137 research outputs found
An integrated vertex model of the mesoderm invagination during the embryonic development of Drosophila
The mesoderm invagination of the Drosophila embryo is known as an archetypal morphogenic process. To explore the roles of the active cellular forces and the regulation of these forces, we developed an integrated vertex model that combines the regulation of morphogen expression with cell movements and tissue mechanics. Our results suggest that a successful furrow formation requires an apical tension gradient, decreased basal tension, and increased lateral tension, which corresponds to apical constriction, basal expansion, and apicobasal shortening respectively. Our model also considers the mechanical feedback which leads to an ectopic twist expression with external compression as observed in experiments. Our model predicts that ectopic invagination could happen if an external compressive gradient is applied
Avalanches and Self-Organized Criticality in Superconductors
We review the use of superconductors as a playground for the experimental
study of front roughening and avalanches. Using the magneto-optical technique,
the spatial distribution of the vortex density in the sample is monitored as a
function of time. The roughness and growth exponents corresponding to the
vortex landscape are determined and compared to the exponents that characterize
the avalanches in the framework of Self-Organized Criticality. For those
situations where a thermo-magnetic instability arises, an analytical non-linear
and non-local model is discussed, which is found to be consistent to great
detail with the experimental results. On anisotropic substrates, the anisotropy
regularizes the avalanches
Tunable high-index photonic glasses
Materials with extreme photonic properties such as maximum diffuse
reflectance, high albedo, or tunable band gaps are essential in many current
and future photonic devices and coatings. While photonic crystals, periodic
anisotropic structures, are well established, their disordered counterparts,
photonic glasses (PGs), are less understood despite their most interesting
isotropic photonic properties. Here, we introduce a controlled high index model
PG system. It is made of monodisperse spherical TiO colloids to exploit
strongly resonant Mie scattering for optimal turbidity. We report spectrally
resolved combined measurements of turbidity and light energy velocity from
large monolithic crack-free samples. This material class reveals pronounced
resonances enabled by the possibility to tune both the refractive index of the
extremely low polydisperse constituents and their radius. All our results are
rationalized by a model based on the energy coherent potential approximation,
which is free of any fitting parameter. Surprisingly good quantitative
agreement is found even at high index and elevated packing fraction. This class
of PGs may be the key to optimized tunable photonic materials and also central
to understand fundamental questions such as isotropic structural colors, random
lasing or strong light localization in 3D.Comment: Main text: 8 pages, 4 figures; Supporting Information: 5 pages, 5
figure
Observation of the critical regime near Anderson localization of light
Diffusive transport is among the most common phenomena in nature [1].
However, as predicted by Anderson [2], diffusion may break down due to
interference. This transition from diffusive transport to localization of waves
should occur for any type of classical or quantum wave in any media as long as
the wavelength becomes comparable to the transport mean free path [3].
The signatures of localization and those of absorption, or bound states, can
however be similar, such that an unequivocal proof of the existence of wave
localization in disordered bulk materials is still lacking. Here we present
measurements of time resolved non-classical diffusion of visible light in
strongly scattering samples, which cannot be explained by absorption, sample
geometry or reduction in transport velocity. Deviations from classical
diffusion increase strongly with decreasing as expected for a phase
transition. This constitutes an experimental realization of the critical regime
in the approach to Anderson localization.Comment: 5 pages, 4 figure
In vivo quantification of mechanical properties of caudal fins in adult zebrafish
Skip to Next SectionThe caudal fins of adult zebrafish are supported by multiple bony rays that are laterally interconnected by soft interray tissue. Little is known about the fin's mechanical properties that influence bending in response to hydrodynamic forces during swimming. Here, we developed an experimental setup to measure the elastic properties of caudal fins in vivo by applying micro-Newton forces to obtain bending stiffness and a tensional modulus. We detected overall bending moments of 1.5×10−9–4×10−9 N m2 along the proximal–distal axis of the appendage showing a non-monotonous pattern that was not due to the geometry of the fin itself. Surgical disruption of the interray tissues along the proximal–distal axis revealed no significant changes to the overall bending stiffness, which we confirmed by determining a tensional modulus of the interray tissue. Thus, the biophysical values suggest that the flexibility of the fin during its hydrodynamic performance predominantly relies on the mechanical properties of the rays
Hydrodynamic stress and phenotypic plasticity of the zebrafish regenerating fin
Understanding how extrinsic factors modulate genetically encoded information to produce a specific phenotype is of prime scientific interest. In particular, the feedback mechanism between abiotic forces and locomotory organs during morphogenesis to achieve efficient movement is a highly relevant example of such modulation. The study of this developmental process can provide unique insights on the transduction of cues at the interface between physics and biology. Here, we take advantage of the natural ability of adult zebrafish to regenerate their amputated fins to assess its morphogenic plasticity upon external modulations. Using a variety of surgical and chemical treatments, we could induce phenotypic responses to the structure of the fin. Through the ablation of specific rays in regenerating caudal fins, we generated artificially narrowed appendages in which the fin cleft depth and the positioning of rays bifurcations were perturbed compared with normal regenerates. To dissect the role of mechanotransduction in this process, we investigated the patterns of hydrodynamic forces acting on the surface of a zebrafish fin during regeneration by using particle tracking velocimetry on a range of biomimetic hydrofoils. This experimental approach enabled us to quantitatively compare hydrodynamic stress distributions over flapping fins of varying sizes and shapes. As a result, viscous shear stress acting on the distal margin of regenerating fins and the resulting internal tension are proposed as suitable signals for guiding the regulation of ray growth dynamics and branching pattern. Our findings suggest that mechanical forces are involved in the fine-tuning of the locomotory organ during fin morphogenesis
In-vivo imaging of the drosophila wing imaginal disc over time: Novel insights on growth and boundary formation
In developmental biology, the sequence of gene induction and pattern formation is best studied over time as an organism develops. However, in the model system of Drosophila larvae this oftentimes proves difficult due to limitations in imaging capabilities. Using the larval wing imaginal disc, we show that both overall growth, as well as the creation of patterns such as the distinction between the anterior(A) and posterior(P) compartments and the dorsal(D) and ventral(V) compartments can be studied directly by imaging the wing disc as it develops inside a larva. Imaged larvae develop normally, as can be seen by the overall growth curve of the wing disc. Yet, the fact that we can follow the development of individual discs through time provides the opportunity to simultaneously assess individual variability. We for instance find that growth rates can vary greatly over time. In addition, we observe that mechanical forces act on the wing disc within the larva at times when there is an increase in growth rates. Moreover, we observe that A/P boundary formation follows the established sequence and a smooth boundary is present from the first larval instar on. The division of the wing disc into a dorsal and a ventral compartment, on the other hand, develops quite differently. Contrary to expectation, the specification of the dorsal compartment starts with only one or two cells in the second larval instar and a smooth boundary is not formed until the third larval instar
Magnetic-field effects on one-dimensional Anderson localization of light
Transport of coherent waves in multiple-scattering media may exhibit fundamental, nonintuitive phenomena such as halt of diffusion by disorder called Anderson localization. For electromagnetic waves, this phenomenon was observed only in one and two dimensions so far. However, none of these experiments studied the contribution of reciprocal paths nor their manipulation by external fields. In order to weaken the effect of reciprocity of coherent wave transport on Anderson localization in one dimension, we studied light propagation through stacks of parallel Faraday-active glass slides exposed to magnetic fields up to 18 T. Measurements of light transmission statistics are presented and compared to one-dimensional (1D) transfer- matrix simulations. The latter reveals a self-organization of the polarization states in this system leading to a saturation of the Faraday rotation-induced reciprocity breaking, an increase of the localization length, and a decrease of transmission fluctuations when reciprocity is broken. This is confirmed experimentally for samples containing small numbers of slides while for larger samples a crossover from a 1D to a quasi-1D transport regime is found
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