Analysis of Total Flavonoids in Different Parts of Wild Planting Tetrastigma hemsleyanum Diels et Gilg and Comparison of Their Anti-inflammatory and Antioxidant Capacity

Objective: The content of total flavonoids, antioxidant activity and anti-inflammatory capacity in different parts of Tetrastigma hemsleyanum Diels et Gilg which imitated wild planting were compared. Methods: Stems, leaves, root tubers and root whiskers of wild imitating-planted Tetrastigma hemsleyanum Diels et Gilg were first extracted by alcohol extraction technology, and the content of total flavonoids was compared among different parts. Then DPPH free radical scavenging rate, ABTS+ free radical scavenging rate, hydroxyl free radical scavenging rate and ferric ion reducing power were used to evaluate the antioxidant capacity of different parts of plants. Meanwhile, lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was established to compare anti-inflammatory ability of different parts of wild planting Tetrastigma hemsleyanum Diels et Gilg by measuring the release of NO. Results: Under the optimal extraction conditions, the contents of total flavonoids in stem leaves, root tubers and root whiskers were 11.86±0.23, 8.48±0.10 and 7.52±0.02 mg·g−1, respectively. A total of 10 common peaks of Tetrastigma hemsleyanum Diels et Gilg were identified by HPLC fingerprint. Rutin (peak 6), quercetin (peak 8) and kaempferol (peak 9) were identified via comparisons with reference standards. The content of the three indexes was the highest in roots, followed by stems and leaves. The IC50 values of DPPH free radicals, ABTS+ free radicals and hydroxyl free radicals in different parts of the plant were detected, and the values of stem leaves were 0.2107, 0.2315 and 0.7625 mg/mL. Root tubers were 0.3134, 0.3758 and 0.8967 mg/mL. The roots were 0.2058, 0.2587 and 0.7856 mg/mL, respectively. The absorbance values of the reducing capacity of ferric ions in stem, tuber and root were 0.172, 0.153 and 0.184. Three parts of the wild imitating-wild plant were not toxic to RAW264.7 cells at the concentrations between 25~200 μg/mL and could effectively inhibit the release of NO induced by LPS that achieving a good anti-inflammatory effect. Conclusion: The results of this study provided a reference for the quality evaluation and a theoretical basis for waste recycling as well as subsequent whole resource development to the non-medicinal parts of Tetrastigma hemsleyanum Diels et Gilg from the wild

The First Case of Ischemia-Free Kidney Transplantation in Humans

Background: Ischemia-reperfusion injury (IRI) has been considered an inevitable event in organ transplantation since the first successful kidney transplant was performed in 1954. To avoid IRI, we have established a novel procedure called ischemia-free organ transplantation. Here, we describe the first case of ischemia-free kidney transplantation (IFKT). Materials and Methods: The kidney graft was donated by a 19-year-old brain-dead donor. The recipient was a 47-year-old man with end-stage diabetic nephropathy. The graft was procured, preserved, and implanted without cessation of blood supply using normothermic machine perfusion. Results: The graft appearance, perfusion flow, and urine production suggested that the kidney was functioning well-during the whole procedure. The creatinine dropped rapidly to normal range within 3 days post-transplantation. The levels of serum renal injury markers were low post-transplantation. No rejection or vascular or infectious complications occurred. The patient had an uneventful recovery. Conclusion: This paper marks the first case of IFKT in humans. This innovation may offer a unique solution to optimizing transplant outcomes in kidney transplantation

Research on Electromagnetic Susceptibility of Electronic Modules in Component-Level HEMP PCI Test

The study of electromagnetic sensitivity of electronic modules is crucial for the selection of a component-level pulse current injection (PCI) waveform, which will determine whether a component-level PCI test is equivalent to a system-level pulse illumination test of the system to which the electronic module belongs. For electromagnetic sensitivity analysis, the equivalence between the injection waveform and a typical high-altitude electromagnetic pulse (HEMP) conducted disturbance waveform in a component-level PCI test is studied. Based on an RF low noise amplifier (LNA) test board, component-level PCI tests were performed using 20 ns/500 ns double exponential wave and square-wave pulse with multiple pulse-widths. The damage threshold was analyzed and determined by using vector norm and its internal damage was observed and validated by optical microscopic analysis. The conclusions are demonstrated as follows: first, during square-wave PCI tests of RF LNA, the electromagnetic sensitive parameter action is divided into three regions by pulse-width range, called ∞-norm, 2-norm and competitive failure-dominating regions; second, the electromagnetic damage effect of the RF LNA is mainly caused by the burning of its two cascaded transistors, forming a pulse energy transmission channel with short-circuit impedance from the input port to the ground; third, the 100 ns-width square waveform can be determined as the equivalent injection waveform of a HEMP conducted waveform, and the pulse peak value of injected current is determined as the electromagnetic sensitive parameter for square-wave PCI tests of the RF LNA. The conclusions verified the feasibility of establishing the equivalence between different pulse waveforms according to the electromagnetic sensitivity analysis based on the vector norm theory and effect mechanism analysis

Changes in Soil Organic Carbon Fractions and Fungal Communities, Subsequent to Different Management Practices in Moso Bamboo Plantations

Moso bamboo (Phyllostachys pubescens) has an extremely fast growth rate and major carbon sequestration potential. However, little information is available on the dynamics of soil C accumulation and fungi communities related to different management practices. Here, we investigated changes in the soil organic carbon (SOC) fractions and fungal communities of a Moso bamboo plantation under three different management practices (M0: undisturbed; M1: extensively managed; and M2: intensively managed). Compared with M0, SOC levels were reduced by 41.2% and 71.5% in M1 and M2, respectively; furthermore, four SOC fractions (C1: very labile; C2: labile; C3: less labile; and C4: nonlabile) and the carbon management index (CMI) were also significantly reduced by plantation management. These practices further altered fungal communities, for example, by increasing Basidiomycota and Mortierellomycota, and by decreasing Ascomycota and Rozellomycota. Pyrenochaeta, Mortierella, Saitozyma, and Cladophialophora were identified as keystone taxa. Soil fungal communities were significantly related to the pH, NH4-N, AP, C3, and the C4 fractions of SOC. Random forest modeling identified soil C3 and Mortierella as the most important predictors of the CMI. Our results suggest that reducing human interference would be beneficial for fungal community improvement and C sequestration in Moso bamboo plantations

Research on Electromagnetic Susceptibility of Electronic Modules in Component-Level HEMP PCI Test

The study of electromagnetic sensitivity of electronic modules is crucial for the selection of a component-level pulse current injection (PCI) waveform, which will determine whether a component-level PCI test is equivalent to a system-level pulse illumination test of the system to which the electronic module belongs. For electromagnetic sensitivity analysis, the equivalence between the injection waveform and a typical high-altitude electromagnetic pulse (HEMP) conducted disturbance waveform in a component-level PCI test is studied. Based on an RF low noise amplifier (LNA) test board, component-level PCI tests were performed using 20 ns/500 ns double exponential wave and square-wave pulse with multiple pulse-widths. The damage threshold was analyzed and determined by using vector norm and its internal damage was observed and validated by optical microscopic analysis. The conclusions are demonstrated as follows: first, during square-wave PCI tests of RF LNA, the electromagnetic sensitive parameter action is divided into three regions by pulse-width range, called ∞-norm, 2-norm and competitive failure-dominating regions; second, the electromagnetic damage effect of the RF LNA is mainly caused by the burning of its two cascaded transistors, forming a pulse energy transmission channel with short-circuit impedance from the input port to the ground; third, the 100 ns-width square waveform can be determined as the equivalent injection waveform of a HEMP conducted waveform, and the pulse peak value of injected current is determined as the electromagnetic sensitive parameter for square-wave PCI tests of the RF LNA. The conclusions verified the feasibility of establishing the equivalence between different pulse waveforms according to the electromagnetic sensitivity analysis based on the vector norm theory and effect mechanism analysis

Table_1_Metagenomic insights into the characteristics of soil microbial communities in the decomposing biomass of Moso bamboo forests under different management practices.DOCX

IntroductionConsidering the rapid growth and high biomass productivity, Moso bamboo (Phyllostachys edulis) has high carbon (C) sequestration potential, and different management practices can strongly modify its C pools. Soil microorganisms play an important role in C turnover through dead plant and microbial biomass degradation. To date, little is known about how different management practices affect microbial carbohydrate-active enzymes (CAZymes) and their responses to dead biomass degradation.MethodsBased on metagenomics analysis, this study analyzed CAZymes in three comparable stands from each Moso bamboo plantation: undisturbed (M0), extensively managed (M1), and intensively managed (M2).ResultsThe results showed that the number of CAZymes encoding plant-derived component degradation was higher than that encoding microbe-derived component degradation. Compared with the M0, the CAZyme families encoding plant-derived cellulose were significantly (p DiscussionOur study established that M2 and M1 impact dead biomass decomposition and C turnover, contributing to decreased C accumulation and establishing that the bacterial community plays the main role in C turnover in bamboo plantations.</p

First definition of reference intervals of liver function tests in China: a large-population-based multi-center study about healthy adults.

BACKGROUND: Reference intervals of Liver function tests are very important for the screening, diagnosis, treatment, and monitoring of liver diseases. We aim to establish common reference intervals of liver function tests specifically for the Chinese adult population. METHODS: A total of 3210 individuals (20-79 years) were enrolled in six representative geographical regions in China. Analytes of ALT, AST, GGT, ALP, total protein, albumin and total bilirubin were measured using three analytical systems mainly used in China. The newly established reference intervals were based on the results of traceability or multiple systems, and then validated in 21 large hospitals located nationwide qualified by the National External Quality Assessment (EQA) of China. RESULTS: We had been established reference intervals of the seven liver function tests for the Chinese adult population and found there were apparent variances of reference values for the variables for partitioning analysis such as gender(ALT, GGT, total bilirubin), age(ALP, albumin) and region(total protein). More than 86% of the 21 laboratories passed the validation in all subgroup of reference intervals and overall about 95.3% to 98.8% of the 1220 validation results fell within the range of the new reference interval for all liver function tests. In comparison with the currently recommended reference intervals in China, the single side observed proportions of out of range of reference values from our study for most of the tests deviated significantly from the nominal 2.5% such as total bilirubin (15.2%), ALP (0.2%), albumin (0.0%). Most of reference intervals in our study were obviously different from that of other races. CONCLUSION: These used reference intervals are no longer applicable for the current Chinese population. We have established common reference intervals of liver function tests that are defined specifically for Chinese population and can be universally used among EQA-approved laboratories located all over China