615 research outputs found
Induction of HSPA4 and HSPA14 by NBS1 overexpression contributes to NBS1-induced in vitro metastatic and transformation activity
<p>Abstract</p> <p>Background</p> <p>Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome associated with cancer predisposition, radiosensitivity, microcephaly, and growth retardation. The NBS gene product, NBS1 (p95) or nibrin, is a part of the MRN complex, a central player associated with double-strand break (DSB) repair. We previously demonstrated that NBS1 overexpression contributes to transformation through the activation of PI 3-kinase/Akt. NBS1 overexpression also induces epithelial-mesenchymal transition through the Snail/MMP2 pathway.</p> <p>Methods</p> <p>RT-PCR, Western blot analysis, <it>in vitro </it>migration/invasion, soft agar colony formation, and gelatin zymography assays were performed.</p> <p>Results</p> <p>Here we show that heat shock protein family members, A4 and A14, were induced by NBS1 overexpression. siRNA mediated knockdown of HSPA4 or HSPA14 decreased the <it>in vitro </it>migration, invasion, and transformation activity in H1299 cells overexpressing NBS1. However, HSPA4 or HSPA14 induced activity was not mediated through MMP2. NBS1 overexpression induced the expression of heat shock transcription factor 4b (HSF4b), which correlated with the expression of HSPA4 and HSPA14.</p> <p>Conclusion</p> <p>These results identify a novel pathway (NBS1-HSF4b-HSPA4/HSPA14 axis) to induce migration, invasion, and transformation, suggesting the activation of multiple signaling events induced by NBS1 overexpression.</p
Designing an Adaptive Assisting Interface for Learning Virtual Filmmaking
International audienceIn this paper, we present an adaptive assisting interface for learning virtual filmmaking. The design of the system is based on the scaffolding theory, to provide timely guidance to the user in the form of visual and audio messages that are adapted to each person's skill level and performance. The system was developed on an existing virtual filmmaking setup. We conducted a study with 24 participants, who were asked to operate the film set with or without our adaptive assisting interface. Results suggest that our system can provide users with a better learning experience and positive knowledge harvest
Smoking, Habitual Tea Drinking and Metabolic Syndrome in Elderly Men Living in Rural Community: The Tianliao Old People (TOP) Study 02
The literature shows an inconsistent relationship between lifestyle behaviors and metabolic syndrome (MetS), especially in the elderly. We designed this study to investigate the interrelationships among cigarette smoking, tea drinking and MetS, and to verify the factors associated with MetS in elderly males dwelling in rural community. In July 2010, with a whole community sampling method, 414 male subjects aged over 65 dwelling in Tianliao township were randomly sampled. The response rate was 60.8%. Each subject completed the structured questionnaires including sociodemographic characteristics, habitual behaviors (including cigarette smoking and tea drinking habits) and medical history. After an overnight fast, the laboratory and anthropometric data were obtained. MetS was confirmed according to the criteria defined by the modified NCEP ATP III for the male Chinese population. Subjects were split into either non-MetS or MetS groups for further analysis. Of the 361 subjects with complete data, 132 (36.6%) elderly men were classified as having MetS. Using binary logistic regression, body mass index, serum uric acid, high sensitivity C-reactive protein, HOMA index, current smokers (OR = 2.72, 95%CI: 1.03 ∼ 7.19), total smoking amount > = 30 (OR = 2.78, 95%CI: 1.31 ∼ 5.90) and more than 20 cigarettes daily (OR = 2.54, 95%CI: 1.24 ∼ 5.18) were positively associated with MetS. Current un- or partial fermented tea drinker (OR = 0.42, 95%CI: 0.22 ∼ 0.84), tea drinking habit for 1–9 years (OR = 0.36, 95%CI: 0.15 ∼ 0.90) and more than 240cc daily (OR = 0.35, 95%CI: 0.17 ∼ 0.72) were negatively associated with MetS. In conclusion, this study suggests that smoking habit was positively associated with MetS, but tea drinking habit was negatively associated with MetS in elderly men dwelling in rural community
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Acceptor plane expansion enhances horizontal orientation of thermally activated delayed fluorescence emitters.
Manipulating orientation of organic emitters remains a formidable challenge in organic light-emitting diodes (OLEDs). Here, expansion of the acceptor plane of thermally activated delayed fluorescence (TADF) emitters was demonstrated to selectively modulate emitting dipole orientation. Two proof-of-the-concept molecules, PXZPyPM and PXZTAZPM, were prepared by introducing a planar 2-phenylpyridine or 2,4,6-triphenyl-1,3,5-triazine substituent into a prototypical molecule (PXZPM) bearing a pyrimidine core and two phenoxazine donors. This design approach suppressed the influence of substituents on electronic structures and associated optoelectronic properties. Accordingly, PXZPyPM and PXZTAZPM preserved almost the same excited states and similar emission characteristics as PXZPM. The expanded acceptor plane of PXZPyPM and PXZTAZPM resulted in a 15 to 18% increase in horizontal ratios of emitting dipole orientation. PXZPyPM supported its green device exhibiting an external quantum efficiency of 33.9% and a power efficiency of 118.9 lumen per watt, competitive with the most efficient green TADF OLEDs reported so far
Wafer-scale growth of large arrays of perovskite microplate crystals for functional electronics and optoelectronics
Methylammonium lead iodide perovskite has attracted intensive interest for
its diverse optoelectronic applications. However, most studies to date have
been limited to bulk thin films that are difficult to implement for integrated
device arrays because of their incompatibility with typical lithography
processes. We report the first patterned growth of regular arrays of perovskite
microplate crystals for functional electronics and optoelectronics. We show
that large arrays of lead iodide microplates can be grown from an aqueous
solution through a seeded growth process and can be further intercalated with
methylammonium iodide to produce perovskite crystals. Structural and optical
characterizations demonstrate that the resulting materials display excellent
crystalline quality and optical properties. We further show that perovskite
crystals can be selectively grown on prepatterned electrode arrays to create
independently addressable photodetector arrays and functional field effect
transistors. The ability to grow perovskite microplates and to precisely place
them at specific locations offers a new material platform for the fundamental
investigation of the electronic and optical properties of perovskite materials
and opens a pathway for integrated electronic and optoelectronic systems.Comment: 8 pages, 4 figure
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