Los procesos de evaluación de la calidad en los estudios de enfermería en el contexto del espacio europeo de educación superior

El objetivo de este artículo es realizar una aproximación sobre el desarrollo de la evaluación institucional en nuestro país, nuevos programas y retos que se están planteando en las titulaciones. La convergencia europea tiene en los procesos de calidad de los programas de formación y de los centros uno de los pilares más importantes. En Europa, incluida España, se han desarrollado en los últimos años intensos procesos de evaluación de la calidad propiciando la introducción de mejoras y la rendición de cuentas a la sociedad

Peritoneal fluid reduces angiogenesis-related microRNA expression in cell cultures of endometrial and endometriotic tissues from women with endometriosis.

UNLABELLED: Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent gynecological diseases. It has been suggested that modifications of both endometrial and peritoneal factors could be implicated in this disease. Endometriosis is a multifactorial disease in which angiogenesis and proteolysis are dysregulated. MicroRNAs (miRNAs) are small non-coding RNAs that regulate the protein expression and may be the main regulators of angiogenesis. Our hypothesis is that peritoneal fluid from women with endometriosis could modify the expression of several miRNAs that regulate angiogenesis and proteolysis in the endometriosis development. The objective of this study has been to evaluate the influence of endometriotic peritoneal fluid on the expression of six miRNAs related to angiogenesis, as well as several angiogenic and proteolytic factors in endometrial and endometriotic cell cultures from women with endometriosis compared with women without endometriosis. METHODS: Endometrial and endometriotic cells were cultured and treated with endometriotic and control peritoneal fluid pools. We have studied the expression of six miRNAs (miR-16, -17-5p, -20a, -125a, -221, and -222) by RT-PCR and protein and mRNA levels of vascular endothelial growth factor-A, thrombospondin-1, urokinase plasminogen activator and plasminogen activator inhibitor-1 by ELISA and qRT-PCR respectively. RESULTS: Control and endometriotic peritoneal fluid pools induced a significant reduction of all miRNAs levels in endometrial and endometriotic cell cultures. Moreover, both peritoneal fluids induced a significant increase in VEGF-A, uPA and PAI-1 protein levels in all cell cultures without significant increase in mRNA levels. Endometrial cell cultures from patients treated with endometriotic peritoneal fluid showed lower expression of miRNAs and higher expression of VEGF-A protein levels than cultures from controls. In conclusion , this "in vitro" study indicates that peritoneal fluid from women with endometriosis modulates the expression of miRNAs that could contribute to the angiogenic and proteolytic disequilibrium observed in this disease

La evaluación como instrumento de aprendizaje : técnicas y estrategias

Este volumen tiene su origen en el curso de formación del profesorado: La evaluación como instrumento de aprendizaje. Técnicas y estrategias. Segovia, 2006La evaluación está en constante punto de mira y es uno de los mayores desafíos a los que se enfrentan los profesionales de la educación en la sociedad actual. Para concebir y practicar una evaluación como instrumento de aprendizaje es primordial que el profesorado afiance una cultura evaluativa eminentemente pedagógica y educativa, lo que supone, por encima de todo, asumir la evaluación como: ayuda al alumnado a aprender mejor y a ser cada vez más autónomo; recurso de investigación, formación y desarrollo profesional y personal del docente; garantía de calidad para el centro educativo. Está dirigida al profesorado de todas las etapas educativas y ofrece argumentos para la reflexión y recursos para la práctica.MadridBiblioteca de Educación del Ministerio de Educación, Cultura y Deporte; Calle San Agustín 5 -3 Planta; 28014 Madrid; Tel. +34917748000; [email protected]

Peritoneal fluid (PF) effects on VEGF-A and TSP-1 expression in stromal cell cultures from control endometrial tissue, and patient endometrial and endometriotic tissues from women with endometriosis.

<p>ØPF, without PF; CPF, control PF; EPF, endometriotic PF. Data are expressed as Mean ± SEM. * <i>p</i><0.05; ** <i>p</i><0.01; *** <i>p</i><0.001; vs ØPF from same tissue culture, ψ <i>p</i><0.05 vs ØPF from control endometrium culture. A: VEGF-A protein; B: VEGF-A mRNA; C: TSP-1 protein; D: TSP-1 mRNA.</p

Peritoneal fluid (PF) effects on miRNA expression in stromal cell cultures from control endometrial tissue, and patient endometrial and endometriotic tissues from women with endometriosis.

<p>ØPF, without PF; CPF, control PF; EPF, endometriotic PF. *<i>p</i><0.05; **<i>p</i><0.01; <i>p</i><0.001 vs ØPF same tissue culture. miRNA expression is presented as fold change relative to control stromal cell culture without peritoneal fluid (control endometrium ØPF = 1). A: miR-16; B: miR-17-5p; C: miR-20a; D: miR-125a; E: miR-221; F: miR-222.</p

Correlation between changes in miR-16 vs. VEGF-A protein levels in eutopic endometrial (A) and endometriotic cell cultures (B) from women with endometriosis after treatment with both peritoneal fluid pools compared to cell cultures without peritoneal fluid treatment.

<p>Change: differences between parameter levels with and without peritoneal fluid treatment.</p

Peritoneal fluid (PF) effects on uPA and PAI-1 expression in stromal cell cultures from control endometrial tissue, and patient endometrial and endometriotic tissues from women with endometriosis.

<p>ØPF, without PF; CPF, control PF; EPF, endometriotic PF. Data are expressed as Mean ± SEM. * <i>p</i><0.05; ** <i>p</i><0.01; *** <i>p</i><0.001; vs ØPF from same tissue culture, ψ <i>p</i><0.05 vs ØPF from control endometrium culture. A: uPA protein; B: uPA mRNA; C: PAI-1 protein; D: PAI-1 mRNA.</p