644 research outputs found
Applying Customer Knowledge Management To Alignment And Integration Of Strategy Maps
Customer knowledge refers to understanding customers’ needs, wants and aims. It is essential for an organization to align its processes, products and services to build customer relationships. Managers need to understand how the acquisition and use of customer-related knowledge create value for the organization. Customer knowledge management (CKM) is a strategy that focuses on the task of gathering information including finding effective ways to extract data from customers as well as to locate and absorb information from other sources. This paper builds an information retrieval (IR) and information extraction (IE) system to analyze customer knowledge from the Web. The system is designed to assist organizations in conducting the alignment and integration of strategy objects in strategy maps
Development of beam arrangement design for tunable diode laser absorption tomography reconstruction based on Tikhonov regularization parameter matrix
DiaquaÂbis(benzylÂoxyacetato)copper(II)
In the title mononuclear complex, [Cu(C9H9O3)2(H2O)2], the CuII ion, located on an inversion center, is hexaÂcoordinated by four O atoms from two benzylÂoxyacetate ligands [Cu—O = 1.9420 (14) and 2.2922 (14) Å] and two water molÂecules [Cu—O = 2.0157 (15) Å] in a distorted octaÂhedral geometry. In the crystal structure, interÂmolecular O—Hâ‹ŻO hydrogen bonds link the molÂecules into layers parallel to the bc plane
Helium-bearing superconductor at high pressure
Helium (He) is the most inert noble gas at ambient conditions. It adopts a
hexagonal close packed structure (P63/mmc) and remains in the insulating phase
up to 32 TPa. In contrast, lithium (Li) is one of the most reactive metals at
zero pressure, while its cubic high-pressure phase (Fd-3m) is a weak metallic
electride above 475 GPa. Strikingly, a stable compound of Li5He2 (R-3m) was
formed by mixing Fd-3m Li with P63/mmc He above 700 GPa. The presence of helium
promotes the lattice transformation from Fd-3m Li to Pm-3m Li, and tuns the
three-dimensional distributed interstitial electrons into the mixture of zero-
and two-dimensional anionic electrons. This significantly increases the degree
of metallization at the Fermi level, consequently, the coupling of conductive
anionic electrons with the Li-dominated vibrations is the key factor to the
formation of superconducting electride Li5He2 with a transition temperature up
to 26 K, dynamically stable to pressures down to 210 GPa.Comment: 5 pages, 3 figure
Prognostic factors for severe Pneumocystis jiroveci pneumonia of non-HIV patients in intensive care unit: a bicentric retrospective study
Depletion of TRRAP induces p53-independent senescence in liver cancer by downregulating mitotic genes
Hepatocellular carcinoma (HCC) is an aggressive subtype of liver cancer with few effective treatments and the underlying mechanisms that drive HCC pathogenesis remain poorly characterized. Identifying genes and pathways essential for HCC cell growth will aid the development of new targeted therapies for HCC. Using a kinome CRISPR screen in three human HCC cell lines, we identified transformation/transcription domain-associated protein (TRRAP) as an essential gene for HCC cell proliferation. TRRAP has been implicated in oncogenic transformation, but how it functions in cancer cell proliferation is not established. Here, we show that depletion of TRRAP or its co-factor, histone acetyltransferase KAT5, inhibits HCC cell growth via induction of p53- and p21-independent senescence. Integrated cancer genomics analyses using patient data and RNA-sequencing identified mitotic genes as key TRRAP/KAT5 targets in HCC, and subsequent cell cycle analyses revealed that TRRAP- and KAT5-depleted cells are arrested at G2/M phase. Depletion of TOP2A, a mitotic gene and TRRAP/KAT5 target, was sufficient to recapitulate the senescent phenotype of TRRAP/KAT5 knockdown. CONCLUSION: Our results uncover a role for TRRAP/KAT5 in promoting HCC cell proliferation via activation of mitotic genes. Targeting the TRRAP/KAT5 complex is a potential therapeutic strategy for HCC
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CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of β-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments. Electronic supplementary material The online version of this article (doi:10.1186/s13059-017-1237-8) contains supplementary material, which is available to authorized users
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
Thickness difference induced pore structure variations in cellulosic separators for lithium-ion batteries
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