1,177 research outputs found
High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis
<p>Abstract</p> <p>Background</p> <p>Combination of <it>CHD </it>(chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-intensive and gel-required. Gender determination often fails when the difference in length between the PCR products of <it>CHD-Z </it>and <it>CHD-W </it>genes is too short to be resolved.</p> <p>Results</p> <p>Here, we are the first to introduce a PCR-melting curve analysis (PCR/MCA) to identify the gender of birds by genomic DNA, which is gel-free, quick, and inexpensive. <it>Spilornis cheela hoya </it>(<it>S. c. hoya</it>) and <it>Pycnonotus sinensis </it>(<it>P. sinensis</it>) were used to illustrate this novel molecular sexing technique. The difference in the length of <it>CHD </it>genes in <it>S. c. hoya </it>and <it>P. sinensis </it>is 13-, and 52-bp, respectively. Using Griffiths' P2/P8 primers, molecular sexing failed both in PCR/electrophoresis of <it>S. c. hoya </it>and in PCR/MCA of <it>S. c. hoya </it>and <it>P. sinensis</it>. In contrast, we redesigned sex-specific primers to yield 185- and 112-bp PCR products for the <it>CHD-Z </it>and <it>CHD-W </it>genes of <it>S. c. hoya</it>, respectively, using PCR/MCA. Using this specific primer set, at least 13 samples of <it>S. c. hoya </it>were examined simultaneously and the Tm peaks of <it>CHD-Z </it>and <it>CHD-W </it>PCR products were distinguished.</p> <p>Conclusion</p> <p>In this study, we introduced a high-throughput avian molecular sexing technique and successfully applied it to two species. This new method holds a great potential for use in high throughput sexing of other avian species, as well.</p
The Role of the Fabrication of Anatase-TiO2 Chain-Networked Photoanodes
A novel, sandwich-layered Ti/TiO2/Ti/fluorine-doped tin oxide (FTO) architecture is reported. The Ti layers play a critical role in the formation of long pear-necklace chains made of interconnected TiO2 nanoparticles. The chains interpenetrate a network structure on FTO glass substrates under alkaline hydrothermal-processing conditions. A significant enhancement in the photocurrent density of dye-sensitized solar cells employing non-volatile polymer-based electrolytes is obtained
Weak and Strong Lensing Statistics
After a brief introduction to gravitational lensing theory, a rough overview
of the types of gravitational lensing statistics that have been performed so
far will be given. I shall then concentrate on recent results of galaxy-galaxy
lensing, which indicate that galactic halos extend much further than can be
probed via rotation of stars and gas.Comment: 10 pages, 2 figures, talk given at the ISSI-Workshop "Matter in the
Universe", 19-23 March 2001 Bern (Switzerland
On walls of marginal stability in N=2 string theories
We study the properties of walls of marginal stability for BPS decays in a
class of N=2 theories. These theories arise in N=2 string compactifications
obtained as freely acting orbifolds of N=4 theories, such theories include the
STU model and the FHSV model. The cross sections of these walls for a generic
decay in the axion-dilaton plane reduce to lines or circles. From the
continuity properties of walls of marginal stability we show that central
charges of BPS states do not vanish in the interior of the moduli space. Given
a charge vector of a BPS state corresponding to a large black hole in these
theories, we show that all walls of marginal stability intersect at the same
point in the lower half of the axion-dilaton plane. We isolate a class of
decays whose walls of marginal stability always lie in a region bounded by
walls formed by decays to small black holes. This enables us to isolate a
region in moduli space for which no decays occur within this class. We then
study entropy enigma decays for such models and show that for generic values of
the moduli, that is when moduli are of order one compared to the charges,
entropy enigma decays do not occur in these models.Comment: 40 pages, 2 figure
A switchable pH-differential unitized regenerative fuel cell with high performance
Regenerative fuel cells are a potential candidate for future energy storage, but their applications are limited by the high cost and poor round-trip efficiency. Here we present a switchable pH-differential unitized regenerative fuel cell capable of addressing both the obstacles. Relying on a membraneless laminar flow-based design, pH environments in the cell are optimized independently for different electrode reactions and are switchable together with the cell process to ensure always favorable thermodynamics for each electrode reaction. Benefiting from the thermodynamic advantages of the switchable pH-differential arrangement, the cell allows water electrolysis at a voltage of 0.57 V, and a fuel cell open circuit voltage of 1.89 V, rendering round-trip efficiencies up to 74%. Under room conditions, operating the cell in fuel cell mode yields a power density of 1.3 W cm¯², which is the highest performance to date for laminar flow-based cells and is comparable to state-of-the-art polymer electrolyte membrane fuel cells
Theory of coherent acoustic phonons in InGaN/GaN multi-quantum wells
A microscopic theory for the generation and propagation of coherent LA
phonons in pseudomorphically strained wurzite (0001) InGaN/GaN multi-quantum
well (MQW) p-i-n diodes is presented. The generation of coherent LA phonons is
driven by photoexcitation of electron-hole pairs by an ultrafast Gaussian pump
laser and is treated theoretically using the density matrix formalism. We use
realistic wurzite bandstructures taking valence-band mixing and strain-induced
piezo- electric fields into account. In addition, the many-body Coulomb
ineraction is treated in the screened time-dependent Hartree-Fock
approximation. We find that under typical experimental conditions, our
microscopic theory can be simplified and mapped onto a loaded string problem
which can be easily solved.Comment: 20 pages, 17 figure
Immersed boundary-finite element model of fluid-structure interaction in the aortic root
It has long been recognized that aortic root elasticity helps to ensure
efficient aortic valve closure, but our understanding of the functional
importance of the elasticity and geometry of the aortic root continues to
evolve as increasingly detailed in vivo imaging data become available. Herein,
we describe fluid-structure interaction models of the aortic root, including
the aortic valve leaflets, the sinuses of Valsalva, the aortic annulus, and the
sinotubular junction, that employ a version of Peskin's immersed boundary (IB)
method with a finite element (FE) description of the structural elasticity. We
develop both an idealized model of the root with three-fold symmetry of the
aortic sinuses and valve leaflets, and a more realistic model that accounts for
the differences in the sizes of the left, right, and noncoronary sinuses and
corresponding valve cusps. As in earlier work, we use fiber-based models of the
valve leaflets, but this study extends earlier IB models of the aortic root by
employing incompressible hyperelastic models of the mechanics of the sinuses
and ascending aorta using a constitutive law fit to experimental data from
human aortic root tissue. In vivo pressure loading is accounted for by a
backwards displacement method that determines the unloaded configurations of
the root models. Our models yield realistic cardiac output at physiological
pressures, with low transvalvular pressure differences during forward flow,
minimal regurgitation during valve closure, and realistic pressure loads when
the valve is closed during diastole. Further, results from high-resolution
computations demonstrate that IB models of the aortic valve are able to produce
essentially grid-converged dynamics at practical grid spacings for the
high-Reynolds number flows of the aortic root
Simultaneous Diagonal and Off Diagonal Order in the Bose--Hubbard Hamiltonian
The Bose-Hubbard model exhibits a rich phase diagram consisting both of
insulating regimes where diagonal long range (solid) order dominates as well as
conducting regimes where off diagonal long range order (superfluidity) is
present. In this paper we describe the results of Quantum Monte Carlo
calculations of the phase diagram, both for the hard and soft core cases, with
a particular focus on the possibility of simultaneous superfluid and solid
order. We also discuss the appearance of phase separation in the model. The
simulations are compared with analytic calculations of the phase diagram and
spin wave dispersion.Comment: 28 pages plus 24 figures, uuencoded Revtex+postscript file
Mechanical properties of an Al-Zn-Mg alloy processed by ECAP and heat treatments
An investigation was conducted to study the influence of equal-channel angular pressing (ECAP) and post-ECAP aging at 393 K for 20 h on the microstructures and tensile properties of a supersaturated Al-Zn-Mg alloy together with the effect of pre-ECAP heat treatments on the mechanical properties of the alloy after ECAP and after post-ECAP heat treatments. The results show that during ECAP processing for up to 4 passes for the supersaturated Al alloy there is a simultaneous occurrence of grain refinement, increases in the dislocation density and dynamic aging precipitation forming large numbers of fine spherical well-distributed precipitates which enhance the yield strength but decrease the ductility. During post-ECAP aging, there is a limited dislocation recovery with slight grain growth and the precipitate sizes increase together with the formation of a few larger platelet precipitates and the transformation of G.P. zones to η′ and η′ to η leading to a strength reduction after 4 passes of ECAP. The precipitates in the ECAP-processed alloy with pre-ECAP in the supersaturated state formed through dynamic aging precipitation are higher in their volume fraction, smaller in their size and more homogeneously distributed in the Al matrix than those in the alloy with pre-ECAP in the peak aging state which mainly come from the fragmented η′ existing in the matrix before ECAP. The strengths of the alloy both after ECAP processing and after post-ECAP heat treatments with pre-ECAP in the supersaturation state are higher than with pre-ECAP in the peak aging state
Entanglement Transfer via XXZ Heisenberg chain with DM Interaction
The role of spin-orbit interaction, arises from the Dzyaloshinski-Moriya
anisotropic antisymmetric interaction, on the entanglement transfer via an
antiferromagnetic XXZ Heisenberg chain is investigated. From symmetrical point
of view, the XXZ Hamiltonian with Dzyaloshinski-Moriya interaction can be
replaced by a modified XXZ Hamiltonian which is defined by a new exchange
coupling constant and rotated Pauli operators. The modified coupling constant
and the angle of rotations are depend on the strength of Dzyaloshinski-Moriya
interaction. In this paper we study the dynamical behavior of the entanglement
propagation through a system which is consist of a pair of maximally entangled
spins coupled to one end of the chain. The calculations are performed for the
ground state and the thermal state of the chain, separately. In both cases the
presence of this anisotropic interaction make our channel more efficient, such
that the speed of transmission and the amount of the entanglement are improved
as this interaction is switched on. We show that for large values of the
strength of this interaction a large family of XXZ chains becomes efficient
quantum channels, for whole values of an isotropy parameter in the region .Comment: 21 pages, 9 figure
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