New method for detecting & measuring cracks on concrete using fiber optic sensors

Advances in the production of optical fibers have made possible the recent development of innovative sensing systems for health monitoring of civil structures. The main reasons for this development are the reduced weight and dimensions of fiber optic sensors, the strong immunity to electromagnetic interference, the improved environmental resistance and the scale flexibility for small-gage and long-gage measurement. This paper provides an overview of the challenges in developing a new fiber optic sensor that can be employed to monitor flexural and tensile cracks on RC structures. The methodology in detecting and localizing the formation of flexural cracks in various locations and sensor’s capability in measuring a range of crack widths is demonstrated through testing of instrumented RC beams subjected to sustained and repeated loading.Fundação para a Ciência e a Tecnologia (FCT

Ebola virus VP35 interaction with dynein LC8 regulates viral RNA synthesis

Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Here we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8

Computational approach to predict species-specific type III secretion system (T3SS) effectors using single and multiple genomes

Positive (effectors), negative (non-effectors), and testing sets of the five organisms that we used in the GenSET studies. (ALL Tags denotes all proteins encoded in the genome; Positive Set denotes known effector proteins used for training; Negative Set denotes non-effector proteins used for training; Testing Set denotes all proteins in the bacteria minus the Positive and Negative Sets; Positives in Testing Set denotes known effector proteins included in the Testing Set). (XLSX 48 kb

Topoisomerase II inhibitors induce DNA damage-dependent interferon responses circumventing Ebola virus immune evasion

Ebola virus (EBOV) protein VP35 inhibits production of interferon alpha/beta (IFN) by blocking RIG-I-like receptor signaling pathways, thereby promoting virus replication and pathogenesis. A high-throughput screening assay, developed to identify compounds that either inhibit or bypass VP35 IFN-antagonist function, identified five DNA intercalators as reproducible hits from a library of bioactive compounds. Four, including doxorubicin and daunorubicin, are anthracycline antibiotics that inhibit topoisomerase II and are used clinically as chemotherapeutic drugs. These compounds were demonstrated to induce IFN responses in an ATM kinase-dependent manner and to also trigger the DNA-sensing cGAS-STING pathway of IFN induction. These compounds also suppress EBOV replication in vitro and induce IFN in the presence of IFN-antagonist proteins from multiple negative-sense RNA viruses. These findings provide new insights into signaling pathways activated by important chemotherapy drugs and identify a novel therapeutic approach for IFN induction that may be exploited to inhibit RNA virus replication

Predictions of the L[CII]_{\rm[CII]}-SFR and [CII_{\rm II}] Luminosity Function at the Epoch of Reionization

We present the first predictions for the $L_{\rm [CII]}$ - SFR relation and [CII] luminosity function (LF) in the Epoch of Reionization (EoR) based on cosmological hydrodynamics simulations using the SIMBA suite plus radiative transfer calculations via S\'IGAME. The sample consists of 11,137 galaxies covering halo mass $\log M_{\rm halo}\in$[9, 12.4] $M_\odot$, star formation rate SFR$\in$[0.01, 330] $M_\odot$ yr$^{-1}$, and metallicity $<Z_{\rm gas}>_{\rm SFR}\in$[0.1, 0.9] $Z_\odot$. The simulated $L_{\rm [CII]}$-SFR relation is consistent with the range observed, but with a spread of $\simeq$0.3 dex at the high end of SFR ($>$100 $M_\odot$ yr$^{-1}$) and $\simeq$0.6 dex at the lower end, and there is tension between our predictions and the values of $L_{\rm [CII]}$ above 10$^{8.5}$ $L_\odot$ observed in some galaxies reported in the literature. The scatter in the $L_{\rm [CII]}$-SFR relation is mostly driven by galaxy properties, such that at a given SFR, galaxies with higher molecular gas mass and metallicity have higher $L_{\rm [CII]}$. The [CII] LF predicted by SIMBA is consistent with the upper limits placed by the only existing untargeted flux-limited [CII] survey at the EoR (ASPECS) and those predicted by semi-analytic models. We compare our results with existing models and discuss differences responsible for the discrepant slopes in the $L_{\rm [CII]}$-SFR relatiion.Comment: 19 pages, 14 figures, Accepted by Ap

Brane Tilings and Exceptional Collections

Both brane tilings and exceptional collections are useful tools for describing the low energy gauge theory on a stack of D3-branes probing a Calabi-Yau singularity. We provide a dictionary that translates between these two heretofore unconnected languages. Given a brane tiling, we compute an exceptional collection of line bundles associated to the base of the non-compact Calabi-Yau threefold. Given an exceptional collection, we derive the periodic quiver of the gauge theory which is the graph theoretic dual of the brane tiling. Our results give new insight to the construction of quiver theories and their relation to geometry.Comment: 46 pages, 37 figures, JHEP3; v2: reference added, figure 13 correcte

Speed breeding for multiple quantitative traits in durum wheat

Plant breeding requires numerous generations to be cycled and evaluated before an improved cultivar is released. This lengthy process is required to introduce and test multiple traits of interest. However, a technology for rapid generation advance named 'speed breeding' was successfully deployed in bread wheat (Triticum aestivum L.) to achieve six generations per year while imposing phenotypic selection for foliar disease resistance and grain dormancy. Here, for the first time the deployment of this methodology is presented in durum wheat (Triticum durum Desf.) by integrating selection for key traits, including above and below ground traits on the same set of plants. This involved phenotyping for seminal root angle (RA), seminal root number (RN), tolerance to crown rot (CR), resistance to leaf rust (LR) and plant height (PH). In durum wheat, these traits are desirable in environments where yield is limited by in-season rainfall with the occurrence of CR and epidemics of LR. To evaluate this multi-trait screening approach, we applied selection to a large segregating F2 population (n = 1000) derived from a bi-parental cross (Outrob4/Caparoi). A weighted selection index (SI) was developed and applied. The gain for each trait was determined by evaluating F3 progeny derived from 100 'selected' and 100 'unselected' F2 individuals.Transgressive segregation was observed for all assayed traits in the Outrob4/Caparoi F2 population. Application of the SI successfully shifted the population mean for four traits, as determined by a significant mean difference between 'selected' and 'unselected' F3 families for CR tolerance, LR resistance, RA and RN. No significant shift for PH was observed.The novel multi-trait phenotyping method presents a useful tool for rapid selection of early filial generations or for the characterization of fixed lines out-of-season. Further, it offers efficient use of resources by assaying multiple traits on the same set of plants. Results suggest that when performed in parallel with speed breeding in early generations, selection will enrich recombinant inbred lines with desirable alleles and will reduce the length and number of years required to combine these traits in elite breeding populations and therefore cultivars.Samir Alahmad, Eric Dinglasan, Kung Ming Leung, Adnan Riaz, Nora Derbal, Kai P. Voss-Fels, Jason A. Able, Filippo M. Bassi, Jack Christopher and Lee T. Hicke