<i>Pelagia noctiluca</i> sampling information.

<p>The table displays sampling sites and collection years, the population sample acronym, the number of individuals and the sampling coordinates.</p

Pairwise F_ST and respective p-values.

<p>F_ST values for the original uncorrected dataset calculated with Arlequin ver. 3.5.1.3 (Excoffier and Lischer 2010). F_ST values are reported below the diagonal, while p-values are shown above the diagonal. In bold, significant values, after Bonferroni correction (α = 0.00179).</p

F_IS values calculated with and without null alleles correction.

<p>Locus by locus and over all loci F_IS values were calculated for the original and the corrected dataset after Microchecker v. 2.2.3 analysis.</p><p>n.e.: Not evaluated due to HWE and no changes in the corrected dataset.</p

Summary of genetic variability for all <i>Pelagia noctiluca</i> samples.

<p>Eight sampling sites at nine microsatellite loci are described, including total number of alleles (NA), observed (Ho), expected heterozygosity (He) and probabilities of deviation from Hardy–Weinberg equilibrium (HWE).</p>a<p>Values in bold indicate significant HWE deviations (α = 0.05).</p

General information about microsatellite markers in <i>Pelagia noctiluca</i>.

a<p>For each locus, the upper sequence refers to the forward primer, the lower sequence refers to the reverse primer.</p>b<p>Label at the 5′– end of the forward primers.</p>c<p>Loci showing the same number were amplified together in the same multiplex PCR.</p

16S sequences for calibration

The 16S fish sequences used in the 16S molecular cloc

Traits of the 334 specimens

A list of all 334 specimens of Periophthalmus argentilineatus, Periophthalmus kalolo and of the outgroup P. malaccensis. For each call number that is used in the nexus files, the species, the sample locality and the population in which the specimen was included by the AMOVA in the paper, are indicated

Summary of SSR types and frequency in <i>Chamelea gallina</i> transcriptome.

<p>Summary of SSR types and frequency in <i>Chamelea gallina</i> transcriptome.</p

Variability assessment of 14 SSR loci of <i>Chamelea gallina</i>.

<p>Variability, expressed in terms of number of different alleles, was assessed on 12 individuals collected in Chioggia in 2010 (off Venice lagoon, Italy). The table reports the name of each locus, taken from the contig number, the repeat content, the forward (F) and reverse (R) primer sequences, the fluorescent label, the annealing temperature (Ta) of PCR amplification, the size range of amplified fragments in bp, the allelic range in repeats, the number of alleles (Na) detected and the Hardy-Weinberg probability (pHWE). Significant p-values in bold (α = 0.05). Mean values for allele number, observed and expected heterozygosity are reported in the last row. Standard Deviation is reported in brackets (± SD).</p>*<p>Loci putatively affected by null alleles following MICRO-CHECKER 2.2.3. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044185#pone.0044185-vanOosterhout1" target="_blank">[51]</a>.</p>a<p>p-values were calculated based on a limited number of individuals (n = 12).</p

<i>Chamelea gallina</i> SSR mining results.

<p>The table reports the main results provided by MISA for SSR detection.</p