Objective: Present study aims at the evaluation of antibacterial activity against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Bacillus cereus, antifungal activity against Trichophyton mentagrophytes and T. rubrum and anticancer activity against human lung cancer cell line - A549 of extracts of Homonoia riparia Lour. The extracts showing antimicrobial activity were further subjected to phytochemical analysis to determine the major group of compounds present.Methods: The procedures used for testing the antimicrobial and anticancer activity were as per the prescribed methods.Results: DMSO extracts of male flowers inhibited growth of P. vulgaris and S. aureus whereas, aqueous extracts of both leaves and male flowers inhibited growth of T. mentagrophytes. Phytochemical analysis of these extracts showed the presence of tannins, saponins and flavonoids in leaves and flowers. Tannins, saponins and flavonoids were separated by traditional extractive methods. MIC of tannins from flowers was 50 mg ml-1against P. vulgaris and of tannins and flavonoids was 40 mg ml-1against S. aureus. The extracted tannins, flavonoids, saponins and steroids and triterpenoids from leaves and flowers inhibited the growth of T. mentagrophytes. TLC of these compounds showed presence of a few common components in the leaves and flowers. The ethanolic extracts of H. riparia did not exhibit anticancer activity.Conclusion: The leaves and male flowers of H. riparia possess antimicrobial activity. The tannins, flavonoids, saponins, steroids and triterpenoids are present in leaves and flowers and inhibit the growth of P. vulgaris, S. aureus and T. mentagrophytes
