6 research outputs found
Surface Modification of Polymeric Biomaterials Using Recombinant Spider Silk Proteins
The performance of
biomaterials largely depends on the materials
biocompatibility, which is directly related to unwanted side effects
like foreign body responses and inflammation, and the potential of
interaction of cells with its surface, for example, cell adhesion.
In the distinct application of catheters, low or even no cell adhesion
is eligible. To influence the properties of existing and commonly
used biomaterials and to further increase their biocompatibility,
a coating with a recombinantly produced spider silk protein as outer
layer was applied on three selected catheter polymers (polyurethane,
polytetrafluoroethylene, silicone) and evaluated based on cell adhesion.
The tested cell types, HaCaT keratinocytes (epidermal cells), B50
neuronal cells, C2C12 myoblasts (muscle cells) and BALB/3T3 fibroblasts
(connective tissue), exhibited low or no adhesion on the silk-coated
materials. In combination with the lack of toxicity, the good biocompatibility,
and the low body response, it could be shown that silk coatings have
a high potential as a biomedical coating material, e.g., for catheters
<i>In Vivo</i> Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides
Magnetosomes are
natural magnetic nanoparticles with exceptional
properties that are synthesized in magnetotactic bacteria by a highly
regulated biomineralization process. Their usability in many applications
could be further improved by encapsulation in biocompatible polymers.
In this study, we explored the production of spider silk-inspired
peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion
of different silk sequence-like variants to abundant magnetosome membrane
proteins enhanced magnetite biomineralization and caused the formation
of a proteinaceous capsule, which increased the colloidal stability
of isolated particles. Furthermore, we show that spider silk peptides
fused to a magnetosome membrane protein can be used as seeds for silk
fibril growth on the magnetosome surface. In summary, we demonstrate
that the combination of two different biogenic materials generates
a genetically encoded hybrid composite with engineerable new properties
and enhanced potential for various applications
<i>In Vivo</i> Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides
Magnetosomes are
natural magnetic nanoparticles with exceptional
properties that are synthesized in magnetotactic bacteria by a highly
regulated biomineralization process. Their usability in many applications
could be further improved by encapsulation in biocompatible polymers.
In this study, we explored the production of spider silk-inspired
peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion
of different silk sequence-like variants to abundant magnetosome membrane
proteins enhanced magnetite biomineralization and caused the formation
of a proteinaceous capsule, which increased the colloidal stability
of isolated particles. Furthermore, we show that spider silk peptides
fused to a magnetosome membrane protein can be used as seeds for silk
fibril growth on the magnetosome surface. In summary, we demonstrate
that the combination of two different biogenic materials generates
a genetically encoded hybrid composite with engineerable new properties
and enhanced potential for various applications