On-Demand Urine Analyzer

A lab-on-a-chip was developed that is capable of extracting biochemical indicators from urine samples and generating their surface-enhanced Raman spectra (SERS) so that the indicators can be quantified and identified. The development was motivated by the need to monitor and assess the effects of extended weightlessness, which include space motion sickness and loss of bone and muscle mass. The results may lead to developments of effective exercise programs and drug regimes that would maintain astronaut health. The analyzer containing the lab-on-a- chip includes materials to extract 3- methylhistidine (a muscle-loss indicator) and Risedronate (a bone-loss indicator) from the urine sample and detect them at the required concentrations using a Raman analyzer. The lab-on- a-chip has both an extractive material and a SERS-active material. The analyzer could be used to monitor the onset of diseases, such as osteoporosis

Intra-spinal epidural leakage of bone cement during vertebroplasty of an osteoporotic vertebral fracture: case report and review of literature

Vertebral fractures are one of the most common complications of osteoporosis. Prolonged and intractable pain leads to immobilization and significant morbidity. Vertebroplasty is designed primarily to relieve pain, and the procedure is considered when osteoporotic vertebral fracture does not respond to a reasonable period of conservative care. Vertebroplasty has a low complication rate with most common complication being adjacent vertebral body fracture and rare complication due to extra-vertebral cement leakage causing nerve root compression or pulmonary embolism. We report a case of 55 year old lady with osteoporotic D12 wedge compression fracture subjected to vertebroplasty resulting in intraspinal cement leakage noticed intra-operatively. Patient underwent immediate decompression, cement extraction and posterior instrumentation. Postoperative course was uneventful

How long does treated supracondylar humerus fracture in children take to recover elbow range?

Background: Our goal was to address requirement of physiotherapy after treatment of supracondylar humerus fractures in children and evaluate result of casting on elbow without injury using control group of distal forearm fractures.Methods: 57 cases of supracondylar fractures were included in this retrospective study and compared with group of 54 children with distal forearm fractures treated with long arm casting for 28 days. Passive elbow ROM was checked with goniometer on day of cast removal, 2 weeks later, and then every month until the elbow ROM returned to 95% ROM (ROM-95) of uninjured side. No physiotherapy was given.Results: Average time in cast for supracondylar fracture group was 31.8 days and distal forearm fracture group was 32.4 days. The elbow total flexion angle reached a plateau of 139 degrees at 1month after cast removal whereas forearm group required less than 2 weeks. Total rotation ROM required half the time to reach 139 degrees in forearm group. From the paired t test it took more time for pronation to recover than supination in supracondylar fractures. In supracondylar group there was positive correlation between casting period and recovery period of ROM-95 elbow extension (p=0.021) and days of casting (p=0.021) and ROM-95 flexion recovery period. There was no statistically significant impact between casting and recovery period in other directions and recovery of ROM-95 & F-ROM and the subclassification and type of treatment of individual fractures.Conclusions: Lessons learned: thorough assessment of elbow function post cast removal, lack of need of physiotherapy in management protocol and elbow stiffness is related to initial injury and associated treatment not merely immobilisation

Multi-layer Tablet: Current scenario and recent advances

The purpose of this article on tableting of multiple Active Pharmaceutical Ingredients (APIs) in the single oral solid dose in the form of Fixed Dose Combinations (FDCs), that focused on therapeutic justification, designing, its practical approach, summarize the problem encountered and parameters to be considered during development. This will help budding formulation development scientists working on generic development as such types of formulation is current need in various disease conditions. Looking to the necessity of medical practitioners, ultimately generic pharmaceutical companies taking interest in development of such formulation with correct medical justification due to various health problem not only in India but also globally. FDCs are highly popular in the Indian pharmaceutical market and also have been seen in the last few years. Presently very few generic companies are working on FDCs in the form of multilayer tablets resulting into small numbers of formulation in the market yearly

High efficiency sol-gel gas chromatography column

A capillary column (10) includes a tube structure having inner walls (14) and a sol-gel substrate (16) coated on a portion of inner walls (14) to form a stationary phase coating (18) on inner walls (14). The sol solution used to prepare the sol-gel substrate (16) has at least one baseline stabilizing reagent and at least one surface deactivation reagent resulting in the sol-gel substrate (16) having at least one baseline stabilizing reagent residual and at least one surface deactivating reagent residual. A method of making the sol-gel solution is by mixing suitable sol-gel precursors to form the solution, stabilizing the solution by adding at least one baseline stabilization reagent, deactivating the solution by adding at least one surface deactivation reagent to the solution, and reacting the solution in the presence of at least one catalyst

Drug Stability Analysis by Raman Spectroscopy

Pharmaceutical drugs are available to astronauts to help them overcome the deleterious effects of weightlessness, sickness and injuries. Unfortunately, recent studies have shown that some of the drugs currently used may degrade more rapidly in space, losing their potency before their expiration dates. To complicate matters, the degradation products of some drugs can be toxic. Here, we present a preliminary investigation of the ability of Raman spectroscopy to quantify mixtures of four drugs; acetaminophen, azithromycin, epinephrine, and lidocaine, with their primary degradation products. The Raman spectra for the mixtures were replicated by adding the pure spectra of the drug and its degradant to determine the relative percent contributions using classical least squares. This multivariate approach allowed determining concentrations in ~10 min with a limit of detection of ~4% of the degradant. These results suggest that a Raman analyzer could be used to assess drug potency, nondestructively, at the time of use to ensure crewmember safety

Fish-farming in South Africa : a study of the market environment and the suitable species

Thesis (MPhil)--Stellenbosch University, 2015.ENGLISH ABSTRACT: Emerging global trends towards sustainable development have placed emphasis on the need to create sustainable food value chains. Many food value chains are becoming weakened through their reliance on eco-systems that are deteriorating. Furthermore, while food systems are under threat, global food consumption is on the rise. The conundrum seems inescapable. Humanity’s food supply methods have moved on from hunter-gatherer characteristics apart from the exception of the fishing industry. The current generation may be the last to witness the wide-spread practice of fishing. Aquaculture, and in particular fish-farming, has begun to show much potential as a method of producing sustainable sources of protein. Whether this can be utilized in the South African context is a challenge that needs to be verified. This research, presented as two articles, explores the potential for the development of the fish-farming sector in South Africa, and recommends suitable species. Although there is some literature on how fish-farming may have a place in South Africa’s sustainability quest, it is scarce. This research aims to enhance the literature base on fish-farming in South Africa, as well as provide further evidence on what the true potential is for fish-farming in South Africa. This was done through a series of primary and secondary data collection methods that allow for a thorough analysis of fish-farming in South Africa and the species involved. The analysis revealed that the fish-farming sector and the cultivatable species in South Africa are met with an array of opportunities and challenges. Overcoming these challenges will open the doors to exploit the opportunities available.AFRIKAANS OPSOMMING: Opkomende globale tendense weerspieël dat dit noodsaaklik is dat klem gelê moet word op volhoubare ontwikkeling en dat dit van uitterste belang is om volhoubare voedsel waardekettings te skep. Verskeie voedsel waardekettings raak verswak deur hul afhanklikheid van eko-stelsels wat vernietig word. Verder, terwyl voedsel stelsels bedreig word, is globale verbruik van voedsel besig om te verhoog. Dié raaisel lyk onvermydelik. Mensdom se huidige voedselvoorraad metodes het aansienlik ontwikkel van dié van die jagter-versamelaars, met die uitsondering van die visbedryf. Die huidige generasie mag die laaste wees, wat die wyd verspreide praktyk van visvang beoefen, om daarvan te getuig. Akwakultuur, in besonder vis-boerdery, het begin om potensiaal te wys as 'n volhoubare bron in die vervaardiging van proteïen. Of dit in die Suid-Afrikaanse konteks gebruik kan word, is 'n uitdaging wat geverifieer moet word. Hierdie navorsing, wat aangebied word as twee tydskrifartikels, ondersoek die potensiaal vir die ontwikkeling van vis-boerdery in Suid-Afrika, en beveel ook gepaste spesies aan. Alhoewel daar sommige artikels is oor hoe vis-boerdery kan in pas in Suid-Afrika se soeke na volhoubaarheid, is dit skaars te vinde. Die navorsing beoog om die literatuur basis van vis-boerdery in Suid-Afrika op te skerp, en ter selfde tyd verdere bewys te gee oor die ware potensiaal van vis-boerdery in Suid-Afrika. Dit was gedoen deur 'n reeks van primêre en sekondêre data-insameling metodes wat voorsiening maak vir 'n deeglike ontleding van visboerdery in Suid -Afrika en die spesies betrokke. Die ontleding het getoon dat die vis-boerdery bedryf en die aankweek spesies in Suid-Afrika verskeie geleenthede en uitdagings in die gesig staar. Oorwinning van hierdie uitdagings sal dit moontlik maak om die beskikbare geleenthede te ontgin

Quantitative Measurements of Codeine and Fentanyl on a Surface-Enhanced Raman-Active Pad

The USA is in the midst of an opioid crisis that included over 60,000 overdose fatalities in 2017, mostly unintentional. This is due to excessive use of prescription opioids and the use of very strong synthetic opioids, such as fentanyl, mixed with illicit street drugs. The ability to rapidly determine if people or packages entering the country have or contain drugs could reduce their availability, and thereby decrease the use of illicit drugs. In an effort to address this problem, we have been investigating the ability of surface-enhanced Raman spectroscopy to detect trace amounts of opioids on clothing and packages. Here, we report the measurement of codeine and fentanyl at 100 ng/mL for 5 min on a pad impregnated with gold colloids, as well as a preliminary measurement of 500 pg of fentanyl on a glass surface using one of these pads. The calculated limit of detection for this measurement was 40 pg. This data strongly suggests that these pads, used with portable Raman analyzers, would be invaluable to airport security, drug raids, crime scenes, and forensic analysis