Antifouling Zwitterionic Nanofibrous Wound Dressing for Long-Lasting Antibacterial Photodynamic Therapy

Nanofibrous mats as a wound dressing have received great attention in recent year. The development of biocompatible dressings with antibiofouling capability and long-lasting antibacterial properties is important but challenging. Antibacterial photodynamic therapy (aPDT) effectively eliminates pathogens via a photodynamic process that can circumvent the emergence of antibiotic-resistant pathogens. In this study, we integrated the zwitterionic materials (2-methacryloyloxyethyl phosphorylcholine (MPC) moiety) and aPDT photosensitizer, methylene blue (MB), to fabricate a long-lasting antibacterial nanofibrous mat using electrospinning technology. The prepared nanofibers possessed an appropriate water absorption and retention ability, superior cytocompatibility, and antibiofouling ability against both proteins and L929 cell adhesion. MB-loaded nanofibrous mats have exhibited superior aPDT against Gram-positive Staphylococcus aureus compared to Gram-negative Escherichia coli under moderate irradiation (100 W m–2) due to the presence of an extra outer membrane of Gram-negative bacteria serving as a protective barrier. In vitro release study demonstrated that the nanofibrous mat had a long-lasting drug release profile, which can efficiently suppress bacterial growth via aPDT. The antibacterial ability of the MB-loaded nanofibrous mat was commensurate or slightly inferior to antibiotics such as tetracycline and kanamycin, suggesting that it has the potential to be used as an antibiotic alternative. Overall, this zwitterionic nanofibrous mat with long-lasting aPDT function and nonadherent properties has potential as a promising antibacterial wound dressing

Underwater visual census_Plant II

Underwater visual census_Plant I

Reference of species

Reference of specie

Trammel net sampling_Plant II

Trammel net sampling_Plant I

Trammel net sampling_Plant I

Trammel net sampling_Plant

README

READM

Underwater visual census_Plant I

Underwater visual census_Plant

Adiponectin receptor 1 resists the decline of serum osteocalcin and GPRC6A expression in ovariectomized mice

<div><p>Hormonal changes that cause metabolic complications are a common problem in postmenopausal women. Adiponectin and osteocalcin are cytokines associated with glucose regulatory and insulin sensitized function in postmenopausal stages. The current study investigated the role of adiponectin signaling and osteocalcin mediated function in glucose metabolism in ovariectomized mice. In a mouse menopausal-related metabolic disorder model, overexpression of adiponectin receptor 1 improved glucose tolerance and caused resistance to body weight increase and decline of serum osteocalcin. Furthermore, adiponectin receptor 1 transgenic ovariectomized mice had higher GPRC6A (the putative osteocalcin receptor) expression in muscle tissue. Immunofluorescence indicated that GPRC6A and adiponectin receptor 1 were co-localized in mouse muscle tissues. The present finding suggested adiponectin receptor 1 can mediate the improvement of glucose metabolism by osteocalcin in ovariectomized mice. Our findings imply the possibility to ameliorate menopause-induced metabolic disorder by GPRC6A and adiponectin signaling.</p></div

Immunofluorescence stain of GPRC6A and AdipoR1 in mouse muscle tissue.

<p>Cell nulei were stained with DAPI (blue). Muscle tissue sections were stained with GPRC6A (red) and AdipoR1 (green) antibody and viewed at X400 magnification with scale bars indicating 20 mm. Arrow refers to the co-localization of GPRC6A and AdipoR1.</p

Impinged fish dataset 1987-1990

Impinged fish dataset 1987-199