The Line-broken Analysis of the Aluminum Conductor Composite Core

Aluminum conductor composite core has many technical advantages such as high tensile strength, light weight, low sag, large current carrying capacity. In order to study the influence of the broken of aluminum conductor composite core on the transmission tower, tower-line model was built by using the finite element software ANSYS/LS-DYNA, and the deformation of joints and stress of bar were analyzed. The impact load and the impact coefficient of the representative bars of the transmission tower are calculated. The results show that the impact acting on the tower is the most serious in the place of the conductor suspension point, the connection of upper and lower crank arms, the tower neck

Optimizing the Biosynthesis of Dihydroquercetin from Naringenin in Saccharomyces cerevisiae

Dihydroquercetin (DHQ), known for its varied physiological benefits, is widely used in the food, chemical, and pharmaceutical industries. However, the efficiency of the DHQ synthesis is significantly limited by the substantial accumulation of intermediates during DHQ biosynthesis. In this study, DHQ production was achieved by integrating genes from various organisms into the yeast chromosome for the expression of flavanone-3-hydroxylase (F3H), flavonoid-3′-hydroxylase, and cytochrome P450 reductase. A computer-aided protein design approach led to the development of optimal F3H mutant P221A, resulting in a 1.67-fold increase in DHQ yield from naringenin (NAR) compared with the control. Subsequently, by analysis of the enzyme reaction and optimization of the culture medium composition, 637.29 ± 20.35 mg/L DHQ was synthesized from 800 mg/L NAR. This corresponds to a remarkable conversion rate of 71.26%, one of the highest reported values for DHQ synthesis from NAR to date

Figure S1 from <i>NRAS</i> Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth

Supplementary Figure 1. AHCYL1 is selectively critical for NRAS-mutated but not for BRAF-mutated human melanoma cells.</p

Figure S7 from <i>NRAS</i> Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth

Supplementary Figure 7. AHCYL1 deficiency attenuates cell proliferation, decreases ER calcium levels, and activates the UPR.</p

Figure S3 from <i>NRAS</i> Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth

Supplementary Figure 3. AHCYL2 does not overexpress and is not critical in human NRAS-mutated melanoma as AHCYL1.</p

Figure S11 from <i>NRAS</i> Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth

Supplementary Figure 11. There is positive correlation between ATF2 and AHCYL1 mRNA levels in human cutaneous melanoma patients by TCGA analysis.</p

Figure S10 from <i>NRAS</i> Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth

Supplementary Figure 10. CREB doesn’t positively regulate AHCYL1 transcription.</p

Figure S2 from <i>NRAS</i> Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth

Supplementary Figure 2. AHCYL1 knockout selectively causes cell cycle arrest in NRAS-mutant expressing human melanoma cells.</p

Figure S9 from <i>NRAS</i> Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth

Supplementary Figure 9. RT-qPCR validates key up/downregulated target genes from gene sets related to cell proliferation from RNA-seq.</p