Measuring forces between protein fibers by microscopy

We propose a general scheme for measuring the attraction between mechanically frustrated semiflexible fibers by measuring their thermal fluctuations and shape. We apply this analysis to a system of sickle hemoglobin (HbS) fibers that laterally attract one another. These fibers appear to “zip” together before reaching mechanical equilibrium due to the existence of cross-links into a dilute fiber network. We are also able to estimate the rigidities of the fibers. These rigidities are found to be consistent with sickle hemoglobin “single” fibers 20 nm in diameter, despite recent experiments indicating that fiber bundling sometimes occurs. Our estimate of the magnitude of the interfiber attraction for HbS fibers is in the range 8 ± 7 kBT/μm, or 4 ± 3 kBT/μm if the fibers are assumed, a priori to be single fibers (such an assumption is fully consistent with the data). This value is sufficient to bind the fibers, overcoming entropic effects, although extremely chemically weak. Our results are compared to models for the interfiber attraction that include depletion and van der Waals forces. This technique should also facilitate a similar analysis of other filamentous protein assembles in the future, including β-amyloid, actin, and tubulin

Development and characterisation of traceable force measurement for nanotechnology

Traceable low force metrology should be an essential tool for nanotechnology. Traceable measurement of micro- and nanonewton forces would allow independent measurement and comparison on material properties, MEMS behaviour and nanodimensional measurement uncertainties. Yet the current traceability infrastructure in the UK is incomplete. This thesis describes the incremental development of the low force facility at the National Physical Laboratory (NPL). The novel contribution of this thesis has three components. First, specific modifications to the NPL Low Force Balance were undertaken. This involved developing novel or highly modified solutions to address key issues, as well as undertaking detailed comparions with external ans internal traceability references. Second, a triskelion force sensor flexure was proposed and mathematically modelled using both analytical and finite element techniques, and compared to experimentally measured spring constant estimates. The models compared satisfactorily, though fabrication defects in developed prototype artefacts limited the experimental confirmation of the models. Third, a piezoelectric sensor approach for quasistatic force measurement was proposed, experimentally evaluated and rejected. Finally, an improved design for a low force transfer artefact system is presented, harnessing the findings of the reported investigations. The proposed design combines proven strain-sensing technology with the advantageous triskelion flexure, incorporating an external stage and packaging aspects to achieve the requirements for a traceable low force transfer artefact

Role Preference: Are Handheld Computers an Educational or Personal Technology?

As an educational delivery platform, current handheld computer technology provides a low-cost, networked, small-form factor appliance with sufficient machine resources to support instruction, learning, assessment, and collaboration. Yet, except in the fields of medicine and law, handheld adoption for collegiate classroom use has been minimal. This study presents the results of an empirical investigation of users and non-users of handheld technology in higher education. Faculty and student personal technology preferences, handheld usage practices, and experience profiles are presented. Results confirm handhelds are peripheral to most collegiate instruction with usage confined primarily to performing personal information management. When handhelds are used for education, they function as a portable extension of the personal computer. Implications for educational practice are presented

Analysis of Hydrological Drought Events in the Upper Tana Basin of Kenya

Drought is a major environmental hazard which has serious implications for water management and environmental protection. This is especially so when unsustainable water management, as well as predicted climate change effects in droughts, could result in severe impacts on nature and society. Inefficient management of drought and water resources could put aquatic ecosystems under serious severe stress. The lack of adequate water availability in rivers during drought episodes leads to heavy overexploitation of the rivers and reservoirs, which significantly affects the survival of associated biological diversity. It is therefore essential to know the occurrence of drought events in river basins with a view to establishing and developing measures to minimize the socioeconomic and environmental impacts of its effects in these areas. In this paper, drought duration and severity were examined in four homogenous regions of the upper Tana basin. The homogenous regions were established using principal component analysis results of discharge data from twenty two river gauge stations in basin. The runs analysis technique was then applied to examine the drought duration and severity at the homogenous regions of the basin. Results indicated that the mean drought duration varied from 4 to 11 months across the regions whilst the standardized mean severity ranged from 0.63 to 3.89. Two of the regions experienced nearly the same standardized mean severity. Drought events occurred at times when the basin experienced rainfall deficits an indication that low rainfall affects discharge in the rivers of the basin. The variations in the number and frequency of the drought events in the homogenous regions would possibly be related to the semi-arid and arid nature of the climate in most of the southern and eastern parts of the basin. To minimize the impacts of drought in the basin, it is recommended that capacity building of the local communities in the basin be done focusing on water conservation and alternative livelihoods systems to minimize overdependence on water resources in the basin. Keywords: Drought, hydrological drought, runs analysis, water managemen

Correlation of the Quasi-Periodic Oscillation Frequencies of White Dwarf, Neutron Star, and Black Hole Binaries

Using data obtained in 1994 June/July with the Extreme Ultraviolet Explorer deep survey photometer and in 2001 January with the Chandra X-ray Observatory Low Energy Transmission Grating Spectrograph, we investigate the extreme-ultraviolet (EUV) and soft X-ray oscillations of the dwarf nova SS Cyg in outburst. We find quasi-periodic oscillations (QPOs) at nu_0 ~ 0.012 Hz and nu_1 ~ 0.13 Hz in the EUV flux and at nu_0 ~ 0.0090 Hz, nu_1 ~ 0.11 Hz, and possibly nu_2 ~ nu_0 + nu_1 ~ 0.12 Hz in the soft X-ray flux. These data, combined with the optical data of Woudt & Warner for VW Hyi, extend the Psaltis, Belloni, & van der Klis nu_high-nu_low correlation for neutron star and black hole low-mass X-ray binaries (LMXBs) nearly two orders of magnitude in frequency, with nu_low ~ 0.08 nu_high. This correlation identifies the high-frequency quasi-coherent oscillations (so-called ``dwarf nova oscillations'') of cataclysmic variables (CVs) with the kilohertz QPOs of LMXBs, and the low-frequency QPOs of CVs with the horizontal branch oscillations (or the broad noise component identified as such) of LMXBs. Assuming that the same mechanisms produce the QPOs in white dwarf, neutron star, and black hole binaries, we find that the data exclude the relativistic precession model and the magnetospheric and sonic-point beat-frequency models (as well as any model requiring the presence or absence of a stellar surface or magnetic field); more promising are models that interpret QPOs as manifestations of disk accretion onto any low-magnetic field compact object.Comment: 15 pages including 4 encapsulated postscript figures; LaTeX format, uses aastex.cls; accepted on 2002 July 23 for publication in The Astrophysical Journa

NASA High-Reynolds Number Circulation Control Research - Overview of CFD and Planned Experiments

A new capability to test active flow control concepts and propulsion simulations at high Reynolds numbers in the National Transonic Facility at the NASA Langley Research Center is being developed. This technique is focused on the use of semi-span models due to their increased model size and relative ease of routing high-pressure air to the model. A new dual flow-path high-pressure air delivery station has been designed, along with a new high performance transonic sem -si pan wing model. The modular wind tunnel model is designed for testing circulation control concepts at both transonic cruise and low-speed high-lift conditions. The ability of the model to test other active flow control techniques will be highlighted. In addition, a new higher capacity semi-span force and moment wind tunnel balance has been completed and calibrated to enable testing at transonic conditions

System, Devices and Methods for Molecular Separation

Systems, devices and methods for molecular separation including a molecular separation device comprising at least a polycrystalline metal-organic framework (MOF) and a nanocrystalline, zeolite MFI, wherein the MOF forms a polycrystalline membrane with zeolite MFI nanoparticles dispersed therein, and the MOF membrane matrix contacting and surrounding the zeolite MFI nanoparticles form a permselective nanoporous structure

High Efficiency, High Performance Metal-Organic Framework (MOF) Membranes in Hollow Fibers and Tubular Modules

A reactor cell for measuring gas and liquid permeation is disclosed. A hollow fiber is supported by and sealed into a first hole and a second hole of the reactor module. The first and second ends of the hollow fiber are sealed with a sealing solution. Methods for making and using the reactor cell are also disclosed. As made and used, the reactor cell further comprises a molecular sieving membrane that is uniform and free of defects grown on an inner bore surface of the hollow fiber