A Model for Sustainable Courier Services: Vehicle Routing with Exclusive Lanes

In Southeast Asian cities, it is common for logistic companies to operate a heterogeneous fleet of delivery vehicles with motorcycles being the preferred vehicle to handle the final phase of delivery. In such scenarios, heterogeneous fleet vehicle routing problem (HFVRP) is generally applied to plan an optimal delivery. However, in many downtown cores of large and rapidly developing Southeast Asian cities, HFVRP is neither viable nor reliable because of road usage restrictions. The purpose of this article is to develop and test a different approach that accurately takes these restrictions into account and provides viable and more sustainable results. Restrictions in this paper refer to situations of urban areas in Vietnam where (i) certain vehicle types are prohibited in specified areas or where narrow alleyways limit the utilization of vehicles that exceed the road capacity and (ii) certain roads are exclusive to certain vehicle types. In networks, limited access and exclusive lanes are represented as links, or arcs, exclusive to one or another. Taking these limitations into consideration, we have developed a unique model, which we have termed Vehicle Routing Problem with Exclusive Links (VRP-EL). The model was validated and tested for its performance on scenarios with varying ratios of exclusive links. Scenarios up to 500 customers were tested on a meta-heuristic algorithm, simulated annealing. VRP-EL produces realistic outcomes. Limiting certain links to be selected according to vehicle types increases overall travel distance. However, this increase outweighs the cost of re-planning and rerouting had they not been constrained initially. The reduction in traveling distance leads to fossil fuel reduction for the overall system. The estimation of reduced carbon emissions through applying the proposed model is presented. Considering the severe traffic congestion and carbon emissions caused by motorcycles in Vietnam, the proposed model leads to a sustainable road environment. Document type: Articl

CD4+ T cells from MHC II-dependent thymocyte–thymocyte interaction provide efficient help for B cells

Recently, a novel CD4+ T-cell developmental pathway was reported that generates thymocyte–thymocyte (T–T) CD4+ T cells. We established a mouse system (CIITAtgCIITApIV−/−) where thymic positive selection occurred only by major histocompatibility complex (MHC) class II+ thymocytes. T–T CD4+ T cells selected via MHC class II-dependent T–T interaction are comprised of PLZF-negative and innate PLZF-positive populations. Until recently, the functional role of the PLZF-negative population was unclear. In this study, we demonstrate that naïve T–T CD4+ T cells provide B-cell help to a level comparable with that of naïve conventional CD4+ T cells. Considering the absence of PLZF expression in naïve T–T CD4+ T cells, these results suggest that PLZF-negative naïve T–T CD4+ T cells are functionally equivalent to conventional naïve CD4+ T cells in terms of B-cell help

Gravitational-wave Electromagnetic Counterpart Korean Observatory (GECKO): GECKO Follow-up Observation of GW190425

One of the keys to the success of multimessenger astronomy is the rapid identification of the electromagnetic wave counterpart, kilonova (KN), of the gravitational-wave (GW) event. Despite its importance, it is hard to find a KN associated with a GW event, due to a poorly constrained GW localization map and numerous signals that could be confused as a KN. Here, we present the Gravitational-wave Electromagnetic wave Counterpart Korean Observatory (GECKO) project, the GECKO observation of GW190425, and prospects of GECKO in the fourth observing run (O4) of the GW detectors. We outline our follow-up observation strategies during O3. In particular, we describe our galaxy-targeted observation criteria that prioritize based on galaxy properties. Armed with this strategy, we performed an optical and/or near-infrared follow-up observation of GW190425, the first binary neutron star merger event during the O3 run. Despite a vast localization area of 7460 deg^2, we observed 621 host galaxy candidates, corresponding to 29.5% of the scores we assigned, with most of them observed within the first 3 days of the GW event. Ten transients were discovered during this search, including a new transient with a host galaxy. No plausible KN was found, but we were still able to constrain the properties of potential KNe using upper limits. The GECKO observation demonstrates that GECKO can possibly uncover a GW170817-like KN at a distance less than 200 Mpc if the localization area is of the order of hundreds of square degrees, providing a bright prospect for the identification of GW electromagnetic wave counterparts during the O4 run.Comment: 35 pages, 19 figures Accepted for publication in Ap

Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing

The N-terminal tail of histone H2B is believed to be involved in gene silencing, but how it exerts its function remains elusive. Here, we report the biochemical characterization of p14ARF tumor suppressor as a transcriptional repressor that selectively recognizes the unacetylated H2B tails on nucleosomes. The p14ARF–H2B tail interaction is functional, as the antagonistic effect of p14ARF on chromatin transcription is lost upon deletion or acetylation of H2B tails. Gene expression profiling and chromatin immunoprecipitation studies emphasize the significance of H2B deacetylation and p14ARF recruitment in establishing a repressive environment over the cell cycle regulatory genes. Moreover, HDAC1-mediated H2B deacetylation, especially at K20, constitutes an essential step in tethering p14ARF near target promoters. Our results thus reveal a hitherto unknown role of p14ARF in the regulation of chromatin transcription, as well as molecular mechanisms governing the repressive action of p14ARF

Highly sensitive near-infrared SERS nanoprobes for in vivo imaging using gold-assembled silica nanoparticles with controllable nanogaps

Abstract Background To take advantages, such as multiplex capacity, non-photobleaching property, and high sensitivity, of surface-enhanced Raman scattering (SERS)-based in vivo imaging, development of highly enhanced SERS nanoprobes in near-infrared (NIR) region is needed. A well-controlled morphology and biocompatibility are essential features of NIR SERS nanoprobes. Gold (Au)-assembled nanostructures with controllable nanogaps with highly enhanced SERS signals within multiple hotspots could be a breakthrough. Results Au-assembled silica (SiO2) nanoparticles (NPs) (SiO2@Au@Au NPs) as NIR SERS nanoprobes are synthesized using the seed-mediated growth method. SiO2@Au@Au NPs using six different sizes of Au NPs (SiO2@Au@Au50–SiO2@Au@Au500) were prepared by controlling the concentration of Au precursor in the growth step. The nanogaps between Au NPs on the SiO2 surface could be controlled from 4.16 to 0.98nm by adjusting the concentration of Au precursor (hence increasing Au NP sizes), which resulted in the formation of effective SERS hotspots. SiO2@Au@Au500 NPs with a 0.98-nm gap showed a high SERS enhancement factor of approximately 3.8 × 106 under 785-nm photoexcitation. SiO2@Au@Au500 nanoprobes showed detectable in vivo SERS signals at a concentration of 16μg/mL in animal tissue specimen at a depth of 7mm. SiO2@Au@Au500 NPs with 14 different Raman label compounds exhibited distinct SERS signals upon subcutaneous injection into nude mice. Conclusions SiO2@Au@Au NPs showed high potential for in vivo applications as multiplex nanoprobes with high SERS sensitivity in the NIR region. Graphical Abstrac

Dynamic Capability of the Firm as Driver of Green Supply Chain Management Implementation

Eco friendly management has received attention from business as an important competitive source. Thus, an emerging body of studies is interested in the implementation of green supply chain management (GSCM). Previous research in this field has acknowledged the importance of GSCM. However, the capability that enables a firm to successfully adopt GSCM is not well researched. This study empirically examines dynamic capability as an antecedent of GSCM, with the aim of proposing a causal relationship between dynamic capability and GSCM. The result of this study shows that implementing GSCM with dynamic capability enables a firm to achieve successful GSCM practices, and that this in turn leads to enhanced organizational performance

Exploring Technological Trends in Logistics: Topic Modeling-Based Patent Analysis

With the strategic importance of discerning opportunities and threats from technological development to achieve sustainable competitiveness, exploring technological trends becomes critical for a successful technology strategy in logistics. Given the rapid pace of development and varying technological options, logistics also increasingly requires methodological support and appropriate data to reduce the complexity and burden of exploring technology trends. While previous research has largely relied on experts’ insights, the value of patent-based approaches for exploring technological trends has been underestimated in logistics. To address this gap, this study proposes a topic modeling-based approach using logistics-related patents registered at the United States Patents and Trademark Office (USPTO). The core of the suggested approach is latent Dirichlet allocation (LDA), allowing the identification of logistics-related technological topics behind patents. The topics identified by LDA are further investigated regarding both filed-level and firm-level trends. The suggested approach is expected to offer implications of the use of patents for the purpose of exploring the trends of technology development outside the organization in logistics. In addition, we believe that the information on the technological topics and their trends generated by the suggested approach can offer an enhanced understanding of the technological landscape in logistics

A Model for Sustainable Courier Services: Vehicle Routing with Exclusive Lanes

In Southeast Asian cities, it is common for logistic companies to operate a heterogeneous fleet of delivery vehicles with motorcycles being the preferred vehicle to handle the final phase of delivery. In such scenarios, heterogeneous fleet vehicle routing problem (HFVRP) is generally applied to plan an optimal delivery. However, in many downtown cores of large and rapidly developing Southeast Asian cities, HFVRP is neither viable nor reliable because of road usage restrictions. The purpose of this article is to develop and test a different approach that accurately takes these restrictions into account and provides viable and more sustainable results. Restrictions in this paper refer to situations of urban areas in Vietnam where (i) certain vehicle types are prohibited in specified areas or where narrow alleyways limit the utilization of vehicles that exceed the road capacity and (ii) certain roads are exclusive to certain vehicle types. In networks, limited access and exclusive lanes are represented as links, or arcs, exclusive to one or another. Taking these limitations into consideration, we have developed a unique model, which we have termed Vehicle Routing Problem with Exclusive Links (VRP-EL). The model was validated and tested for its performance on scenarios with varying ratios of exclusive links. Scenarios up to 500 customers were tested on a meta-heuristic algorithm, simulated annealing. VRP-EL produces realistic outcomes. Limiting certain links to be selected according to vehicle types increases overall travel distance. However, this increase outweighs the cost of re-planning and rerouting had they not been constrained initially. The reduction in traveling distance leads to fossil fuel reduction for the overall system. The estimation of reduced carbon emissions through applying the proposed model is presented. Considering the severe traffic congestion and carbon emissions caused by motorcycles in Vietnam, the proposed model leads to a sustainable road environment