RNA processing by the CRISPR-associated NYN ribonuclease

Funding: EC | European Research Council (ERC) - 101018608; China Scholarship Council (CSC) - 202008420207.CRISPR-Cas systems confer adaptive immunity in prokaryotes, facilitating the recognition and destruction of invasive nucleic acids. Type III CRISPR systems comprise large, multisubunit ribonucleoprotein complexes with a catalytic Cas10 subunit. When activated by the detection of foreign RNA, Cas10 generates nucleotide signalling molecules that elicit an immune response by activating ancillary effector proteins. Among these systems, the Bacteroides fragilis type III CRISPR system was recently shown to produce a novel signal molecule, SAM-AMP, by conjugating ATP and SAM. SAM-AMP regulates a membrane effector of the CorA family to provide immunity. Here, we focus on NYN, a ribonuclease encoded within this system, probing its potential involvement in crRNA maturation. Structural modelling and in vitro ribonuclease assays reveal that NYN displays robust sequence-nonspecific, Mn2+-dependent ssRNA-cleavage activity. Our findings suggest a role for NYN in trimming crRNA intermediates into mature crRNAs, which is necessary for type III CRISPR antiviral defence. This study sheds light on the functional relevance of CRISPR-associated NYN proteins and highlights the complexity of CRISPR-mediated defence strategies in bacteria.Peer reviewe

e-SAFE: Secure, Efficient and Forensics-Enabled Access to Implantable Medical Devices

To facilitate monitoring and management, modern Implantable Medical Devices (IMDs) are often equipped with wireless capabilities, which raise the risk of malicious access to IMDs. Although schemes are proposed to secure the IMD access, some issues are still open. First, pre-sharing a long-term key between a patient's IMD and a doctor's programmer is vulnerable since once the doctor's programmer is compromised, all of her patients suffer; establishing a temporary key by leveraging proximity gets rid of pre-shared keys, but as the approach lacks real authentication, it can be exploited by nearby adversaries or through man-in-the-middle attacks. Second, while prolonging the lifetime of IMDs is one of the most important design goals, few schemes explore to lower the communication and computation overhead all at once. Finally, how to safely record the commands issued by doctors for the purpose of forensics, which can be the last measure to protect the patients' rights, is commonly omitted in the existing literature. Motivated by these important yet open problems, we propose an innovative scheme e-SAFE, which significantly improves security and safety, reduces the communication overhead and enables IMD-access forensics. We present a novel lightweight compressive sensing based encryption algorithm to encrypt and compress the IMD data simultaneously, reducing the data transmission overhead by over 50% while ensuring high data confidentiality and usability. Furthermore, we provide a suite of protocols regarding device pairing, dual-factor authentication, and accountability-enabled access. The security analysis and performance evaluation show the validity and efficiency of the proposed scheme

Intelligent Perception Control System of Railway Level Crossing Gate Based on TRIZ Theory

TRIZ theory is an innovative method to analyse problems and solve them, which is widely used in many fields. In this paper, TRIZ theory is used to improve the design of railway crossing guardrail system. The use of nine-screen analysis, functional analysis, cause-effect chain analysis and other tools to analyse the problem of poor manual control effect in the railway crossing guardrail system, the use of technical contradictions, physical contradictions and other tools to improve the system design, effectively reduce the possibility of danger when cars and pedestrians cross railway crossings, improve the traffic safety and traffic order of the railway level crossing, and reduce the work burden of railway crossing caretakers

KD-MVS: Knowledge Distillation Based Self-supervised Learning for Multi-view Stereo

Supervised multi-view stereo (MVS) methods have achieved remarkable progress in terms of reconstruction quality, but suffer from the challenge of collecting large-scale ground-truth depth. In this paper, we propose a novel self-supervised training pipeline for MVS based on knowledge distillation, termed KD-MVS, which mainly consists of self-supervised teacher training and distillation-based student training. Specifically, the teacher model is trained in a self-supervised fashion using both photometric and featuremetric consistency. Then we distill the knowledge of the teacher model to the student model through probabilistic knowledge transferring. With the supervision of validated knowledge, the student model is able to outperform its teacher by a large margin. Extensive experiments performed on multiple datasets show our method can even outperform supervised methods

Privacy Leakage in Smart Homes and Its Mitigation: IFTTT as a Case Study

The combination of smart home platforms and automation apps introduces much convenience to smart home users. However, this also brings the potential for privacy leakage. If a smart home platform is permitted to collect all the events of a user day and night, then the platform will learn the behavior patterns of this user before long. In this paper, we investigate how IFTTT, one of the most popular smart home platforms, has the capability of monitoring the daily life of a user in a variety of ways that are hardly noticeable. Moreover, we propose multiple ideas for mitigating privacy leakages, which altogether forms a Filter-and-Fuzz (F&F) process: first, it filters out events unneeded by the IFTTT platform; then, it fuzzes the values and frequencies of the remaining events. We evaluate the F&F process, and the results show that the proposed solution makes IFTTT unable to recognize any of the user's behavior patterns

Weight-dependent Gates for Differentiable Neural Network Pruning

In this paper, we propose a simple and effective network pruning framework, which introduces novel weight-dependent gates to prune filter adaptively. We argue that the pruning decision should depend on the convolutional weights, in other words, it should be a learnable function of filter weights. We thus construct the weight-dependent gates (W-Gates) to learn the information from filter weights and obtain binary filter gates to prune or keep the filters automatically. To prune the network under hardware constraint, we train a Latency Predict Net (LPNet) to estimate the hardware latency of candidate pruned networks. Based on the proposed LPNet, we can optimize W-Gates and the pruning ratio of each layer under latency constraint. The whole framework is differentiable and can be optimized by gradient-based method to achieve a compact network with better trade-off between accuracy and efficiency. We have demonstrated the effectiveness of our method on Resnet34, Resnet50 and MobileNet V2, achieving up to 1.33/1.28/1.1 higher Top-1 accuracy with lower hardware latency on ImageNet. Compared with state-of-the-art pruning methods, our method achieves superior performance.Comment: ECCV worksho

Ray Denoising: Depth-aware Hard Negative Sampling for Multi-view 3D Object Detection

Multi-view 3D object detection systems often struggle with generating precise predictions due to the challenges in estimating depth from images, increasing redundant and incorrect detections. Our paper presents Ray Denoising, an innovative method that enhances detection accuracy by strategically sampling along camera rays to construct hard negative examples. These examples, visually challenging to differentiate from true positives, compel the model to learn depth-aware features, thereby improving its capacity to distinguish between true and false positives. Ray Denoising is designed as a plug-and-play module, compatible with any DETR-style multi-view 3D detectors, and it only minimally increases training computational costs without affecting inference speed. Our comprehensive experiments, including detailed ablation studies, consistently demonstrate that Ray Denoising outperforms strong baselines across multiple datasets. It achieves a 1.9\% improvement in mean Average Precision (mAP) over the state-of-the-art StreamPETR method on the NuScenes dataset. It shows significant performance gains on the Argoverse 2 dataset, highlighting its generalization capability. The code will be available at https://github.com/LiewFeng/RayDN