MiR 26 targeting methyltetrahydrofolate reductase inhibits the proliferation of acute myeloid leukemia cells via JAK/STAT pathway

Purpose: To investigate the effect of microribonucleic acid (miR)-26 targeting methyltetrahydrofolate reductase (MTHFR) on cell proliferation, cycle, and apoptosis in acute myeloid leukemia (AML).Methods: The expressions of miR-26 in three types of human AML cell lines (HL-60, Kasumi-1, and KG-1a) and normal myeloid cell line (HS-5) were determined via quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR), while the effect of the over-expression of miR-26 on the proliferation, cell cycle and apoptosis of AML cells was evaluated using cell counting kit-8 (CCK-8) assay and flow cytometry. The potential target for miR-26 was predicted using public miRNA database TargetScan, and whether miR-26 binds to the predicted target was determined using a dual-luciferase reporter assay. Western blotting was performed to determine the effect of miR-26 on Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway-associated proteins.Results: Expression level of miR-26 was significantly lower in all AML cell lines than in HS-5 cells (p < 0.05). Overexpression of miR-26 inhibited the proliferation of KG-1a cells, reduced the percentage of cells in S phase, increased those in G0/G1 phase, and enhanced apoptosis of KG-1a cells (p < 0.05). After overexpression of miR-26, protein expression levels of phosphorylated (p)-JAK and p-STAT were down-regulated, while those of JAK and STAT did not change significantly.Conclusion: Expression of miR-26 is down-regulated in AML, while MiR-26 targeting of MTHFR induces apoptosis and cycle arrest of AML cells through the JAK/STAT pathway, thus inhibiting AML cell proliferation in vitro

Intrusion of polyethylene glycol into solid-state nanopores

The intrusion of PEG aqueous solution into solid-state-nanopores upon mechanical pressure is experimentally investigated. By using hydrophobic nanoporous silica with a broad range of pore sizes, the characteristic size of PEG chains in water while penetrating nanopores is measured and analyzed, which increases with molecular weight and decreases with concentration of PEG. Its sensitivity to molecular weight is relatively limited due to nano-confinement. The inclusion of PEG as an intruding liquid imposes a rate effect on the intrusion pressure, and inhibits the extrusion from the nanopores

The Correspondence between Convergence Peaks from Weak Lensing and Massive Dark Matter Haloes

The convergence peaks, constructed from galaxy shape measurement in weak lensing, is a powerful probe of cosmology as the peaks can be connected with the underlined dark matter haloes. However the capability of convergence peak statistic is affected by the noise in galaxy shape measurement, signal to noise ratio as well as the contribution from the projected mass distribution from the large-scale structures along the line of sight (LOS). In this paper we use the ray-tracing simulation on a curved sky to investigate the correspondence between the convergence peak and the dark matter haloes at the LOS. We find that, in case of no noise and for source galaxies at $z_{\rm s}=1$, more than $65\%$ peaks with $\text{SNR} \geq 3$ (signal to noise ratio) are related to more than one massive haloes with mass larger than $10^{13} {\rm M}_{\odot}$. Those massive haloes contribute $87.2\%$ to high peaks ($\text{SNR} \geq 5$) with the remaining contributions are from the large-scale structures. On the other hand, the peaks distribution is skewed by the noise in galaxy shape measurement, especially for lower SNR peaks. In the noisy field where the shape noise is modelled as a Gaussian distribution, about $60\%$ high peaks ($\text{SNR} \geq 5$) are true peaks and the fraction decreases to $20\%$ for lower peaks ($3 \leq \text{SNR} < 5$). Furthermore, we find that high peaks ($\text{SNR} \geq 5$) are dominated by very massive haloes larger than $10^{14} {\rm M}_{\odot}$.Comment: 13 pages, 11 figures, 4 tables, accepted for publication in MNRAS. Our mock galaxy catalog is available upon request by email to the author ([email protected]

Wet Chemical Controllable Synthesis of Hematite Ellipsoids with Structurally Enhanced Visible Light Property

A facile and economic route has been presented for mass production of micro/nanostructured hematite microcrystals based on the wet chemical controllable method. The as-prepared samples were characterized using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and UV-Vis absorption spectroscopy. The results showed that the product was mesoporous α-Fe2O3 and nearly elliptical in shape. Each hematite ellipsoid was packed by many α-Fe2O3 nanoparticles. The values of vapor pressure in reaction systems played vital roles in the formation of porous hematite ellipsoids. Optical tests demonstrated that the micro/nanostructured elliptical hematite exhibited enhanced visible light property at room temperature. The formation of these porous hematite ellipsoids could be attributed to the vapor pressure induced oriented assembling of lots of α-Fe2O3 nanoparticles

Rate effect of liquid infiltration into mesoporous materials

Rate effect of liquid infiltration in mesopores is associated with both liquid viscosity and the solid–liquid interfacial effect.</p

Practical and Secure Outsourcing Algorithms of Matrix Operations Based on a Novel Matrix Encryption Method

With the recent growth and commercialization of cloud computing, outsourcing computation has become one of the most important cloud services, which allows the resource-constrained clients to efficiently perform large-scale computation in a pay-per-use manner. Meanwhile, outsourcing large scale computing problems and computationally intensive applications to the cloud has become prevalent in the science and engineering computing community. As important fundamental operations, large-scale matrix multiplication computation (MMC), matrix inversion computation (MIC), and matrix determinant computation (MDC) have been frequently used. In this paper, we present three new algorithms to enable secure, verifiable, and efficient outsourcing of MMC, MIC, and MDC operations to a cloud that may be potentially malicious. The main idea behind our algorithms is a novel matrix encryption/decryption method utilizing consecutive and sparse unimodular matrix transformations. Compared to previous works, this versatile technique can be applied to many matrix operations while achieving a good balance between security and efficiency. First, the proposed algorithms provide robust confidentiality by concealing the local information of the entries in the input matrices. Besides, they also protect the statistic information of the original matrix. Moreover, these algorithms are highly efficient. Our theoretical analysis indicates that the proposed algorithms reduce the time overhead on the client side from O(n 2.3728639 ) to O(n 2 ). Finally, the extensive experimental evaluations demonstrate the practical efficiency and effectiveness of our algorithms

Cell-Free Seminal mRNA and MicroRNA Exist in Different Forms

BACKGROUND: The great interest in cell-free mRNA, microRNA (miRNA) as molecular biomarkers for clinical applications, and as 'signaling' molecules for intercellular communication highlights the need to reveal their physical nature. Here this issue was explored in human cell-free seminal mRNA (cfs-mRNA) and miRNA (cfs-miRNA). METHODOLOGY/PRINCIPAL FINDINGS: Selected male reproductive organ-specific mRNAs, miRNAs, and piRNAs were quantified by quantitative real-time PCR in all experiments. While the stability of cfs-miRNA assessed by time-course analysis (up to 24 h at room temperature) was similar with cfs-mRNA, the reductive changes between cfs-miRNA and cfs-mRNA after filtration and Triton X-100 treatment on seminal plasma were very different, implying their different physical nature. Seminal microvesicles (SMVs) were then recovered and proportions of cfs-mRNA and cfs-miRNA within SMVs were quantified. The amounts of SMVs- sequestered cfs-mRNAs almost were the same as total cfs-mRNA, and were highly variable depending on the different sizes of SMVs. But most of cfs-miRNA was independent of SMVs and existed in the supernatant. The possible form of cfs-miRNA in the supernatant was further explored by filtration and protease K digestion. It passed through the 0.10-µm pore, but was degraded dramatically after intense protease K digestion. CONCLUSIONS/SIGNIFICANCE: The predominant cfs-mRNA is contained in SMVs, while most cfs-miRNA is bound with protein complexes. Our data explained the stability of extracellular RNAs in human semen, and shed light on their origins and potential functions in male reproduction, and strategy of developing them as biomarkers of male reproductive system

Elastomeric cellular structure enhanced by compressible liquid filler

Elastomeric cellular structures provide a promising solution for energy absorption. Their flexible and resilient nature is particularly relevant to protection of human bodies. Herein we develop an elastomeric cellular structure filled with nanoporous material functionalized (NMF) liquid. Due to the nanoscale infiltration in NMF liquid and its interaction with cell walls, the cellular structure has a much enhanced mechanical performance, in terms of loading capacity and energy absorption density. Moreover, it is validated that the structure is highly compressible and self-restoring. Its hyper-viscoelastic characteristics are elucidated