Eine Kurzeinführung in das Brandenburger Mittelverteilungsmodell

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Survey of demographic challenges and discussion of human resource management strategies

Human resource management is one of the key factors in the productivity, growth and value of companies in which knowledge plays a key role. However, especially the shrinking of industrialized societies, in which the population is ageing rapidly, will dramatically influence the structure of the labor force in the next 10 to 15 years. Fortunately, decision-makers and human resource managers are increasingly responding to these challenges and drawing up strategies for managing the future of their corporations. But up to now many long-term concepts have been developed. Instead, short term activities focusing on recruiting and advertising/ self-promotion are given more attention. This is alarming. The authors of this paper compare the development of the populations in different countries and devise approaches to human resource management in times of change. Several long term strategies from different countries will be presented and the effects on headcount, productivity, qualifications, and knowledge will be discussed from a personnel economics point of view. --Demography,labor force potential,HRM,dynamic effects,strategies

Sulfite: Cytochrome \u3cem\u3ec\u3c/em\u3e Oxidoreductase from \u3cem\u3eThiobacillus novellus\u3c/em\u3e

Direct oxidation of sulfite to sulfate occurs in various photo- and chemotrophic sulfur oxidizing microorganisms as the final step in the oxidation of reduced sulfur compounds and is catalyzed by sulfite:cytochrome c oxidoreductase (EC1.8.2.1). Here we show that the enzyme from Thiobacillus novellus is a periplasmically located αβ heterodimer, consisting of a 40.6-kDa subunit containing a molybdenum cofactor and an 8.8-kDa mono-heme cytochrome c 552 subunit (midpoint redox potential, E m8.0 = +280 mV). The organic component of the molybdenum cofactor was identified as molybdopterin contained in a 1:1 ratio to the Mo content of the enzyme. Electron paramagnetic resonance spectroscopy revealed the presence of a sulfite-inducible Mo(V) signal characteristic of sulfite:acceptor oxidoreductases. However, pH-dependent changes in the electron paramagnetic resonance signal were not detected. Kinetic studies showed that the enzyme exhibits a ping-pong mechanism involving two reactive sites. K m values for sulfite and cytochrome c 550 were determined to be 27 and 4 μm, respectively; the enzyme was found to be reversibly inhibited by sulfate and various buffer ions. The sorABgenes, which encode the enzyme, appear to form an operon, which is preceded by a putative extracytoplasmic function-type promoter and contains a hairpin loop termination structure downstream ofsorB. While SorA exhibits significant similarities to known sequences of eukaryotic and bacterial sulfite:acceptor oxidoreductases, SorB does not appear to be closely related to any knownc-type cytochromes

Gefährden alternde Belegschaften die Innovationsfähigkeit deutscher Unternehmen? Ergebnisse einer ersten kritischen Analyse

Die demografische Entwicklung sowie die Heraufsetzung des Renteneintrittsalters werden in den nächsten Jahrzehnten dazu führen, dass der Anteil älterer Mitarbeiter in Unternehmen steigen wird. Gemäß der Defizithypothese bzw. des Defizitmodells wird unterstellt, dass ältere Mitarbeiter weniger innovativ seien und somit die Innovationsfähigkeit von Unternehmen durch das Altern der Belegschaft gefährdet würde. Für den vorliegenden Artikel wurde mit Hilfe des Mannheimer Innovationspanels aus dem Jahr 2001 eine Sekundäranalyse durchgeführt. Die Ergebnisse konnten jedoch keinen Einfluss des Anteils älterer Mitarbeiter in Unternehmen auf den Innovationsoutput nachweisen. Auch auf die Aufwendungen für Weiterbildung im Zusammenhang mit Innovationsprojekten wirkt sich der Anteil älterer Mitarbeiter im Unternehmen nicht aus. Dagegen bestätigte sich, dass bei einem hohen Anteil älterer Mitarbeiter im Unternehmen weniger in die Weiterbildung der Mitarbeiter insgesamt investiert wird. Hierfür gibt es zwei Erklärungsansätze: Entweder sind ältere Mitarbeiter kaum an Innovationsprojekten beteiligt und / oder es sind hauptsächlich die hoch qualifizierten älteren Mitarbeiter, die durch das Unternehmen gehalten werden und weiterhin in Innovationsaktivitäten integriert sind. Gefordert werden neue Instrumente zur Identifikation innovativer Mitarbeitertypen, um entsprechende Innovationsteams zusammen setzen zu können sowie eine angepasste und innovationsorientierte Personalentwicklung zu ermöglichen. --

Zebrafish eda and edar Mutants Reveal Conserved and Ancestral Roles of Ectodysplasin Signaling in Vertebrates

The genetic basis of the development and variation of adult form of vertebrates is not well understood. To address this problem, we performed a mutant screen to identify genes essential for the formation of adult skeletal structures of the zebrafish. Here, we describe the phenotypic and molecular characterization of a set of mutants showing loss of adult structures of the dermal skeleton, such as the rays of the fins and the scales, as well as the pharyngeal teeth. The mutations represent adult-viable, loss of function alleles in the ectodysplasin (eda) and ectodysplasin receptor (edar) genes. These genes are frequently mutated in the human hereditary disease hypohidrotic ectodermal dysplasia (HED; OMIM 224900, 305100) that affects the development of integumentary appendages such as hair and teeth. We find mutations in zebrafish edar that affect similar residues as mutated in human cases of HED and show similar phenotypic consequences. eda and edar are not required for early zebrafish development, but are rather specific for the development of adult skeletal and dental structures. We find that the defects of the fins and scales are due to the role of Eda signaling in organizing epidermal cells into discrete signaling centers of the scale epidermal placode and fin fold. Our genetic analysis demonstrates dose-sensitive and organ-specific response to alteration in levels of Eda signaling. In addition, we show substantial buffering of the effect of loss of edar function in different genetic backgrounds, suggesting canalization of this developmental system. We uncover a previously unknown role of Eda signaling in teleosts and show conservation of the developmental mechanisms involved in the formation and variation of both integumentary appendages and limbs. Lastly, our findings point to the utility of adult genetic screens in the zebrafish in identifying essential developmental processes involved in human disease and in morphological evolution

17-AAG Induces Cytoplasmic α-Synuclein Aggregate Clearance by Induction of Autophagy

The accumulation and aggregation of α-synuclein in nerve cells and glia are characteristic features of a number of neurodegenerative diseases termed synucleinopathies. α-Synuclein is a highly soluble protein which in a nucleation dependent process is capable of self-aggregation. The causes underlying aggregate formation are not yet understood, impairment of the proteolytic degradation systems might be involved.Cl the aggregate clearing effects of 17-AAG were abolished and α-synuclein deposits were enlarged. Analysis of LC3-II immunoreactivity, which is an indicator of autophagosome formation, further revealed that 17-AAG led to the recruitment of LC3-II and to the formation of LC3 positive puncta. This effect was also observed in cultured oligodendrocytes derived from the brains of newborn rats. Inhibition of macroautophagy by 3-methyladenine prevented 17-AAG induced occurrence of LC3 positive puncta as well as the removal of α-synuclein aggregates in OLN-A53T cells.Our data demonstrate for the first time that 17-AAG not only causes the upregulation of heat shock proteins, but also is an effective inducer of the autophagic pathway by which α-synuclein can be removed. Hence geldanamycin derivatives may provide a means to modulate autophagy in neural cells, thereby ameliorating pathogenic aggregate formation and protecting the cells during disease and aging

Antimicrobial and Biocide Resistance among Feline and Canine Staphylococcus aureus and Staphylococcus pseudintermedius Isolates from Diagnostic Submissions

A total of 114 Staphylococcus isolates from various infections of companion animals, including 43 feline Staphylococcus aureus, 19 canine S. aureus, 11 feline Staphylococcus pseudintermedius and 41 canine S. pseudintermedius were investigated for (i) their susceptibility to 24 antimicrobial agents and three combinations of antimicrobial agents by broth microdilution following CLSI recommendations and (ii) the corresponding resistance genes. In addition, the isolates were tested for their susceptibility to the four biocides benzalkonium chloride, chlorhexidine, polyhexanide and octenidine by a recently developed biocide susceptibility testing protocol. Penicillin resistance via blaZ was the dominant resistance property in all four groups of isolates ranging between 76.7 and 90.9%. About one quarter of the isolates (25.4%) proved to be methicillin-resistant and carried the genes mecA or mecC. Macrolide resistance was the second most prevalent resistance property (27.2%) and all isolates harbored the resistance genes erm(A), erm(B), erm(C), erm(T) or msr(A), alone or in combinations. Fluoroquinolone resistance was detected in 21.1% of all isolates tested, whereas tetracycline resistance via tet(K) and/or tet(M) occurred in 19.3% of the isolates. Resistance to last resort antimicrobial agents in human medicine was seen only in single isolates, if at all. The minimal inhibitory concentrations (MICs) of the four biocides showed unimodal distributions and were very similar for the four groups of staphylococci. Because of the large number of (multi)resistant isolates, antimicrobial susceptibility testing of feline and canine S. aureus and S. pseudintermedius isolates is highly recommended before the start of an antimicrobial chemotherapy. Moreover, no hints towards the development of biocide resistance were detected

Antimicrobial and Biocide Resistance among Canine and Feline Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii Isolates from Diagnostic Submissions

A total of 215 isolates from infections of dogs and cats, including 49 Enterococcus faecalis, 37 Enterococcus faecium, 59 Escherichia coli, 56 Pseudomonas aeruginosa, and 14 Acinetobacter baumannii, were investigated for their susceptibility to 27 (Gram-positive bacteria) or 20 (Gram-negative bacteria) antimicrobial agents/combinations of antimicrobial agents by broth microdilution according to the recommendations of the Clinical and Laboratory Standards Institute. Moreover, all isolates were analysed for their susceptibility to the biocides benzalkonium chloride, chlorhexidine, polyhexanide, and octenidine by a recently published broth microdilution biocide susceptibility testing method. While the E. faecalis isolates did not show expanded resistances, considerable numbers of the E. faecium isolates were resistant to penicillins, macrolides, tetracyclines, and fluoroquinolones. Even a single vancomycin-resistant isolate that carried the vanA gene cluster was detected. Expanded multiresistance phenotypes were also detected among the E. coli isolates, including a single carbapenem-resistant, blaOXA-48-positive isolate. In addition, multiresistant A. baumannii isolates were detected. The minimal inhibitory concentrations of the biocides showed unimodal distributions but differed with respect to the biocide and the bacterial species investigated. Although there were no indications of a development of biocide resistance, some P. aeruginosa isolates exhibited benzalkonium MICs higher than the highest test concentration