Sustainable Network by Enhancing Attribute-Based Selection Mechanism Using Lagrange Interpolation

The security framework in Ad-hoc Networks (ANET) continues to attract the attention of researchers, although significant work has been accomplished already. Researchers in the last couple of years have shown quite an improvement in Identity Dependent Cryptography (IDC). Security in ANET is hard to attain due to the vulnerability of links (Wireless). IDC encompasses Polynomial Interpolations (PI) such as Lagrange, curve-fitting, and spline to provide security by implementing Integrated Key Management (IKM). The PI structure trusts all the available nodes in the network and randomly picks nodes for the security key generation. This paper presents a solution to the trust issues raised in Lagrange’s-PI (LI) utilizing an artificial neural network and attribute-based tree structure. The proposed structure not only improves the trust factor but also enhances the accuracy measures of LI to provide a sustainable network system. Throughput, PDR, noise, and latency have been increased by 47%, 50%, 34%, and 30%, respectively, by using LI and incorporating the aforementioned techniques

Isolation and Taxonomic Characterization of Novel Haloarchaeal Isolates From Indian Solar Saltern: A Brief Review on Distribution of Bacteriorhodopsins and V-Type ATPases in Haloarchaea

Haloarchaea inhabit high salinity environments worldwide. They are a potentially rich source of crucial biomolecules like carotenoids and industrially useful proteins. However, diversity in haloarchaea present in Indian high salinity environments is poorly studied. In the present study, we isolated 12 haloarchaeal strains from hypersaline Kottakuppam, Tamil Nadu solar saltern in India. 16S rRNA based taxonomic characterization of these isolates suggested that nine of them are novel strains that belong to genera Haloarcula, Halomicrobium, and Haloferax. Transmission electron microscopy suggests the polymorphic nature of these haloarchaeal isolates. Most of the haloarchaeal species are known to be high producers of carotenoids. We were able to isolate carotenoids from all these 12 isolates. The UV-Vis spectroscopy-based analysis suggests that bacterioruberin and lycopene are the major carotenoids produced by these isolates. Based on the visual inspection of the purified carotenoids, the isolates were classified into two broad categories i.e., yellow and orange, attributed to the differences in the ratio of bacterioruberin and lycopene as confirmed by the UV-Vis spectral analysis. Using a PCR-based screening assay, we were able to detect the presence of the bacteriorhodopsin gene (bop) in 11 isolates. We performed whole-genome sequencing for three bop positive and one bop negative haloarchaeal isolates. Whole-genome sequencing, followed by pan-genome analysis identified multiple unique genes involved in various biological functions. We also successfully cloned, expressed, and purified functional recombinant bacteriorhodopsin (BR) from one of the isolates using Escherichia coli as an expression host. BR has light-driven proton pumping activity resulting in the proton gradient across the membrane, which is utilized by V-Type ATPases to produce ATP. We analyzed the distribution of bop and other accessory genes involved in functional BR expression and ATP synthesis in all the representative haloarchaeal species. Our bioinformatics-based analysis of all the sequenced members of genus Haloarcula suggests that bop, if present, is usually inserted between the genes coding for B and D subunits of the V-type ATPases operon. This study provides new insights into the genomic variations in haloarchaea and reports expression of new BR variant having good expression in functional form in E. coli

Société de rachat des marchandises, dettes et créances de l'industrie fraternelle . (Signé : Les commissaires, Bernard, etc.)

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Additional file 10: Figure S4. of Stringent comparative sequence analysis reveals SOX10 as a putative inhibitor of glial cell differentiation

SOX10 regulates genes that inhibit glial cell differentiation in vivo. Primary Schwann cells were extracted and grown from three independent rat adult sciatic nerves. Cells were treated with a control siRNA or an siRNA targeted against Sox10 as in Fig. 7c. Quantitative RT-PCR was used to measure expression levels of each indicated gene. The effect on expression of each gene (indicated across the bottom) is expressed relative to the control siRNA and error bars indicate standard deviations. Please note that, consistent with the in vitro data, Hmga2 did not show a decrease in expression levels in vivo (data not shown). (PDF 334 kb

Additional file 12: of Stringent comparative sequence analysis reveals SOX10 as a putative inhibitor of glial cell differentiation

DNase-seq peaks identified in S16 cells that are common to all three experimental replicates. The data are provided as a UCSC Rat Genome Browser (rn5) custom track and the fourth column (‘name’) shows the F-Seq score for the peak. (TXT 5171 kb

Smart Nanofibrous Hydrogel Wound Dressings for Dynamic Infection Diagnosis and Control: Soft but Functionally Rigid

Chronic wounds, such as burns and diabetic foot ulcers, pose significant challenges to global healthcare systems due to prolonged hospitalization and increased costs attributed to susceptibility to bacterial infections. The conventional use of antibiotic-loaded and metal-impregnated dressings exacerbates concerns related to multidrug resistance and skin argyrosis. In response to these challenges, our research introduces a unique approach utilizing antibiotic-free smart hydrogel wound dressings with integrated infection eradication and diagnostic capabilities. Electrospinning stands out as a method capable of producing hydrogel nanofibrous materials possessing favorable characteristics for treating wounds and detecting infections under conditions utilizing sustainable materials. In this study, innovative dressings are fabricated through electrospinning polycaprolactone (PCL)/gelatin (GEL) hybrid hydrogel nanofibers, incorporating pDA as a cross-linker, εPL as a broad-spectrum antimicrobial agent, and anthocyanin as a pH-responsive probe. The developed dressings demonstrate exceptional antioxidant (>90% radical scavenging) and antimicrobial properties (95–100% killing). The inclusion of polyphenols/flavonoids and εPL leads to absolute bacterial eradication, and in vitro assessments using HaCaT cells indicate increased cell proliferation, decreased reactive oxygen species (ROS) production, and enhanced cell viability (100% Cell viability). The dressings display notable alterations in color that correspond to different wound conditions. Specifically, they exhibit a red/violet hue under healthy wound conditions (pH 4–6.5) and a green/blue color under unhealthy wound conditions (pH > 6.5). These distinctive color changes provide valuable insights into the versatile applications of the dressings in the care and management of wounds. Our findings suggest that these antibiotic-free smart hydrogel wound dressings hold promise as an effective and sustainable solution for chronic wounds, providing simultaneous infection control and diagnostic monitoring. This research contributes to advancing the field of wound care, offering a potential paradigm shift in the development of next-generation wound dressings