Nutritional Value of Fermented Maize Stover as Feed for Ruminant

An experiment was undertaken to determine the physical and nutritional properties, in Vitro digestibility of maize stover fermented with different levels of molasses. Maize stover was collected from the field immediately after harvesting the corn, chopped and were preserved in plastic containers under normal condition and were treated, sample fermented without additives (T1), a sample with the addition of water and 5% molasses (T2). After completion of each ensiled period, nutritional properties, in-vitro digestibility and were determined. The result reveals that crude protein (CP), dry matter (DM), ash content of maize stover were increased (P<0.05) while the crude fiber (CF) contents were decreased (P<0.01) after ensiling and the addition of molasses. The highest DM, EE, and CP content was found to be 99.52%, 10.33% and 4.56% in T2 while CF was found highest in T1 32.79%. The OMD(organic matter digestibility) and DMD (dry matter digestibility) contents were decreased by ensiling with molasses. The highest DMD, OMD, N-NH3, total VFA DMD values were observed in treatmentT1 which were 47.71%, 46.78%, 12.50%, 5.42% respectively. Therefore, it can be concluded that the addition of molasses improved and nutritional properties and preservation capacity of maize stover after 21 days of ensilingKeywords: Fermented, Maize stover, Molasses, Nutritional properties, Ruminan

The Effect of Multistage Ammoniation on Fiber Fraction and Digestibility of Maize Stover in vitro

The main objective of this experiment was to assess the effect of multistage ammoniation on fiber fraction and digestibility of maize stover in vitro. Maize stover was treated by a multistage technique of different urea connect ration (8%, 4%, and 2%) and incubation for 14 days at room temperature.Digestibility of NDF, ADF, ADL, hemicellulose, and cellulose, in vitro dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N, total volatile fatty acid (VFA) and pH concentration were observed. However treatment maize stover and multistage ammoniation maize stover were composed by using T-test student as the statistic. There were not significantly different on fiber fraction and digestibility of maize stover. However, multistage ammoniation decreased ADF fraction by 56.67% to45.39%. The DM digestibility of multistage ammoniation on fiber fraction and digestibility of maize stover also increased by 35.18 to 45.91. The total VFA and N-ammonia of multistage ammoniation on fiber fraction and digestibility of maize stover also higher than control.Keywords: Ammoniation, Digestibility, in vitro, Maize stove

Changes of Microbial Population in the Rumen of Dairy Steers as Influenced by Plant Containing Tannins and Saponins and Roughage to Concentrate Ratio

The objective of this study was to investigate microbial population in the rumen of dairy steers as influenced by supplementing with dietary condensed tannins and saponins and different roughage to concentrate ratios. Four, rumen fistulated dairy steers (Bos indicus) were used in a 2×2 factorial arrangement in a 4×4 Latin square design. The main factors were two roughage to concentrate ratios (R:C, 60:40 and 40:60) and two supplementations of rain tree pod meal (RPM) (0 and 60 g/kg of total DM intake). Chopped 30 g/kg urea treated rice straw was used as a roughage source. All animals received feed according to respective R:C ratios at 25 g/kg body weight. The RPM contained crude tannins and saponins at 84 and 143 g/kg of DM, respectively. It was found that ruminal pH decreased while ruminal temperature increased by a higher concentrate ratio (R:C 40:60) (p<0.05). In contrast, total bacterial, Ruminococus albus and viable proteolytic bacteria were not affected by dietary supplementation. Numbers of fungi, cellulolytic bacteria, Fibrobactor succinogenes and Ruminococus flavefaciens were higher while amylolytic bacteria was lower when steers were fed at 400 g/kg of concentrate. The population of Fibrobactor succinogenes, was found to be higher with RPM supplementation. In addition, the use of real-time PCR technique indicated that the population of protozoa and methanogens were decreased (p<0.05) with supplementation of RPM and with an increasing concentrate ratio. Supplementation of RPM and feeding different concentrate ratios resulted in changing the rumen microbes especially, when the animals were fed at 600 g/kg of concentrate and supplemented with RPM which significantly reduced the protozoa and methanogens population

Effect of Plants Containing Secondary Compounds with Palm Oil on Feed Intake, Digestibility, Microbial Protein Synthesis and Microbial Population in Dairy Cows

The objective of this study was to determine the effect of rain tree pod meal with palm oil supplementation on feed intake, digestibility, microbial protein synthesis and microbial populations in dairy cows. Four, multiparous early-lactation Holstein-Friesian crossbred (75%) lactating dairy cows with an initial body weight (BW) of 405±40 kg and 36±8 DIM were randomly assigned to receive dietary treatments according to a 4×4 Latin square design. The four dietary treatments were un-supplementation (control), supplementation with rain tree pod meal (RPM) at 60 g/kg, supplementation with palm oil (PO) at 20 g/kg, and supplementation with RPM at 60 g/kg and PO at 20 g/kg (RPO), of total dry matter intake. The cows were offered concentrates, at a ratio of concentrate to milk production of 1:2, and chopped 30 g/kg of urea treated rice straw was fed ad libitum. The RPM contained condensed tannins and crude saponins at 88 and 141 g/kg of DM, respectively. It was found that supplementation with RPM and/or PO to dairy cows diets did not show negative effects on feed intake and ruminal pH and BUN at any times of sampling (p>0.05). However, RPM supplementation resulted in lower crude protein digestibility, NH3-N concentration and number of proteolytic bacteria. It resulted in greater allantoin absorption and microbial crude protein (p<0.05). In addition, dairy cows showed a higher efficiency of microbial N supply (EMNS) in both RPM and RPO treatments. Moreover, NDF digestibility and cellulolytic bacteria numbers were highest in RPO supplementation (p<0.05) while, supplementation with RPM and/or PO decreased the protozoa population in dairy cows. Based on this study, supplementation with RPM and/or PO in diets could improve fiber digestibility, microbial protein synthesis in terms of quantity and efficiency and microbial populations in dairy cows

Optimal Cultivation Time for Yeast and Lactic Acid Bacteria in Fermented Milk and Effects of Fermented Soybean Meal on Rumen Degradability Using Nylon Bag Technique

The objectives of this study were to determine an optimal cultivation time for populations of yeast and lactic acid bacteria (LAB) co-cultured in fermented milk and effects of soybean meal fermented milk (SBMFM) supplementation on rumen degradability in beef cattle using nylon bag technique. The study on an optimal cultivation time for yeast and LAB growth in fermented milk was determined at 0, 4, 8, 24, 48, 72, and 96 h post-cultivation. After fermenting for 4 days, an optimal cultivation time of yeast and LAB in fermented milk was selected and used for making the SBMFM product to study nylon bag technique. Two ruminal fistulated beef cattle (410±10 kg) were used to study on the effect of SBMFM supplementation (0%, 3%, and 5% of total concentrate substrate) on rumen degradability using in situ method at incubation times of 0, 2, 4, 6, 12, 24, 48, and 72 h according to a Completely randomized design. The results revealed that the highest yeast and LAB population culture in fermented milk was found at 72 h-post cultivation. From in situ study, the soluble fractions at time zero (a), potential degradability (a+b) and effective degradability of dry matter (EDDM) linearly (p<0.01) increased with the increasing supplemental levels and the highest was in the 5% SBMFM supplemented group. However, there was no effect of SBMFM supplement on insoluble degradability fractions (b) and rate of degradation (c). In conclusion, the optimal fermented time for fermented milk with yeast and LAB was at 72 h-post cultivation and supplementation of SBMFM at 5% of total concentrate substrate could improve rumen degradability of beef cattle. However, further research on effect of SBMFM on rumen ecology and production performance in meat and milk should be conducted using in vivo both digestion and feeding trials

Effect of Carbohydrate Source and Cottonseed Meal Level in the Concentrate on Feed Intake, Nutrient Digestibility, Rumen Fermentation and Microbial Protein Synthesis in Swamp Buffaloes

The objective of this study was to investigate the effect of carbohydrate source and cottonseed meal level in the concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in swamp buffaloes. Four, 4-yr old rumen fistulated swamp buffaloes were randomly assigned to receive four dietary treatments according to a 2×2 factorial arrangement in a 4×4 Latin square design. Factor A was carbohydrate source; cassava chip (CC) and CC+rice bran at a ratio 3:1 (CR3:1), and factor B was level of cottonseed meal (CM); 109 g CP/kg (LCM) and 328 g CP/kg (HCM) in isonitrogenous diets (490 g CP/kg). Buffaloes received urea-treated rice straw ad libitum and supplemented with 5 g concentrate/kg BW. It was found that carbohydrate source did not affect feed intake, nutrient intake, digested nutrients, nutrient digestibility, ammonia nitrogen concentration, fungi and bacterial populations, or microbial protein synthesis (p>0.05). Ruminal pH at 6 h after feeding and the population of protozoa at 4 h after feeding were higher when buffalo were fed with CC than in the CR3:1 treatment (p0.05). Based on this experiment, concentrate with a low level of cottonseed meal could be fed with cassava chips as an energy source in swamp buffalo receiving rice straw

Rumen characteristics and feed utilization in goats fed with biologically treated oil palm fronds as roughage in a total mixed ration

Six male crossbred (Thai Native x Anglo Nubian) goats, 33.5 ± 1.7 kg body weight (BW), were randomly assigned to a 3 x 3 replicated Latin square design to investigate the effects of fungal-treated oil palm fronds (FTOPF) on feed intake, digestibility, ruminal fermentation and nitrogen utilization. The treatments were untreated oil palm frond (UOPF; T1), fungal treated oil palm frond (FTOPF; T2) and fungal treated oil palm frond with urea 1% (FTOPFU; T3). The results revealed that voluntary feed intake was not significantly different among treatments, while digestion coefficients of nutrients (DM, OM, CP, NDF, and ADF) increased significantly with FTOPF. However, the mean ruminal temperature, pH, NH3-N, blood urea-nitrogen and amount of N absorbed and retained were similar among treatments. In conclusion, FTOPF could be included in total mixed rations without any adverse effect on feed intake, digestibility and rumen fermentation efficiency, suggesting that FTOPF could be used potential as an alternative roughage source for ruminants.Keywords: Agricultural by-product, digestibility, fungal treatment, Lentinussajor-caju, small ruminan

Gas Kinetics, Rumen Characteristics, and In Vitro Degradability of Varied Levels of Dried and Fresh Cassava Leaf Top Fermented with Cassava Pulp

The purpose of this study was to determine the impact of different levels of dried cassava leaf top (DCT) and fresh cassava leaf top (FCT) fermented with cassava pulp (CS) on the nutritional value of silage, gas kinetics, rumen characteristics, and in vitro degradability. Dietary treatments were administered using a completely randomized design (CRD) with eight treatments and three replicate runs. The eight treatments were as follows: 1) CS fermented no additive (nA), 2) CS fermented with additives (Saccharomyces cerevisiae, urea, molasses, and sugar) (CSA), 3) 95% CSA fermented with 5% DCT (5DCT), 4) 90% CSA fermented with 10% DCT (10DCT), 5) 85% CSA fermented with 15% DCT (15DCT), 6) 95% CSA fermented with 5% FCT (5FCT), 7) 90% CSA fermented with 10% FCT (10FCT), 8) 85% CSA fermented with 15% FCT (15FCT), respectively. After 21 days of fermentation, samples of the silages were taken for chemical analysis and utilized to examine the in vitro gas production and degradability. The results show that fermented CS with DCT at 5% to 10% DM had the highest increase in CP when compared to nA or CSA (p&lt;0.05). In vitro dry matter disappearance (IVDMD) was significantly higher in CS fermented with 5% to 10% DCT (p&lt;0.01), whereas CS fermented with FCT levels demonstrated lower IVDMD than the control group (p&lt;0.01). The gas potential extent of gas production (p) and gas production from the insoluble fraction (b) did not differ significantly across treatments (p&gt;0.05). However,&nbsp;the gas production from the immediately soluble fraction (a)&nbsp;was maximum when CS was fermented with 15DCT (p&lt;0.05). Different treatments significantly affected the pH of the fermentation solution with the addition of 10DCT and 15DCT for 12 and 24 hours of incubation, respectively (p&lt;0.01). After 12 hours of incubation, the population of protozoa was lowest when 5DCT and 10DCT were evaluated (p&lt;0.01). In conclusion, CS fermented with DCT at a concentration of 5% to 10% can increase crude protein content, in vitro dry matter disappearance (IVDMD), and gas production from the immediately soluble fraction while decreasing the protozoa population