Purification and characterization of angiotensin-1 converting enzyme (ACE)-inhibitory peptide from the jellyfish, Nemopilema nomurai

The Nemopilema nomurai hydrolysate was produced by the reaction of papain, and an angiotensin-Ι converting enzyme (ACE)-inhibitory peptide was purified by using the molecular cut-offs membrane filter, the gel filtration chromatography with Sephadex LH-20 and the reverse phase chromatographic method using C18 and C12 columns. Purification yield of the active peptide was estimated to be 0.2 ± 0.1%, starting from the lyophilized jellyfish. The infrared (IR), proton nuclear magnetic resonance spectroscopy (1H NMR), carbon nuclear magnetic resonance (13C NMR) and mass spectrometry (MS) spectrometer analyses elucidated that the structure of the purified peptide is tyrosine-isoleucine (Tyr-Ile). The inhibitory concentration at 50% (IC50) and Ki values were calculated to be 2.0 ± 0.3 μg/ml and 3.3 ± 0.3 μM, respectively, which acts as a competitive inhibitor to ACE.Keywords: Angiotensin-Ι converting enzyme, Jellyfish, Nemopilema nomurai, Papain hydrolysate, Tyrosine-IsoleucineAfrican Journal of Biotechnology Vol. 12(15), pp. 1888-189

Effects of Glycyrrhizae Radix Pharmacopuncture Intravenous Injection on Ischemia-induced Acute Renal Failure in Rabbits

Objectives: The present study was undergone to determine whether Glycyrrhizae Radix pharmacopuncture intravenous injection exerts beneficial effect against the ischemia-induced acute renal failure in rabbits. Methods: Rabbits were treated with Glycyrrhizae Radix pharmacopuncture via i.v., followed by renal ischemia/reperfusion. The fractional excretion of glucose and phosphate were measured and the malondialdehyde content was also determined. The morphological changes of cortical part of kidney also observed with light microscope. Results: Renal ischemia/reperfusion caused increase of the fractional excretion of glucose and phosphate in ischemia-induced animals, which was prevented by Radix Glycyrrhizae extract treatment. Ischemia/reperfusion increased lipid peroxidation, which was prevented and morphological changes also altered by Radix Glycyrrhizae pharmacopuncture administration. Conclusions: These results indicate that lipid peroxidation plays a critical role in ischemia-induced acute renal failure and Glycyrrhizae Radix pharmacopuncture exerts the protective effect against acute renal failure induced by renal ischemia/reperfusion

염색체 이상 증후군의 병리학적 검색

To determine the structural anomalies associated in various chromosomal aberration syndromes we have analyzed postmorm findings of 18 autopsies with chromosomal anomalies that were collected during the last 14 years, 1975-1988. The diagnosis was confirmed by karyotyping in 14 cases and characteristic sets of anomalies in 4 cases. The cases consisted of 10 cases of Edwards syndrome, 5 cases of Down syndrome, 2 cases of Patau syndrome and one case of 4p-syndrome. Four cases were stillborns and 14 cases survived from a few hours to months. There were 3 males and 15 females. Cardiovascular malformations were common. They were patent ductus arteriosus, atrial septal defect and ventricular septal defect. They were particularly common in Down and Edwards syndrome. Among respiratory anomalies, abnormal lobation was common. Tracheo-esophageal fistula and lung hypoplasia were also seen in some cases. They were particularly common in Edwards syndrome. Gastrointestinal malformations were also frequently encountered in Edwards syndrome. They were diaphragmatic hernia, Meckel's diverticulum and mobile intestine. Genitoruinary malformation consisted of cystic dysplasia, horseshoe kidney and hypoplasia of gonads. These anomalies were also common in Edwards syndrome. Central nervous system anomalies were common in Patau syndrome. Holotelencephaly and microcephaly were common. Other skeletal and characteristic facial anomalies were also seen in these malformation sydrome. It was concluded that a fairly characteristic constellation of structural defects were associated with specific type of chromosomal abnormalities. Therefore these sets of findings could be applied as diagnostic criteria when chromosomal analysis is not available or fails

단지흥을 특징으로 하는 선천성 골격 이형성증(14부검례의 형태학적 분석)

We examined the clinico-pathologic features and radiographic findings of a total of 14 qutopsy cases of congenital short-limbed dwarfism in an attempt to identify the morphological features of diagnostic significance. They included one case each of achondrogenesis type I, achondroplasia, short rib-polydactyly syndrome, chondrodystrophia calcificans congenita, and 3 cases of thanatophoric dwarfism, and 8 cases of osteogenesis imperfecta fetalis. All cases were born dead or died in a first few days of life because of respiratory distress produced by short and narrow thoraces. Birth weights were generally low for their gestational age. A striking feature was the discrepancy between the relatively long, narrow trunk and the extremely short extremities. They had a relatively large head with frontal bossing, and flattened nasal bridge. The pathological observation of these materials demonstrated that the majority of chondrodysplasia were distinct abnormalities in the endochondral osification. There were subtle but definite differences in the degree of involvement of endochondral ossification. We presented clinical, radiological and morphologic features observed in six different types of osteochondrodysplasia and discussed characteristic differential features of congenital short limbed dwarfism