BBS consortium induced systemic tolerance to drought stress in cucumber plants.

<p>BBS strains colonized the cucumber roots, maintained a high level of root vigor to protect the root from destroy. Solid arrows indicate plant compounds affected by BBS components. The signal mobilized from the roots to the leaves, which kept SOD activity, proline content, chlorophyll content at higher levels, and kept MDA content at a lower level, to protect the integrity of plant cells, and to release oxidative stress and to remain photosynthesis, leading induced drought tolerance.</p

Effects of BBS on the expression of the <i>rbcS</i>, <i>rbcL</i>, and <i>cAPX</i> genes.

<p>The cell suspension of BBS and sterile water were poured on the soil around cucumber roots in the BBS treatment (BBS) and the control (CK), respectively, and then watering was withheld for 13 days. A, The expression of the genes <i>rbcS</i>, <i>rbcL</i> and <i>cAPX</i> was detected at 0, 5, 7, 9, 11, and 13 dpi by RT-PCR. B, Relative mRNA levels of genes based on Quality One software of Bio-Rad. Each treatment was replicated three times. dpi, day post inoculation; <i>Actin</i>, internal reference.</p

Detection of ACC deaminase activity in three BBS constituent strains and <i>Burkholderia</i> sp. 5BS21.

<p>After grown in MSA medium with ACC (ACC+) or without ACC (ACC−) for 48 h, bacterial cells of <i>Burkholderia</i> sp. 5BS21 (5BS21) and of the three BBS constituent strains <i>Bacillus cereus</i> AR156 (AR156), <i>Bacillus subtilis</i> SM21 (SM21), <i>Serratia</i> sp. XY21 (XY21) were centrifuged at 14000× g for 5 min. Then 5 µL of the supernatant resulting from each culture was spotted on a TLC silica gel plate with 5 µL of MSA medium with ACC (ACC+) and 5 µL of that without ACC (ACC−) as controls. The gel was developed for 2.5 h and then stained with 0.5% ninhydrin for 20 min in an attemperator set at 100°C.</p

Supplementary Table 3

Overview of all 15 KEGG pathway

BBS inducing drought tolerance in cucumber plants.

<p>The cell suspension of BBS and sterile water were poured on the soil around cucumber roots in the BBS treatment (BBS) and the control (CK), respectively, and then watering was withheld for 13 days.</p

Supplementary Table 4

List of primers used in real-time PCR analysis

Supplementary Table 1

Transcriptional changes that are induced following treatment with AR156 or flg22 in Arabidopsis thaliana

Supplementary Table 2

List of 1302 differentially expressed genes(DEGs) in AR156 and flg22 treatments in Arabidopsis thaliana

Additional file 1: of An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease

Supplemental Table and Figures. Supplemental Table S1 and Figures S1–S7. (PDF 742 kb