Decomposer biomass in the rhizosphere to assess rhizodposition

Quantification of the organic carbon released from plant roots is a challenge. These compounds of rhizodeposition are quickly transformed into CO2 and eventually bacterial biomass to be consumed by bacterivores (protozoa and nematodes). Microbes stimulate rhizodeposition several-fold so assays under sterile conditions give an unrealistic value. Quantifying bacterial production from H-3-thymidine incorporation falls short in the rhizosphere and the use of isotopes does not allow clear distinction between labeled CO2 released from roots or microbes. We reduced rhizodeposition in 3-5 week old barley with a 2 week leaf aphid attack and found that biomass of bacterivores but not bacteria in the rhizosphere correlated with plant-induced respiration activity belowground. This indicated top-down control of the bacteria. Moreover, at increasing density of aphids, bacterivore biomass in the rhizosphere decreased to the level in soil unaffected by roots. This suggests that difference in bacterivore biomass directly reflects variations in rhizodeposition. Rhizodeposition is estimated from plant-induced increases in bacterial and bacterivore biomass, and yield factors, maintenance requirements, and turnover rates from the literature. We use literature values that maximize requirements for organic carbon and still estimate the total organic rhizodeposition to be as little as 4-6% of the plant-induced respiration belowground

Nematode migration and nutrient diffusion between vetch and barley material in soil

This paper deals with migration of nematodes along nutrient gradients in soil. Portions of barley straw and green vetch leaves were mixed with soil and buried at 6, 12, 18, and 50mm distance from each other in soil. During the following 12 weeks respiration activity, microbial (SIR) biomass, nitrogen limitation of respiration activity in soil slurries all indicated that nitrogen was transferred in the soil from the nutrient rich vetch to the nutrient poor barley at least during the first 3 weeks of the experiment. Twelve out of 39 taxonomic groups of nematodes showed different growth in the two plant material-soil mixtures. Only one of these taxonomic groups (long rhabditid larvae) suggested that migration could have contributed to population development; for three other groups (short rhabditid larvae, Aphelenchoides, and Bursilla) nutrient transport through the soil was the likely mechanism for a distance-dependant population development. We suggest that for most microbivorous nematodes, except larvae of fast growing bacterivores, migration over distances exceeding one centimetre does not contribute markedly to population development even when cues such as nutrient gradients to stimulate the activity exis

Style shifts in Japanese video game commentary monologues

Honorific markers play an integral role in many languages, but their purpose and meaning are still widely debated. Ide (1989) claims a one-to-one relationship between social rank difference and linguistic form, but Cook (1997, 2011) proposes that honorifics are used to display a ‘disciplined self,’ which further indexes a variety of social meanings. This study examined style shifts between the honorific and plain form in Japanese video game commentary monologues. We found that instances of honorific form can be grouped into four categories regarding their contextual functions: to make an announcement, to indicate seriousness, conventionalized formulae, and to quote others. This conclusion supports Cook’s proposal

Is Embedding Entailed in Consumer Valuation of Food Safety Characteristics?

Consumers’ preferences for food safety characteristics are investigated with particular focus on existence of an embedding effect. Embedding exists if consumer valuation of food safety is insensitive to scope. Two choice experiments have been conducted valuing food safety in respectively minced pork and chicken breasts, exemplified by avoiding human risks of Salmonella infections and strengthening the restrictions of using antibiotics in the pork production and in terms of avoiding human risks of Salmonella and Campylobacter infections respectively. The results showed no indications of an embedding effect between the food safety characteristics, in neither of the cases.Valuation, Choice Experiment, Market Goods, Food Safety, Embedding., Agricultural and Food Policy, Food Consumption/Nutrition/Food Safety,

Cerebral perfusion measurement in brain death with intravoxel incoherent motion imaging

The assessment of brain death can be challenging in critically ill patients, and cerebral perfusion quantification might give information on the brain tissue viability. Intravoxel incoherent motion perfusion imaging is a magnetic resonance imaging technique, which extracts perfusion information from a diffusion-weighted sequence, and provides local, microvascular perfusion assessment without contrast media injection. Diffusion weighted images were acquired with 16 b-values (0–900 s/mm2) in the brain in two patients with cerebral death, confirmed by clinical assessment and evolution, as well as in two age-matched healthy subjects. The intravoxel incoherent motion perfusion fraction maps were obtained by fitting the bi-exponential signal equation model. 8 regions of interest were drawn blindly in the brain neocortex (in the frontal, temporal, parietal, and occipital lobes on both sides) and perfusion fractions were compared between patients with cerebral death and healthy control. Statistical significance was assessed using two-sided Wilcoxon signed rank test, and set to α < 0.05. Intravoxel incoherent motion (IVIM) perfusion fraction was vanishing in the brain of the two patients with cerebral brain death compared to the healthy controls. Mean (± standard deviation) cortex perfusion fraction was 0.016 ± 0.005 respectively 0.005 ± 0.008 in the cerebral death patients, compared to respectively 0.052 ± 0.021 (p = 0.02) and 0.071 ± 0.042 (p = 0.008) in the age-matched controls. Intravoxel incoherent motion perfusion imaging is a promising tool to assess local brain tissue viability in critically ill patients

Evaluation Distance Measures Between Gaussian Mixture Models of MFCCs

In music similarity and in the related task of genre classification, a distance measure between Gaussian mixture models is frequently needed. We present a comparison of the Kullback-Leibler distance, the earth movers distance and the normalized L2 distance for this application. Although the normalized L2 distance was slightly inferior to the Kullback-Leibler distance with respect to classification performance, it has the advantage of obeying the triangle inequality, which allows for efficient searching

Enhanced priming of old, not new soil carbon at elevated atmospheric CO2

Rising atmospheric CO2 concentrations accompanied by global warming and altered precipitation patterns calls for assessment of long-term effects of these global changes on carbon (C) dynamics in terrestrial ecosystems, as changes in net C exchange between soil and atmosphere will impact the atmospheric CO2 concentration profoundly. In many ecosystems, including the heath/grassland system studied here, increased plant production at elevated CO2 increase fresh C input from litter and root exudates to the soil and concurrently decrease soil N availability. Supply of labile C to the soil may accelerate the decomposition of soil organic C (SOC), a phenomenon termed ‘the priming effect’, and the priming effect is most pronounced at low soil N availability. Hence, we hypothesized that priming of SOC decomposition in response to labile C addition would increase in soil exposed to long-term elevated CO2 exposure. Further, we hypothesized that long-term warming would enhance SOC priming rates, whereas drought would decrease the priming response. We incubated soil from a long-term, full-factorial climate change field experiment, with the factors elevated atmospheric CO2 concentration, warming and prolonged summer drought with either labile C (sucrose) or water to assess the impact of labile C on SOC dynamics. We used sucrose with a 13C/12C signature that is distinct from that of the native SOC, which allowed us to assess the contribution of these two C sources to the CO2 evolved. Sucrose induced priming of SOC, and the priming response was higher in soil exposed to long-term elevated CO2 treatment. Drought tended to decrease the priming response, whereas long-term warming did not affect the level of priming significantly. We were also able to assess whether SOC-derived primed C in elevated CO2 soil was assimilated before or after the initiation of the CO2 treatment 8 years prior to sampling, because CO2 concentrations were raised by fumigating the experimental plots with pure CO2 that was 13C-depleted compared to ambient CO2. Surprisingly, we conclude that sucrose addition primed decomposition of relatively old SOC fractions, i.e. SOC assimilated more than 8 years before sampling