Results of mismatch distribution analyses for Lake Donggi Cona <i>Radix</i> specimens based on mtDNA.

<p>SSD = sum of squared deviations from the respective model; RI = raggedness index; τ = population parameter tau with 95% confidence interval; <i>P</i> = significance of the respective parameter (level 0.05).</p

Sampling sites and phylogenetic relationships of <i>Radix</i> specimens sampled in the Lake Donggi Cona drainage system.

<p>(A) Lake Donggi Cona drainage system showing the lake, the perennial eastern and northeastern inflows (thick lines), some seasonal inflows, and the gauge-controlled outflow. Lake map redrawn from Dietze et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0160286#pone.0160286.ref021" target="_blank">21</a>]. Satellite image (upper right corner) taken from Natural Earth (free vector and raster map data at naturalearthdata.com). (B) Collapsed Bayesian-inference tree based on mtDNA (COI) data. The uncollapsed tree is given in the Supplement (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0160286#pone.0160286.s001" target="_blank">S1 Fig</a>).</p

NeighborNet of Lake Donggi Cona <i>Radix</i> specimens based on AFLP data.

<p>Lake specimens are colored in red; drainage specimens in orange. For specimen and locality details see the Supplement (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0160286#pone.0160286.s002" target="_blank">S1 Table</a>).</p

STRUCTURE output (K = 8) for Lake Donggi Cona <i>Radix</i> specimens based on AFLP data.

<p>Each group assignment is represented by a colored horizontal bar. (A) Accessions ordered according to their cluster membership (K = 8: A–H). (B) Accessions ordered according to their sampling site (lake: DC04, 05, 11; drainage system: DC07, 08, 10, 12, 18, 21). (C) Geographical presentation of the structure results. Red circles: sampling sites within the lake, orange circles: sampling sites within the drainage system. Lake map redrawn from Dietze et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0160286#pone.0160286.ref021" target="_blank">21</a>].</p

BEAST nex files

The ZIP folder contains all nex files used for the single gene BEAST analyses according to dataset numbers in the publicatio

long_realign

The DNA data file of the four partial loci sequenced during the present stud

Table S1

Testing between different models for the divergence time estimation using external rates and one fossil point

Table S3

Results of the test correlating the biogeographic areas

Table S2

Testing of different biogeographic scenarios using BioGeoBears

Supplementary material

DNA substitution models for each of the four loc