2,764 research outputs found
Performance of an Anode Supported Solid Oxide Fuel Cell with Indirect Internal Reforming
The conversion of fuel into hydrogen-rich gas is necessary for fuel cells. This can be achieved either indirectly in fuel processing systems, in which the hydrocarbon feed is converted in an external catalytic steam reformer, or directly in the fuel cell. In this paper, the unit module of solid oxide fuel cell was assembled by one reformer and four cells. The reformer was fabricated by extruded dummy cell and combined with two cells on each side respectively. The reforming catalyst was coated on internal channel of the dummy cell. The unit module has successfully tested with wet CH4 as fuel and air as oxidant and its maximum power density exceeded 150mW/cm(2) at 750 degrees C.open110Nsciescopu
The role of fusogenic vesicles in the regulation of nuclear envelope assembly
Earlier studies of the male pronucleus (MPN) formation in echinoderms have isolated fusogenic vesicles, known as MV1, essential for nuclear envelope (NE) assembly. MV1 is a membrane compartment with elevated levels of phosphoinositides, a lipid-modifying enzyme PLCγ and its upstream regulator SFK1. These are responsible for production of localised diacylglycerol (DAG) which facilitates membrane fusion. The fusion mechanism in mammalian NE assembly is uncharacterised. We hypothesise MV1-like vesicles are responsible for completing the NE in mammalian cells by sealing the NE gaps at telophase. To study the fusion mechanism in mammalian NE assembly, subcellular fractions analogous to MV1 were isolated from CHO and HeLa cells. However, these membrane fractions were rarely separated from the ER, Golgi and endosomes subfractions. Electron tomography confirmed the presence of membrane vesicles (MVs) in the vicinity of the NE gaps at telophase. Moreover, the quantity of these MVs was significantly higher in the regions of large gap versus regions without gap, suggesting a putative consumption of these MVs for gap closure. Furthermore, PtdIns(3,4,5)P3 and class I PI3 kinase were detected in endomembranes at the vicinity of the mammalian NE implying the presence of MV1-like vesicles. DAG-enriched MVs were detected in interphase cells using a miniSOG-conjugated C1aC1b domain; they may be involved in house-keeping fusion events. Using a chemically-induced dimerisation system and correlative light and electron microscopy, localised DAG production was demonstrated to be essential for mammalian NE assembly. Acute DAG depletion in the NE resulted in NE fragmentation. Fragmented NE was rescued by addition of exogenous DAG. Also, we show for the first time that Golgi-DAG depletion led to fragmented NE, signifying that the Golgi may produce fusogenic vesicles. Our findings show that the regulatory mechanisms involved in the MPN formation in fertilised echinoderm eggs are also conserved in mammalian NE assembly during mitosis
Intelligent spider for Internet searching
As World Wide Web (WWW) based Internet services become more popular, information overload also becomes a pressing research problem. Difficulties with searching on the Internet get worse as the amount of information that is available increases. A scalable approach to support Internet search is critical to the success of Internet services and other current or future national information infrastructure (NII) applications. A new approach to build an intelligent personal spider (agent), which is based on automatic textual analysis of Internet documents, is proposed. Best first search and genetic algorithm have been tested to develop the intelligent spider. These personal spiders are able to dynamically and intelligently analyze the contents of the users' selected homepages as the starting point to search for the most relevant homepages based on the links and indexing. An intelligent spider must have the capability to make adjustments according to progress of searching in order to be an intelligent agent. However, the current searching engines do not have communication between the users and the robots. The spider presented in the paper uses Java to develop the user interface such that the users can adjust the control parameters according to the progress and observe the intermediate results. The performances of the genetic algorithm based and best first search based spiders are also reported.published_or_final_versio
Partial suppression of a strongly expressed tonoplast sucrose transporter affects water use and carbon partitioning in Populus
Designing CALL for learning Chinese characters
Despite the enormity of its quantity, printed or written forms of Chinese characters are composed from a limited number of common components. For example, the characters for pond( ), lake( ), stream( ), river ( ), sea( ) and ocean( ) all contain a component in common, a three-dot component representing water. When this clue is explicitly highlighted to students, the learning of Chinese characters can be greatly enhanced. Using a computer to help students to develop this kind of structural awareness about language learning has not yet been thoroughly examined. This paper reports on the design of CALL software based on a pedagogic method which helps students to develop the higher order skills to analyse and categorise Chinese characters by using components. The result of the classroom experiment has shown supportive evidence on the feasibility and the need of integrating the software with an affective and contextual way of teaching Chinese characters.postprin
POCS-based reconstruction of multiplexed sensitivity encoded MRI (POCSMUSE): A general algorithm for reducing motion-related artifacts.
PURPOSE: A projection onto convex sets reconstruction of multiplexed sensitivity encoded MRI (POCSMUSE) is developed to reduce motion-related artifacts, including respiration artifacts in abdominal imaging and aliasing artifacts in interleaved diffusion-weighted imaging. THEORY: Images with reduced artifacts are reconstructed with an iterative projection onto convex sets (POCS) procedure that uses the coil sensitivity profile as a constraint. This method can be applied to data obtained with different pulse sequences and k-space trajectories. In addition, various constraints can be incorporated to stabilize the reconstruction of ill-conditioned matrices. METHODS: The POCSMUSE technique was applied to abdominal fast spin-echo imaging data, and its effectiveness in respiratory-triggered scans was evaluated. The POCSMUSE method was also applied to reduce aliasing artifacts due to shot-to-shot phase variations in interleaved diffusion-weighted imaging data corresponding to different k-space trajectories and matrix condition numbers. RESULTS: Experimental results show that the POCSMUSE technique can effectively reduce motion-related artifacts in data obtained with different pulse sequences, k-space trajectories and contrasts. CONCLUSION: POCSMUSE is a general post-processing algorithm for reduction of motion-related artifacts. It is compatible with different pulse sequences, and can also be used to further reduce residual artifacts in data produced by existing motion artifact reduction methods
Chemical ordering in PtNi nanocrystals
We investigated the chemical ordering in PtNi nanocrystals fabricated on sapphire substrate using in-situ synchrotron X-ray scattering. Nanocrystals with composition close to 1:1 were ordered in the tetragonal L1(0) structure at low temperatures. The transition to disordered FCC structure occurred at around 640 degrees C and substantial hysteresis of about 50 K was observed. Nanocrystals of smaller sizes fabricated under the same conditions were Ni rich and ordered into Cu3Au type L1(2) structure. Significantly higher degree of chemical ordering was observed in L1(2) structure than in L1(0) structure. (C) 2016 Elsevier B.V. All rights reserved.1144Ysciescopu
Soil-Structure Interaction on the Response of Jacket Type Offshore Wind Turbine
Jacket structures are still at the early stage of their development for use in the offshore wind industry. The aim of this paper is to investigate the effect of the soil-structure interaction on the response of an offshore wind turbine with a jacket-type foundation. For this purpose, two different models of flexible foundation-the p-y model and the p-y model considering pile groups effect-are employed to compare the dynamic responses with the fixed-base model. The modal analysis and the coupled dynamic analysis are carried out under deterministic and stochastic conditions. The influence of the soil-structure interaction on the response of the jacket foundation predicts that the flexible foundation model is necessary to estimate the loads of the offshore wind turbine structure well. It is suggested that during fatigue analysis the pile group effect should be considered for the jacket foundation.None1174Ysciescopu
Tricalbin proteins regulate plasma membrane phospholipid homeostasis
The evolutionarily conserved extended synaptotagmin (E-Syt) proteins are calcium-activated lipid transfer proteins that function at contacts between the ER and plasma membrane (ER-PM contacts). However, roles of the E-Syt family members in PM lipid organisation remain incomplete. Among the E-Syt family, the yeast tricalbin (Tcb) proteins are essential for PM integrity upon heat stress, but it is not known how they contribute to PM maintenance. Using quantitative lipidomics and microscopy, we find that the Tcb proteins regulate phosphatidylserine homeostasis at the PM. Moreover, upon heat-induced membrane stress, Tcb3 co-localises with the PM protein Sfk1 that is implicated in PM phospholipid asymmetry and integrity. The Tcb proteins also control the PM targeting of the known phosphatidylserine effector Pkc1 upon heat-induced stress. Phosphatidylserine has evolutionarily conserved roles in PM organisation, integrity, and repair. We propose that phospholipid regulation is an ancient essential function of E-Syt family members required for PM integrity
Cyclic deformation characteristics of S355 and S690 steels under different loading protocols
Despite of excellent high strength to self-weight ratios of the S690 steels, when compared with the S355 steels, there is a widespread concern regarding the ductility of the S690 steels. It is generally considered that the ductility of the S690 steels is significantly lower than that of the S355 steels – this is the general understandings the authors attempt to investigate. This paper presents an experimental investigation into cyclic deformation characteristics of both S355 and S690 steels through low-cycle high-strain cyclic tests with two different loading protocols. A detailed account of the results of 32 cyclic tests on both the S355 and the S690 funnel-shaped coupons is presented. Effects of four different target strains and two different loading frequencies are also examined in details. For the ranges of loading protocols, strain amplitudes, and frequencies considered, the hysteretic responses of these coupons of the two steels are compared directly in terms of engineering stress–strain curves based on their nominal diameters. Microstructures of the fractured coupons of the two steels are also identified for comparison. Contrary to the general understandings, it is demonstrated that the high strength S690 steels do have a good ductility under both monotonic and cyclic actions. Moreover, depending on specific loading protocols and target strains, the cyclic deformation characteristics of the S690 steels are demonstrated to be superior to those of the S355 steels in terms of the number of cycles completed prior to failure and their corresponding energy dissipation characteristic under various target strains up to ±10.0%. The findings of this experimental investigation highlight the importance of establishing ductility requirements and cyclic deformation characteristics for the high strength S690 steels in accordance with specifically designed cyclic tests rather than relying solely on conventional monotonic tensile tests
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