PHYTOCHEMICAL INVESTIGATIONS AND ANTIMICROBIAL AND ANTICANCER ACTIVITIES OF HOMONOIA RIPARIA LOUR

Objective: Present study aims at the evaluation of antibacterial activity against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Bacillus cereus, antifungal activity against Trichophyton mentagrophytes and T. rubrum and anticancer activity against human lung cancer cell line - A549 of extracts of Homonoia riparia Lour. The extracts showing antimicrobial activity were further subjected to phytochemical analysis to determine the major group of compounds present.Methods: The procedures used for testing the antimicrobial and anticancer activity were as per the prescribed methods.Results: DMSO extracts of male flowers inhibited growth of P. vulgaris and S. aureus whereas, aqueous extracts of both leaves and male flowers inhibited growth of T. mentagrophytes. Phytochemical analysis of these extracts showed the presence of tannins, saponins and flavonoids in leaves and flowers. Tannins, saponins and flavonoids were separated by traditional extractive methods. MIC of tannins from flowers was 50 mg ml-1against P. vulgaris and of tannins and flavonoids was 40 mg ml-1against S. aureus. The extracted tannins, flavonoids, saponins and steroids and triterpenoids from leaves and flowers inhibited the growth of T. mentagrophytes. TLC of these compounds showed presence of a few common components in the leaves and flowers. The ethanolic extracts of H. riparia did not exhibit anticancer activity.Conclusion: The leaves and male flowers of H. riparia possess antimicrobial activity. The tannins, flavonoids, saponins, steroids and triterpenoids are present in leaves and flowers and inhibit the growth of P. vulgaris, S. aureus and T. mentagrophytes

Evaluation of ethanolic and aqueous extract of Clitoria ternatea for antimicrobial activity

194-198In the recent years, there has been an emerge and spread of infectious diseases and also developing resistance to the antibiotics, so there is a great concern to find potentially effective, safer natural alternative for the infectious diseases. The present study was focused on the antimicrobial activity of ethanol and aqueous extract of Clitoria ternatea root. The in-vitro antimicrobial study was carried out against bateriea viz. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and fungus viz. Aspergilus niger, Candida albicans by the cup-plate method. The extracts of Clitoria ternatea roots were subjected to preliminary phytochemical screening for detection of chemical constituents present in them. Preliminary phytochemical screening study revealed the presence of alkaloids, glycosides, tannins, phenol and flavonoids. The highest potential was observed in the ethanol extract of Clitoria ternatea roots. This study suggested that solvent polarity determined the phenol and flavonoid content significantly, hence affecting the antimicrobial activity. This plant extract which proved to be potentially effective can be used as a natural alternative for preventives to control infectious diseases causing due to the tested microorganisms

Evaluation of ethanolic and aqueous extract of Clitoria ternatea for antimicrobial activity

In the recent years, there has been an emerge and spread of infectious diseases and also developing resistance to the antibiotics, so there is a great concern to find potentially effective, safer natural alternative for the infectious diseases. The present study was focused on the antimicrobial activity of ethanol and aqueous extract of Clitoria ternatea root. The in-vitro antimicrobial study was carried out against bateriea viz. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and fungus viz. Aspergilus niger, Candida albicans by the cup-plate method. The extracts of Clitoria ternatea roots were subjected to preliminary phytochemical screening for detection of chemical constituents present in them. Preliminary phytochemical screening study revealed the presence of alkaloids, glycosides, tannins, phenol and flavonoids. The highest potential was observed in the ethanol extract of Clitoria ternatea roots. This study suggested that solvent polarity determined the phenol and flavonoid content significantly, hence affecting the antimicrobial activity. This plant extract which proved to be potentially effective can be used as a natural alternative for preventives to control infectious diseases causing due to the tested microorganisms

An Eco-benign Synthesis of AgNps using Hydroalcoholic Extract of Brassica oleracea var. italica Plenck : Anticancer Response against Human Breast Cancer Cells MCF-7

Breast Cancer is the second foremost reason of Cancer mortality in females globally. The conventional treatments available for Breast Cancer include surgery yet they are related to serious side effects that have moved the worldwide focus towards Complementary and Alternative Medicines (CAM). One of the emerging strategies has been the use of plant extracts for synthesizing metal nanoparticles (such as gold and silver) for anticancer applications. The objective of this study is to reflect the current availed study on green synthesis of silver nanoparticles (AgNps) with its future prospects to treat Breast Cancer. The development of eco-friendly and reliable techniques for silver nanoparticles synthesis is a vital initiative in the area of nanotechnology. Brassica oleracea var. italica Plenck Leaves Extract (LE) prepared by maceration process and silver nanoparticles of LE prepared by using biological reduction method. Female rats were divided into 5 groups, Group-I served as Positive Control and received normal saline. Group-II served Negative Control (Tumor Bearing) and was treated with single dose of MCF 7. Breast Cancer Cells (1.7 mg/pellet). Group-III served Standard Control (Tumor Bearing) treated with Paclitaxel 40 mg/Kg. Group-IV served test and treated with LE 400 mg/Kg, Group-V served test and treated with LENP 400 mg/Kg respectively about 21 days and on 21th day blood samples were collected for Hematological Parameters and Feed and Water Consumption, Body Weight Determination and Organ Weight were estimated. These discoveries infer that the synthesized Silver nanoparticles utilizing green nanotechnology could be a perfect methodology to battle malignant growth and irresistible ailments. Keywords:  Breast Cancer, Nanotechnology, Synthesis, , MCF-7, CA