservoirs, with generally low production rate and small recovery factor. Existing technologies face great challenges to meet the demand on CBM in China. It is desirable to develop new methods to improve the production rate and enhance recovery factor. In addition to physical stimulation methods such as hydraulic fracturing and open-hole cave completion, the use of chemical methods to improve physical properties of coal reservoirs has also been a hot research topic in recent years. Coal reservoir acidification and oxidation technology can promote desorption of gas and enlarge permeability of reservoir. But for different coal rank coal reservoirs, the acidification and oxidation agents need to be optimized and their performance evaluated. Laboratory experiments are conducted to compare and analyze the physical properties coal samples from Baode, Mu’ai, and Xinjiang blocks, including coal rank, texture, macroscopic characteristics, quality, porosity, permeability, element, and mineral composition. The optimal concentration of hydrochloric acid is determined through pre-dissolution experiment of coal powder in acid solution. Then a five-factor and three-level orthogonal experiment for acid solution optimization is designed and performed by using Design-Expert software, which identifies the sensitive factors affecting the dissolution. For the coal samples in Baode, Mu’ai, and Xinjiang blocks, the oxidant types and the corresponding acidification and oxidation agent systems are optimized. Applying these acidification and oxidation agent systems to coal samples from Baode, Mu’ai, and Xinjiang blocks, the change of porosity, permeability, and wettability are compared and analyzed. Finally, through numerical simulation, the gas production is predicted for acidification and oxidation in typical well group in Block Mu’ai. Results show that the acid solution has the best dissolution at a concentration of hydrochloric acid of 3 mol/L to 4 mol/L; Top factors played in the experiment are soaking time, acid type, soaking temperature, coal sample type, and acid concentration, in descending order of importance; The optimal oxidant is a hydrogen peroxide solution with a concentration of 3%; the mixed acidification oxidant formula in Baode block is 10% HCl + 2% CH3COOH + 2% HF + 3% H2O2; The optimal mixed acidification oxidant formula in Mu’ai block is 8% HCl + 2% CH3COOH + 4% HF + 3% H2O2; the optimal mixed acidification oxidant formula in Xinjiang block is 12% HCl + 1% CH3COOH + 1% HF + 3% H2O2; The higher the coal rank, the greater the HF content in the optimal acidification oxidant system. Both acidification and oxidation improve the porosity and permeability of coal samples to some extent, and the improvement in low-rank coal is more significant than that in high-rank coal. Acidification and oxidation have different effects on the wettability of coal: Acidification increases the hydrophilicity of coal, whereas oxidation reduce the hydrophilicity of coal; and the hydrophilicity of coal samples treated by the optimized acidification and oxidation system is weakened. Reservoir simulation results show that acidification and oxidation lead to a recovery factor of 64.64% after 10 years of production, which is 19.72% higher than that without acidification and oxidation. The advantage of acidification and oxidation is 0.97% after 18 years of production. However, the acidification and oxidation saved 8 years of production time to achieve a close final recovery factor, which greatly reduces the operating costs. The optimized acidizing oxidation agent systems for CBM reservoirs with low, medium, and high ranks improved the desorption and permeability of the target reservoirs, and increase well production and recovery factor. This research provides technical support for stimulation practices of CBM reservoirs in the aforementioned blocks in China, as well as similar coal reservoirs in the world

Comparison of microbiota structure in reproductive tract of Yanbian cattle and Yanhuang cattle

Microbiota in the reproductive tract of cattle play a vital role in maintaining normal reproduction. However, the information on microbiota in different parts of reproductive tracts with different genetic background is few. The aim of the present study was to describe and compare the microbiota in vagina, cervix and uterus of Yanbian cattle and Yanhuang cattle. The results showed that microbial diversity increases from the vagina to the uterus. The top three bacterial phyla in bovine reproductive tract were Proteobacteria, Firmicutes and Bacteroidetes, accounting for more than 85%. From the vagina to the uterus, the relative abundance of Proteobacteria gradually decreased, while that of Firmicutes gradually increased. Phylum-level Firmicutes and genus-level UCG_010 were significantly enriched in the uterus of Yanbian cattle and Yanhuang cattle. Comparing the same parts of the two breeds, it was found that there was no significant difference in alpha diversity, but significant differences in beta diversity. In addition, microbiota with significant differences in the relative abundance of the reproductive tract were found. These findings lay a foundation for a comprehensive understanding of the structure of the genital tract microbiota of cows and its regulatory mechanisms