Characterization of the phosphotyrosyl protein phosphatase activity of calmodulin-dependent protein phosphatase.

Journal ArticleCalmodulin-dependent protein phosphatase from bovine brain and heart was assayed for phosphotyrosine and phosphoserine phosphatase activity using several substrates: 1) smooth muscle myosin light chain (LC20) phosphorylated on tyrosine or serine residues, 2) angiotensin I phosphorylated on tyrosine, and 3) synthetic phosphotyrosine- or phosphoserine-containing peptides with amino acid sequences patterned after the autophosphorylation site in Type II regulatory subunit of the cAMP-dependent protein kinase. The phosphatase was activated by Ni2+ and Mn2+, and stimulated further by calmodulin. In the presence of Ni2+ and calmodulin, it exhibited similar kinetic constants for the dephosphorylation of phosphotyrosyl LC20 (Km = 0.9 microM, and Vmax = 350 nmol/min/mg) and phosphoseryl LC20 (Km = 2.6 microM, Vmax = 690 nmol/min/mg). Dephosphorylation of phosphotyrosyl LC20 was inhibited by phosphoseryl LC20 with an apparent Ki of 2 microM. Compared to the reactions with phosphotyrosyl LC20 as the substrate, reactions with phosphotyrosine-containing oligopeptides exhibited slightly higher Km and lower Vmax values. The reaction with the phosphoseryl peptide based on the Type II regulatory subunit sequence exhibited a slightly higher Km (23 microM), but a much higher Vmax (4400 nmol/min/mg) than that with its phosphotyrosine-containing counterpart. Micromolar concentrations of Zn2+ inhibited the phosphatase activity; vanadate was less potent, and 25 mM NaF was ineffective. The study provides quantitative data to serve as a basis for comparing the ability of the calmodulin-dependent protein phosphatase to act on phosphotyrosine- and phosphoserine-containing substrates

Genetic Algorithm-Based Approaches for Solving Inexact Optimization Problems and their Applications for Municipal Solid Waste Management

This chapter proposes a genetic algorithm (GA)-based approach as an all-purpose problem-solving method for optimization problems with uncertainty. This chapter explains the GA-based method and presents details on the computation procedures involved for solving the three types of inexact optimization problems, which include the ILP, inexact quadratic programming (IQP) and inexact nonlinear programming (INLP) optimization problems

Curated Collections for Educators: Five Key Papers on Clinical Teaching

The ability to teach in the clinical setting is of paramount importance. Clinical teaching is at the heart of medical education, irrespective of the learner’s level of training. Learners desire and need effective, competent, and thoughtful clinical teaching from their instructors. However, many clinician-educators lack formal training on this important skill and thus may provide a variable experience to their learners. Although formal training of clinician-educators is standard and required in many other countries, the United States has yet to follow suit, leaving many faculty members to fend for themselves to learn these important skills. In September 2018, the Academic Life in Emergency Medicine (ALiEM) 2018-2019 Faculty Incubator program discussed the topic of clinical teaching techniques. We gathered the titles of papers that were cited, shared, and recommended within our online discussion forum and compiled the articles pertaining to the topic of clinical teaching techniques. To augment the list, the authors did a formal literature search using the search terms “teaching techniques", "clinical teaching", "medical education", "medical students", and "residents” on Google Scholar and PubMed. Finally, we posted a call for important papers on the topic of clinical teaching techniques on Twitter. Through this process, we identified 48 core articles on the topic of clinical teaching. We conducted a modified Delphi methodology to identify the key papers on the topic. In this paper, we present the five highest-rated articles based on the relevance to junior faculty and faculty developers. This article will review and summarize the articles we found to be the most impactful to improve one’s clinical teaching skills

Beyond Colonization, Commodification, and Reclamation: Recognizing and Retheorizing the Role of Religion in Hula

Given the history of colonization and commodification in Hawai`i, it is no surprise that non-traditional performances are met with critical reception. However, in this thesis, I hope to destabilize the popular binary juxtaposition of authentic Hawaiian art and (mis)appropriated tourist kitsch. I argue that hula has been Orientalized and wrongly associated with religion not only by colonizers and the tourist industry, but also by those whose response to colonization is a call for purity and authenticity in the practice of Hawaiian culture. I am specifically referring to people who romanticize and mythologize hula and Hawai`i prior to European contact. Therefore, I am interested in presenting a retheorization of hula that (1) recognizes hula as a recycled tradition, (2) acknowledges the unique history of the indigenous people of Hawai`i, (3) does not limit participation to certain bodies, and (4) acknowledges, without over-emphasizing or de-emphasizing, the role of religion in the history of hula

A Comparative Study of Human Health Impacts Due to Heavy Metal Emissions from a Conventional Lignite Coal-Fired Electricity Generation Station, with Post-Combustion, and Oxy- Fuel Combustion Capture Technologies

Carbon dioxide capture has become an important component for ensuring reduction of greenhouse gases in the atmosphere. Even though emission reduction technologies such as electrostatic precipitators (ESP) and flue gas desulfurization (FGD) are in place at most electricity-generating stations today, the large point source emitters of carbon dioxide (CO2) and other emissions, such as heavy metals, to the atmosphere are still fossil fuel electricity-generating stations. When CO2 capture is employed, these emissions can be further reduced. However, despite its important ability to reduce atmospheric emissions, the CO2 capture technology in fact still releases some emissions through its stacks into the air. Since the safety and stability of the CO2 capture technology are fundamental considerations for widespread social acceptance, the potential liability associated with the capture technology is cited as an important barrier to successful CO2 capture implementation. Liability of the technology is further clouded by a failure to clearly define what is at risk, especially regarding human health and safety. This research study will focus on investigating the risks associated with human health and safety resulting from the different versions of the technology including: (i) no capture system, (ii) post-combustion, and (iii) oxy-fuel combustion CO2 capture technology at the Boundary Dam Power Station (BDPS) in Estevan, Saskatchewan, Canada. The research objective of this study was to evaluate the risk to human health associated with the BDPS in Estevan, Saskatchewan, Canada, using the American Meteorological Society’s Environmental Protection Agency Regulatory Model (AERMOD) and cancer and non-cancer risk equations. This research presents the air dispersion modeling of the conventional lignite-fired electricity generation station at the BDPS, the inclusion of post-combustion CO2 capture technology, and the oxy-fuel carbon dioxide capture process. The heavy metals were measured near the power plant located in Estevan, Saskatchewan. This study shows that the emissions from the three stacks posed cancer risks of less than one chance in a million (1 × 10−6). There were only two emissions from the “no capture” scenario that caused inhalation cancer risks of more than 1 × 10−6. In terms of non-cancer risks, the pollutant’s concentration from the three stacks was unlikely to cause any non-cancer health effects