A Multimodal Scaffold for SDF1 Delivery Improves Cardiac Function in a Rat Subacute Myocardial Infarct Model

Ischemic heart disease is one of the leading causes of death worldwide. The efficient delivery of therapeutic growth factors could counteract the adverse prognosis of post-myocardial infarction (post-MI). In this study, a collagen hydrogel that is able to load and appropriately deliver pro-angiogenic stromal cell-derived factor 1 (SDF1) was physically coupled with a compact collagen membrane in order to provide the suture strength required for surgical implantation. This bilayer collagen-on-collagen scaffold (bCS) showed the suitable physicochemical properties that are needed for efficient implantation, and the scaffold was able to deliver therapeutic growth factors after MI. In vitro collagen matrix biodegradation led to a sustained SDF1 release and a lack of cytotoxicity in the relevant cell cultures. In vivo intervention in a rat subacute MI model resulted in the full integration of the scaffold into the heart after implantation and biocompatibility with the tissue, with a prevalence of anti-inflammatory and pro-angiogenic macrophages, as well as evidence of revascularization and improved cardiac function after 60 days. Moreover, the beneficial effect of the released SDF1 on heart remodeling was confirmed by a significant reduction in cardiac tissue stiffness. Our findings demonstrate that this multimodal scaffold is a desirable matrix that can be used as a drug delivery system and a scaffolding material to promote functional recovery after MI

Non-covalently coated biopolymeric nanoparticles for improved tamoxifen delivery

About one-fifth of cancer patients suffer from breast cancer worldwide. Polymeric nanoparticles play an important role in delivering chemotherapeutic agents in a controlled manner. Polylysine coated tamoxifen loaded poly(lactic-co-glycolic acid) nanoparticles were prepared using a single emulsion technique with subsequent non-covalently surface functionalization in order to improve nanoparticle-cell interaction and hence tamoxifen therapeutic effect. The obtained nanoparticles were fully characterized in terms of their physico-chemical properties as well of their in vitro performance against human breast adenocarcinoma cells. The successful incorporation of tamoxifen within the hydrophobic matrix of nanoparticles is evidenced by a high loading efficiency (86%). Furthermore, ideal size, morphology and hydrodynamic properties are observed being the proposed nanocarrier capable of display a valuable antiproliferative in vitro effect.Fil: Chevalier, Merari Tumin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Rescignano, Nicoletta. Instituto en Ciencia y Tecnología de Polímeros; EspañaFil: Martin Saldaña, Sergio. Instituto en Ciencia y Tecnología de Polímeros; EspañaFil: González Gómez, Álvaro. Instituto en Ciencia y Tecnología de Polímeros; EspañaFil: Kenny, José Maria. Università di Perugia; ItaliaFil: San Román, Julio. Instituto en Ciencia y Tecnología de Polímeros; EspañaFil: Mijangos, Carmen. Instituto en Ciencia y Tecnología de Polímeros; EspañaFil: Alvarez, Vera Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentin

A multimodal scaffold for SDF1 delivery improves cardiac function in a rat subacute myocardial infarct model

Ischemic heart disease is one of the leading causes of death worldwide. The efficient delivery of therapeutic growth factors could counteract the adverse prognosis of post-myocardial infarction (post-MI). In this study, a collagen hydrogel that is able to load and appropriately deliver pro-angiogenic stromal cell-derived factor 1 (SDF1) was physically coupled with a compact collagen membrane in order to provide the suture strength required for surgical implantation. This bilayer collagen-on-collagen scaffold (bCS) showed the suitable physicochemical properties that are needed for efficient implantation, and the scaffold was able to deliver therapeutic growth factors after MI. In vitro collagen matrix biodegradation led to a sustained SDF1 release and a lack of cytotoxicity in the relevant cell cultures. In vivo intervention in a rat subacute MI model resulted in the full integration of the scaffold into the heart after implantation and biocompatibility with the tissue, with a prevalence of anti-inflammatory and pro-angiogenic macrophages, as well as evidence of revascularization and improved cardiac function after 60 days. Moreover, the beneficial effect of the released SDF1 on heart remodeling was confirmed by a significant reduction in cardiac tissue stiffness. Our findings demonstrate that this multimodal scaffold is a desirable matrix that can be used as a drug delivery system and a scaffolding material to promote functional recovery after MI