An integrated vertex model of the mesoderm invagination during the embryonic development of Drosophila

The mesoderm invagination of the Drosophila embryo is known as an archetypal morphogenic process. To explore the roles of the active cellular forces and the regulation of these forces, we developed an integrated vertex model that combines the regulation of morphogen expression with cell movements and tissue mechanics. Our results suggest that a successful furrow formation requires an apical tension gradient, decreased basal tension, and increased lateral tension, which corresponds to apical constriction, basal expansion, and apicobasal shortening respectively. Our model also considers the mechanical feedback which leads to an ectopic twist expression with external compression as observed in experiments. Our model predicts that ectopic invagination could happen if an external compressive gradient is applied

Avalanches and Self-Organized Criticality in Superconductors

We review the use of superconductors as a playground for the experimental study of front roughening and avalanches. Using the magneto-optical technique, the spatial distribution of the vortex density in the sample is monitored as a function of time. The roughness and growth exponents corresponding to the vortex landscape are determined and compared to the exponents that characterize the avalanches in the framework of Self-Organized Criticality. For those situations where a thermo-magnetic instability arises, an analytical non-linear and non-local model is discussed, which is found to be consistent to great detail with the experimental results. On anisotropic substrates, the anisotropy regularizes the avalanches

Tunable high-index photonic glasses

Materials with extreme photonic properties such as maximum diffuse reflectance, high albedo, or tunable band gaps are essential in many current and future photonic devices and coatings. While photonic crystals, periodic anisotropic structures, are well established, their disordered counterparts, photonic glasses (PGs), are less understood despite their most interesting isotropic photonic properties. Here, we introduce a controlled high index model PG system. It is made of monodisperse spherical TiO$_2$ colloids to exploit strongly resonant Mie scattering for optimal turbidity. We report spectrally resolved combined measurements of turbidity and light energy velocity from large monolithic crack-free samples. This material class reveals pronounced resonances enabled by the possibility to tune both the refractive index of the extremely low polydisperse constituents and their radius. All our results are rationalized by a model based on the energy coherent potential approximation, which is free of any fitting parameter. Surprisingly good quantitative agreement is found even at high index and elevated packing fraction. This class of PGs may be the key to optimized tunable photonic materials and also central to understand fundamental questions such as isotropic structural colors, random lasing or strong light localization in 3D.Comment: Main text: 8 pages, 4 figures; Supporting Information: 5 pages, 5 figure

Observation of the critical regime near Anderson localization of light

Diffusive transport is among the most common phenomena in nature [1]. However, as predicted by Anderson [2], diffusion may break down due to interference. This transition from diffusive transport to localization of waves should occur for any type of classical or quantum wave in any media as long as the wavelength becomes comparable to the transport mean free path $\ell^*$ [3]. The signatures of localization and those of absorption, or bound states, can however be similar, such that an unequivocal proof of the existence of wave localization in disordered bulk materials is still lacking. Here we present measurements of time resolved non-classical diffusion of visible light in strongly scattering samples, which cannot be explained by absorption, sample geometry or reduction in transport velocity. Deviations from classical diffusion increase strongly with decreasing $\ell^*$ as expected for a phase transition. This constitutes an experimental realization of the critical regime in the approach to Anderson localization.Comment: 5 pages, 4 figure

In vivo quantification of mechanical properties of caudal fins in adult zebrafish

Skip to Next SectionThe caudal fins of adult zebrafish are supported by multiple bony rays that are laterally interconnected by soft interray tissue. Little is known about the fin's mechanical properties that influence bending in response to hydrodynamic forces during swimming. Here, we developed an experimental setup to measure the elastic properties of caudal fins in vivo by applying micro-Newton forces to obtain bending stiffness and a tensional modulus. We detected overall bending moments of 1.5×10−9–4×10−9 N m2 along the proximal–distal axis of the appendage showing a non-monotonous pattern that was not due to the geometry of the fin itself. Surgical disruption of the interray tissues along the proximal–distal axis revealed no significant changes to the overall bending stiffness, which we confirmed by determining a tensional modulus of the interray tissue. Thus, the biophysical values suggest that the flexibility of the fin during its hydrodynamic performance predominantly relies on the mechanical properties of the rays

Hydrodynamic stress and phenotypic plasticity of the zebrafish regenerating fin

Understanding how extrinsic factors modulate genetically encoded information to produce a specific phenotype is of prime scientific interest. In particular, the feedback mechanism between abiotic forces and locomotory organs during morphogenesis to achieve efficient movement is a highly relevant example of such modulation. The study of this developmental process can provide unique insights on the transduction of cues at the interface between physics and biology. Here, we take advantage of the natural ability of adult zebrafish to regenerate their amputated fins to assess its morphogenic plasticity upon external modulations. Using a variety of surgical and chemical treatments, we could induce phenotypic responses to the structure of the fin. Through the ablation of specific rays in regenerating caudal fins, we generated artificially narrowed appendages in which the fin cleft depth and the positioning of rays bifurcations were perturbed compared with normal regenerates. To dissect the role of mechanotransduction in this process, we investigated the patterns of hydrodynamic forces acting on the surface of a zebrafish fin during regeneration by using particle tracking velocimetry on a range of biomimetic hydrofoils. This experimental approach enabled us to quantitatively compare hydrodynamic stress distributions over flapping fins of varying sizes and shapes. As a result, viscous shear stress acting on the distal margin of regenerating fins and the resulting internal tension are proposed as suitable signals for guiding the regulation of ray growth dynamics and branching pattern. Our findings suggest that mechanical forces are involved in the fine-tuning of the locomotory organ during fin morphogenesis

In-vivo imaging of the drosophila wing imaginal disc over time: Novel insights on growth and boundary formation

In developmental biology, the sequence of gene induction and pattern formation is best studied over time as an organism develops. However, in the model system of Drosophila larvae this oftentimes proves difficult due to limitations in imaging capabilities. Using the larval wing imaginal disc, we show that both overall growth, as well as the creation of patterns such as the distinction between the anterior(A) and posterior(P) compartments and the dorsal(D) and ventral(V) compartments can be studied directly by imaging the wing disc as it develops inside a larva. Imaged larvae develop normally, as can be seen by the overall growth curve of the wing disc. Yet, the fact that we can follow the development of individual discs through time provides the opportunity to simultaneously assess individual variability. We for instance find that growth rates can vary greatly over time. In addition, we observe that mechanical forces act on the wing disc within the larva at times when there is an increase in growth rates. Moreover, we observe that A/P boundary formation follows the established sequence and a smooth boundary is present from the first larval instar on. The division of the wing disc into a dorsal and a ventral compartment, on the other hand, develops quite differently. Contrary to expectation, the specification of the dorsal compartment starts with only one or two cells in the second larval instar and a smooth boundary is not formed until the third larval instar

Magnetic-field effects on one-dimensional Anderson localization of light

Transport of coherent waves in multiple-scattering media may exhibit fundamental, nonintuitive phenomena such as halt of diffusion by disorder called Anderson localization. For electromagnetic waves, this phenomenon was observed only in one and two dimensions so far. However, none of these experiments studied the contribution of reciprocal paths nor their manipulation by external fields. In order to weaken the effect of reciprocity of coherent wave transport on Anderson localization in one dimension, we studied light propagation through stacks of parallel Faraday-active glass slides exposed to magnetic fields up to 18 T. Measurements of light transmission statistics are presented and compared to one-dimensional (1D) transfer- matrix simulations. The latter reveals a self-organization of the polarization states in this system leading to a saturation of the Faraday rotation-induced reciprocity breaking, an increase of the localization length, and a decrease of transmission fluctuations when reciprocity is broken. This is confirmed experimentally for samples containing small numbers of slides while for larger samples a crossover from a 1D to a quasi-1D transport regime is found