Non-invasive digital etching of van der Waals semiconductors

The capability to finely tailor material thickness with simultaneous atomic precision and non-invasivity would be useful for constructing quantum platforms and post-Moore microelectronics. However, it remains challenging to attain synchronized controls over tailoring selectivity and precision. Here we report a protocol that allows for non-invasive and atomically digital etching of van der Waals transition-metal dichalcogenides through selective alloying via low-temperature thermal diffusion and subsequent wet etching. The mechanism of selective alloying between sacrifice metal atoms and defective or pristine dichalcogenides is analyzed with high-resolution scanning transmission electron microscopy. Also, the non-invasive nature and atomic level precision of our etching technique are corroborated by consistent spectral, crystallographic and electrical characterization measurements. The low-temperature charge mobility of as-etched MoS$_2$ reaches up to $1200\,$cm$^{2}\cdot$V$^{-1}\cdot$s$^{-1}$, comparable to that of exfoliated pristine counterparts. The entire protocol represents a highly precise and non-invasive tailoring route for material manipulation.Comment: 46 pages, 4 figures, with S

Non-invasive digital etching of van der Waals semiconductors

The capability to finely tailor material thickness with simultaneous atomic precision and non-invasivity would be useful for constructing quantum platforms and post-Moore microelectronics. However, it remains challenging to attain synchronized controls over tailoring selectivity and precision. Here we report a protocol that allows for non-invasive and atomically digital etching of van der Waals transition-metal dichalcogenides through selective alloying via low-temperature thermal diffusion and subsequent wet etching. The mechanism of selective alloying between sacrifice metal atoms and defective or pristine dichalcogenides is analyzed with high-resolution scanning transmission electron microscopy. Also, the non-invasive nature and atomic level precision of our etching technique are corroborated by consistent spectral, crystallographic, and electrical characterization measurements. The low-temperature charge mobility of as-etched MoS2 reaches up to 1200 cm2 V−1s−1, comparable to that of exfoliated pristine counterparts. The entire protocol represents a highly precise and non-invasive tailoring route for material manipulation

From probiotics to postbiotics: Concepts and applications

Abstract In recent years, the important role of gut microbiota in promoting animal health and regulating immune function in livestock and poultry has been widely reported. The issue of animal health problems causes significant economic losses each year. Probiotics and postbiotics have been widely developed as additives due to their beneficial effects in balancing host gut microbiota, enhancing intestinal epithelial barrier, regulating immunity, and whole‐body metabolism. Probiotics and postbiotics are composed of complex ingredients, with different components and compositions having different effects, requiring classification for discussing their mechanisms of action. Probiotics and postbiotics have considerable prospects in preventing various diseases in the livestock industry and animal feed and medical applications. This review highlights the application value of probiotics and postbiotics as potential probiotic products, emphasizing their concept, mechanism of action, and application, to improve the productivity of livestock and poultry

Experimental and Theoretical Investigations on Diamond Wire Sawing for a NdFeB Magnet

The normal processing of sintered NdFeB magnets, used in many applied fields, involves diamond wire sawing. Due to the fact of its relatively lower hardness and high brittleness, the surface roughness and periodic waviness of the sawed surface have become a serious problem, but the surface formation mechanism is still unknown. In this work, a diamond wire sawing experiment with a NdFeB magnet was conducted while both the cutting force and the diamond wire lateral displacement were monitored. The vibration, the lateral swing of the wire and the cutting force were thoroughly analyzed. After the experiment, the surface morphology was carefully inspected under both a white light interferometer and SEM. It was discovered that the lateral swing of the diamond wire was the main cause of the periodic waviness on the surface, the PV of which was positively proportional to the normal cutting force. The surface morphology and surface roughness along the saw mark revealed that the vibration impact of ploughing/rubbing grits can induce the NdFeB grain to loosen off and cause more brittle fractures when the feed rate was 0.05 mm/min under wet cutting

A practical method utilizing multi-spectral LiDAR to aid points cloud matching in SLAM

Light Detection and Ranging (LiDAR) sensors are popular in Simultaneous Localization and Mapping (SLAM) owing to their capability of obtaining ranging information actively. Researchers have attempted to use the intensity information that accompanies each range measurement to enhance LiDAR SLAM positioning accuracy. However, before employing LiDAR intensities in SLAM, a calibration operation is usually carried out so that the intensity is independent of the incident angle and range. The range is determined from the laser beam transmitting time. Therefore, the key to using LiDAR intensities in SLAM is to obtain the incident angle between the laser beam and target surface. In a complex environment, it is difficult to obtain the incident angle robustly. This procedure also complicates the data processing in SLAM and as a result, further application of the LiDAR intensity in SLAM is hampered. Motivated by this problem, in the present study, we propose a Hyperspectral LiDAR (HSL)-based-intensity calibration-free method to aid point cloud matching in SLAM. HSL employed in this study can obtain an eight-channel range accompanied by corresponding intensity measurements. Owing to the design of the laser, the eight-channel range and intensity were collected with the same incident angle and range. According to the laser beam radiation model, the ratio values between two randomly selected channels' intensities at an identical target are independent of the range information and incident angle. To test the proposed method, the HSL was employed to scan a wall with different coloured papers pasted on it (white, red, yellow, pink, and green) at four distinct positions along a corridor (with an interval of 60 cm in between two consecutive positions). Then, a ratio value vector was constructed for each scan. The ratio value vectors between consecutive laser scans were employed to match the point cloud. A classic Iterative Closest Point (ICP) algorithm was employed to estimate the HSL motion using the range information from the matched point clouds. According to the test results, we found that pink and green papers were distinctive at 650, 690, and 720 nm. A ratio value vector was constructed using 650-nm spectral information against the reference channel. Furthermore, compared with the classic ICP using range information only, the proposed method that matched ratio value vectors presented an improved performance in heading angle estimation. For the best case in the field test, the proposed method enhanced the heading angle estimation by 72%, and showed an average 25.5% improvement in a featureless spatial testing environment. The results of the primary test indicated that the proposed method has the potential to aid point cloud matching in typical SLAM of real scenarios.Peer reviewe

The Putative Pocket Protein Binding Site of Autographa californica Nucleopolyhedrovirus BV/ODV-C42 Is Required for Virus-Induced Nuclear Actin Polymerization▿

Nuclear filamentous actin (F-actin) is essential for nucleocapsid morphogenesis of lepidopteran nucleopolyhedroviruses. Previously, we had demonstrated that Autographa californica multiple nucleopolyhedrovirus (AcMNPV) BV/ODV-C42 (C42) is involved in nuclear actin polymerization by recruiting P78/83, an AcMNPV orf9-encoded N-WASP homology protein that is capable of activating an actin-related-protein 2/3 (Arp2/3) complex to initiate actin polymerization, to the nucleus. To further investigate the role of C42 in virus-induced actin polymerization, the recombinant bacmid vAcp78/83nls-gfp, with a c42 knockout, p78/83 tagged with a nuclear localization signal coding sequence, and egfp as a reporter gene under the control of the Pp10 promoter, was constructed and transfected to Sf9 cells. In the nuclei of vAcp78/83nls-gfp-transfected cells, polymerized F-actin filaments were absent, whereas other actin polymerization elements (i.e., P78/83, G-actin, and Arp2/3 complex) were present. This in vivo evidence indicated that C42 actively participates in the nuclear actin polymerization process as a key element, besides its role in recruiting P78/83 to the nucleus. In order to collect in vitro evidence for the participation of C42 in actin polymerization, an anti-C42 antibody was used to neutralize the viral nucleocapsid, which is capable of initiating actin polymerization in vitro. Both the kinetics of pyrene-actin polymerization and F-actin-specific staining by phalloidin indicated that anti-C42 can significantly attenuate the efficiency of F-actin formation compared to that with control antibodies. Furthermore, we have identified the putative pocket protein binding sequence (PPBS) on C42 that is essential for C42 to exert its function in nuclear actin polymerization

Monitoring the Invasion of <i>S. alterniflora</i> on the Yangtze River Delta, China, Using Time Series Landsat Images during 1990–2022

Spartina alterniflora (S. alterniflora) has grown rapidly in China since its introduction in 1979, showing the trend of alien species invasion, which has seriously affected the ecosystem balance of coastal wetlands. The temporal and spatial expansion law of S. alterniflora can be obtained through remote sensing monitoring, which can provide a reference and basis for S. alterniflora management. This paper presents a method for extracting and mapping S. alterniflora based on phenological characteristics. The coastal areas of the Yangtze River Delta Urban Agglomeration are selected as the research area, and the Landsat time series data from 1990 to 2022 on the Google Earth Engine (GEE) platform are used to support the experiment in this paper. Firstly, the possible growing area of S. alterniflora was extracted using the normalized differential moisture index (NDMI), normalized differential vegetation index (NDVI), and normalized differential water index (NDWI); Then, the time series curve characterizing the phenological characteristics of vegetation was constructed using the vegetation index to determine the difference phase of phenological characteristics between S. alterniflora and other vegetation. Finally, a decision tree was constructed based on the phenological feature difference phase data to extract S. alterniflora, and it is applied to the analysis of temporal and spatial changes of S. alterniflora in the study area from 1990 to 2022. The results show that the area of S. alterniflora increased from ~1426 ha in 1990 to ~44,508 ha in 2022. However, the area of S. alterniflora began to show negative growth in 2015 due to the construction of nature reserves and ecological management. The results of correlation analysis showed that the growth of C. japonicum was significantly affected by temperature stress and weakly affected by precipitation. This study verified that Landsat time series images can effectively extract vegetation phenological information, which has strong feasibility for extraction and dynamic monitoring of S. alterniflora and provides technical support for the management and monitoring of invasive plants in coastal wetlands

Protein Inhibitor of Activated STAT2 Restricts HCV Replication by Modulating Viral Proteins Degradation

Hepatitis C virus (HCV) replication in cells is controlled by many host factors. In this report, we found that protein inhibitor of activated STAT2 (PIAS2), which is a small ubiquitin-like modifier (SUMO) E3 ligase, restricted HCV replication. During infection, HCV core, NS3 and NS5A protein expression, as well as the viral assembly and budding efficiency were enhanced when endogenous PIAS2 was knocked down, whereas exogenous PIAS2 expression decreased HCV core, NS3, and NS5A protein expression and the viral assembly and budding efficiency. PIAS2 did not influence the viral entry, RNA replication, and protein translation steps of the viral life cycle. When expressed together with SUMO1, PIAS2 reduced the HCV core, NS3 and NS5A protein levels expressed from individual plasmids through the proteasome pathway in a ubiquitin-independent manner; the stability of these proteins in the HCV infectious system was enhanced when PIAS2 was knocked down. Furthermore, we found that the core was SUMOylated at amino acid K78, and PIAS2 enhanced the SUMOylation level of the core

Additional file 1: Figure S1. of Knockdown of ST6Gal-I increases cisplatin sensitivity in cervical cancer cells

ST6Gal-I mRNA expression levels after transfection for different time points analyzed in this study. (TIF 21 kb

Revealing the Origin of Transition-Metal Migration in Layered Sodium-Ion Battery Cathodes: Random Na Extraction and Na-Free Layer Formation

Cation migration often occurs in layered oxide cathodes of lithium-ion batteries due to the similar ion radius of Li and transition metals (TMs). Although Na and TM show a big difference of ion radius, TMs in layered cathodes of sodium-ion batteries (SIBs) can still migrate to Na layer, leading to serious electrochemical degeneration. To elucidate the origin of TM migration in layered SIB cathodes, we choose NaCrO2, a typical layered cathode suffering from serious TM migration, as a model material and find that the TM migration is derived from the random desodiation and subsequent formation of Na-free layer at high charge potential. A Ru/Ti co-doping strategy is developed to address the issue, where the doped active Ru is first oxidized to create a selective desodiation and the doped inactive Ti can function as a pillar to avoid complete desodiation in Ru-contained TM layers, leading to the suppression of the Na-free layer formation and subsequent enhanced electrochemical performance