3 research outputs found
Sampling of Wheat Dust and Subsequent Analysis of Deoxynivalenol by LC-MS/MS
An LC-MS/MS method was developed
and validated for the determination
of deoxynivalenol in wheat dust. Extraction was carried out with acetonitrile/water/acetic
acid (79/20/1, v/v/v) followed by a hexane defatting step. Analysis
was performed using a Waters Acquity UPLC system coupled to a Quattro
Premier XE mass spectrometer. The method was validated according to
the criteria mentioned in Commission Decision 2002/657/EC. Due to
a high contamination level of wheat dust compared to wheat, limit
of detection and limit of quantitation levels of 358 ng/g and 717
ng/g, respectively, were obtained. A small survey was executed on
raw wheat materials and their corresponding dust samples (<i>n</i> = 12). The samples were analyzed according to the developed
procedure. A linear correlation (<i>R</i><sup>2</sup> =
0.941) was found for the deoxynivalenol concentration in dust versus
the deoxynivalenol concentration in wheat. Therefore, it would be
possible to estimate the cereal contamination through dust contamination