Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.

Alpha1,2-fucosyltransferases, FUT1 and FUT2, which transfer fucoses onto the terminal galactose of N-acetyl-lactosamine via α1,2-linkage have been shown to be highly expressed in various types of cancers. A few studies have shown the involvement of FUT1 substrates in tumor cell proliferation and migration. Lysosome-associated membrane protein 1, LAMP-1, has been reported to carry alpha1,2-fucosylated Lewis Y (LeY) antigens in breast cancer cells, however, the biological functions of LeY on LAMP-1 remain largely unknown. Whether or not its family member, LAMP-2, displays similar modifications and functions as LAMP-1 has not yet been addressed. In this study, we have presented evidence supporting that both LAMP-1 and 2 are substrates for FUT1, but not FUT2. We have also demonstrated the presence of H2 and LeY antigens on LAMP-1 by a targeted nanoLC-MS(3) and the decreased levels of fucosylation on LAMP-2 by MALDI-TOF analysis upon FUT1 knockdown. In addition, we found that the expression of LeY was substantial in less invasive ER+/PR+/HER- breast cancer cells (MCF-7 and T47D) but negligible in highly invasive triple-negative MDA-MB-231 cells, of which LeY levels were correlated with the levels of LeY carried by LAMP-1 and 2. Intriguingly, we also observed a striking change in the subcellular localization of lysosomes upon FUT1 knockdown from peripheral distribution of LAMP-1 and 2 to a preferential perinuclear accumulation. Besides that, knockdown of FUT1 led to an increased rate of autophagic flux along with diminished activity of mammalian target of rapamycin complex 1 (mTORC1) and enhanced autophagosome-lysosome fusion. This may be associated with the predominantly perinuclear distribution of lysosomes mediated by FUT1 knockdown as lysosomal positioning has been reported to regulate mTOR activity and autophagy. Taken together, our results suggest that downregulation of FUT1, which leads to the perinuclear localization of LAMP-1 and 2, is correlated with increased rate of autophagic flux by decreasing mTOR signaling and increasing autolysosome formation

A 42.3-43.6 GHz spectral survey of Orion BN/KL: First detection of the v=0 J=1-0 line from the isotopologues 29SiO and 30SiO

We have surveyed molecular line emission from Orion BN/KL from 42.3 to 43.6 GHz with the Green Bank Telescope. Sixty-seven lines were identified and ascribed to 13 different molecular species. The spectrum at 7 mm is dominated by SiO, SO2, CH3OCH3, and C2H5CN. Five transitions have been detected from the SiO isotopologues 28SiO, 29SiO, and 30SiO. We report here for the first time the spectra of the 29SiO and 30SiO v=0 J=1-0 emission in Orion BN/KL, and we show that they have double-peaked profiles with velocity extents similar to the main isotopologue. The main motivation for the survey was the search of high-velocity (100-1000 km/s) outflows in the BN/KL region as traced by SiO Doppler components. Some of the unidentified lines in principle could be high-velocity SiO features, but without imaging data their location cannot be established. Wings of emission are present in the v=0 28SiO, 29SiO and 30SiO profiles, and we suggest that the v=0 emission from the three isotopologues might trace a moderately high-velocity (~30-50 km/s) component of the flows around the high-mass protostar Source I in the Orion BN/KL region. We also confirm the 7 mm detection of a complex oxygen-bearing species, acetone (CH3COCH3), which has been recently observed towards the hot core at 3 mm, and we have found further indications of the presence of long cyanopolyynes (HC5N and HC7N) in the quiescent cold gas of the extended ridge.Comment: 27 pages, 3 figures, accepted by Ap

The Role of Thermodynamic Phase Shifts in the Extratropical Cloud Optical Depth Feedback

No abstract availabl

A preliminary study: Does relationship closeness with grandchildren correlate with the quality of life and physical health of Malaysian Chinese elderly?

The 13th Next-Generation Global Workshop第13回次世代グローバルワークショップテーマ: New Risks and Resilience in Asian Societies and the World 日程: 21-23 November, 2020 開催場所: ベトナム社会科学院(ハノイ)/Vietnam Academy of Social Sciences(No. 1 Lieu Giai street, Ba Dinh, Hanoi, Vietnam) ※Due to the COVID-19, the workshop will be held at ONLINE for overseas participants(not from Vietnam)/ONSITE for Vietnamese participants.This preliminary mixed-method study aimed to investigate whether grandparental childcare can contribute to elderly' quality of life and physical health. In the quantitative study, a total of 97 Chinese grandparents who are primary caregivers were recruited to examine the relations of relationship closeness, quality of life and physical health. Correlational analysis revealed that grandparent-grandchildren relationship closeness positively correlated with self-rated quality of life and physical health of the grandparents. In the qualitative interview, grandmothers (n = four Chinese grandmothers) who are primary caregivers were recruited to share their experience in taking care of grandchildren. Findings from the thematic analysis revealed that all grandmothers have a close relationship with their grandchildren. In specific, they are happy with the companionship of grandchildren even though they may feel physically tired in taking care of grandchildren. This preliminary study on skipped generation family provides insights into understanding the contribution of relationship closeness with grandchildren on the perceived quality of life and physical health of Malaysian grandparents who are the primary caregiver to their grandchildren

Evidence for nodeless superconducting gap in NaFe1−x_{1-x}Cox_xAs from low-temperature thermal conductivity measurements

The thermal conductivity of optimally doped NaFe$_{0.972}$Co$_{0.028}$As ($T_c \sim$ 20 K) and overdoped NaFe$_{0.925}$Co$_{0.075}$As ($T_c \sim$ 11 K) single crystals were measured down to 50 mK. No residual linear term $\kappa_0/T$ is found in zero magnetic field for both compounds, which is an evidence for nodeless superconducting gap. Applying field up to $H$ = 9 T ($\approx H_{c2}/4$) does not noticeably increase $\kappa_0/T$ in NaFe$_{1.972}$Co$_{0.028}$As, which is consistent with multiple isotropic gaps with similar magnitudes. The $\kappa_0/T$ of overdoped NaFe$_{1.925}$Co$_{0.075}$As shows a relatively faster field dependence, indicating the increase of the ratio between the magnitudes of different gaps, or the enhancement of gap anisotropy upon increasing doping.Comment: 5 pages, 4 figure

Contrasting the Co-Variability of Daytime Cloud and Precipitation over Tropical Land and Ocean

The co-variability of cloud and precipitation in the extended tropics (35 deg N35 deg S) is investigated using contemporaneous datasets for a 13-year period. The goal is to quantify potential relationships between cloud type amounts and precipitation events of particular strength. Particular attention is paid to whether the relationships exhibit different characteristics over tropical land and ocean. A primary analysis metric is the correlation coefficient between fractions of individual cloud types and frequencies within precipitation histogram bins that have been matched in time and space. The cloud type fractions are derived from Moderate Resolution Imaging Spectroradiometer (MODIS) joint histograms of cloud top pressure and cloud optical thickness in one-degree grid cells, and the precipitation frequencies come from the Tropical Rainfall Measuring Mission (TRMM) Multi-satellite Precipitation Analysis (TMPA) dataset aggregated to the same grid. It is found that the strongest coupling (positive correlation) between clouds and precipitation occurs over ocean for cumulonimbus clouds and the heaviest rainfall. While the same cloud type and rainfall bin are also best correlated over land compared to other combinations, the correlation magnitude is weaker than over ocean. The difference is attributed to the greater size of convective systems over ocean. It is also found that both over ocean and land the anti-correlation of strong precipitation with weak (i.e., thin and/or low) cloud types is of greater absolute strength than positive correlations between weak cloud types and weak precipitation. Cloud type co-occurrence relationships explain some of the cloud-precipitation anti-correlations. Weak correlations between weaker rainfall and clouds indicate poor predictability for precipitation when cloud types are known, and this is even more true over land than over ocean

DVI-SLAM: A Dual Visual Inertial SLAM Network

Recent deep learning based visual simultaneous localization and mapping (SLAM) methods have made significant progress. However, how to make full use of visual information as well as better integrate with inertial measurement unit (IMU) in visual SLAM has potential research value. This paper proposes a novel deep SLAM network with dual visual factors. The basic idea is to integrate both photometric factor and re-projection factor into the end-to-end differentiable structure through multi-factor data association module. We show that the proposed network dynamically learns and adjusts the confidence maps of both visual factors and it can be further extended to include the IMU factors as well. Extensive experiments validate that our proposed method significantly outperforms the state-of-the-art methods on several public datasets, including TartanAir, EuRoC and ETH3D-SLAM. Specifically, when dynamically fusing the three factors together, the absolute trajectory error for both monocular and stereo configurations on EuRoC dataset has reduced by 45.3% and 36.2% respectively.Comment: 7 pages, 3 figure

Method for comparing visual field defects to local RNFL and RGC damage seen on frequency domain OCT in patients with glaucoma.

To relate structural (anatomical) and functional (behavioral) measures of glaucomatous damage, a method is described for comparing visual field defects to local retinal ganglion cell (RGC) and retinal nerve fiber layer (RNFL) thicknesses. Thickness maps of the RNFL and the RGC + inner plexiform layer, obtained with frequency domain optical coherence tomography (fdOCT), were transformed into probability maps by comparing them to a normative group. As demonstrated in patients with glaucomatous damage to the macula, the probability map associated with the patient’s visual field can be directly compared to the fdOCT probability maps by taking into consideration the displacement of the RGCs