MOESM2 of Oestrous cycle-dependent equine uterine immune response to induced infectious endometritis

Additional file 2. Statistical results for gene expression levels of all analysed genes. ANOVA, Bonferroni and interaction model results for the pathogen recognition receptors TLR2, TLR4, NLRC5, the chemokines CCL2, CXCL9, CXCL10, CXCL11, the tissue inhibitor of metallopeptidases (TIMP1) and the antimicrobial peptides lysozyme, lipocalin (LCN2), lactoferrin, uteroferrin, secretory leukoprotease inhibitor (SLPI), uterocalin P19, secreted phospholipase A2 (sPLA2) and equine β-defensin 1 (EBD1)

Additional file 1: Figure S1. of Deep sequencing of the uterine immune response to bacteria during the equine oestrous cycle

Flow chart outlining the study design, time points of E. coli inoculations and collection of uterine samples. Three horses were assigned to group 1 and two to group 2. (PDF 12 kb

Additional file 2: Figure S2. of Deep sequencing of the uterine immune response to bacteria during the equine oestrous cycle

Heatmap of all genes analysed for significant differential expression between the two cycle stages before and 3 h after inoculation with E. coli. Each column represents one sample, while each row represents the log2 counts per million (CPM) of one gene with normalised standard scores (Z-expression values; −2/ light green to +2/ dark green). The cluster trees on the top and left margins represent the hierarchical relation between samples and gene counts, respectively. While the first letter stands for the horse, the second stands for (o)estrus (E) or dioestrus (D), 0 h stands for samples taken before inoculation and 3 h for samples taken 3 h thereafter. Note that biological replicates cluster according to their treatment with the exception of ED_3h, which clusters with the dioestrous samples before inoculation. (PNG 985 kb