54 research outputs found

    Whole transcriptome analysis reveals non-coding RNA's competing endogenous gene pairs as novel form of motifs in serous ovarian cancer

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    Publisher Copyright: © 2022The non-coding RNA (ncRNA) regulation appears to be associated to the diagnosis and targeted therapy of complex diseases. Motifs of non-coding RNAs and genes in the competing endogenous RNA (ceRNA) network would probably contribute to the accurate prediction of serous ovarian carcinoma (SOC). We conducted a microarray study profiling the whole transcriptomes of eight human SOCs and eight controls and constructed a ceRNA network including mRNAs, long ncRNAs, and circular RNAs (circRNAs). Novel form of motifs (mRNA-ncRNA-mRNA) were identified from the ceRNA network and defined as non-coding RNA's competing endogenous gene pairs (ceGPs), using a proposed method denoised individualized pair analysis of gene expression (deiPAGE). 18 cricRNA's ceGPs (cceGPs) were identified from multiple cohorts and were fused as an indicator (SOC index) for SOC discrimination, which carried a high predictive capacity in independent cohorts. SOC index was negatively correlated with the CD8+/CD4+ ratio in tumour-infiltration, reflecting the migration and growth of tumour cells in ovarian cancer progression. Moreover, most of the RNAs in SOC index were experimentally validated involved in ovarian cancer development. Our results elucidate the discriminative capability of SOC index and suggest that the novel competing endogenous motifs play important roles in expression regulation and could be potential target for investigating ovarian cancer mechanism or its therapy.Peer reviewe

    Scour protection of submarine pipelines using rubber plates underneath the pipes

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    YesThis paper presents the results from laboratory experiments to investigate the protection of scour around submarine pipelines under unidirectional flow using a rubber plate placed underneath the pipes. The pressure difference on the two sides of the pipeline is the driving force to initiate the movement of sediment particles and can be obtained by force balance analysis. Experiments covering a wide range of incoming flow velocity, pipe diameter and plate length show that there exists a critical pressure difference over which the movement of sediment and, thus, scour takes place. Analysis of the experimental results demonstrates that this critical pressure difference is related to the pressure difference of the axial points between upstream and downstream of the pipe, which can be easily determined. This critical pressure difference is used to develop an empirical formula for estimating the critical length of the rubber plate, over which the sediment movement and scour will not take place. Good agreement between the experiments and calculated critical plate length using the proposed formula is obtained.National High-Tech Research and Development program of China (863 Program, Grant No.2008AA09Z309), National Nature Science Fund of China (Grant No.50879084, 51279071 and 51279189), the Open Funding from the State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University (SKLH-OF-1306

    Flow in Open Channel with Complex Solid Boundary

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    yesA two-dimensional steady potential flow theory is applied to calculate the flow in an open channel with complex solid boundaries. The boundary integral equations for the problem under investigation are first derived in an auxiliary plane by taking the Cauchy integral principal values. To overcome the difficulties of a nonlinear curvilinear solid boundary character and free water surface not being known a priori, the boundary integral equations are transformed to the physical plane by substituting the integral variables. As such, the proposed approach has the following advantages: (1) the angle of the curvilinear solid boundary as well as the location of free water surface (initially assumed) is a known function of coordinates in physical plane; and (2) the meshes can be flexibly assigned on the solid and free water surface boundaries along which the integration is performed. This avoids the difficulty of the traditional potential flow theory, which seeks a function to conformally map the geometry in physical plane onto an auxiliary plane. Furthermore, rough bed friction-induced energy loss is estimated using the Darcy-Weisbach equation and is solved together with the boundary integral equations using the proposed iterative method. The method has no stringent requirement for initial free-water surface position, while traditional potential flow methods usually have strict requirement for the initial free-surface profiles to ensure that the numerical computation is stable and convergent. Several typical open-channel flows have been calculated with high accuracy and limited computational time, indicating that the proposed method has general suitability for open-channel flows with complex geometry

    The generalized Hamiltonian model for the shafting transient analysis of the hydro turbine generating sets.

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    yesTraditional rotor dynamics mainly focuses on the steady- state behavior of the rotor and shafting. However, for systems such as hydro turbine generating sets (HTGS) where the control and regulation is frequently applied, the shafting safety and stabilization in transient state is then a key factor. The shafting transient state inevitably involves multiparameter domain, multifield coupling, and coupling dynamics. In this paper, the relative value form of the Lagrange function and its equations have been established by defining the base value system of the shafting. Takingthe rotation angle and the angular speed of the shafting as a link, the shafting lateral vibration and generator equations are integrated into the framework of generalized Hamiltonian system. The generalized Hamiltonian control model is thus established. To make the model more general, additional forces of the shafting are taken as the input excitation in proposed model. The control system of the HTGS can be easily connected with the shafting model to form the whole simulation system of the HTGS. It is expected that this study will build a foundation for the coupling dynamics theory using the generalized Hamiltonian theory to investigate coupling dynamic mechanism among the shafting vibration, transient of hydro turbine generating sets, and additional forces of the shafting.National Natural Science Foundation of China under Grant Nos. 51179079 and 5083900

    Combustion and emission performance of swirling-flow single trapped vortex combustor

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    The swirling-flow single trapped vortex combustor (SSTVC) provides a new design approach for aviation engine combustors, showing promising potential for achieving high-temperature rises or low emissions. However, current research on it is limited to demonstrating its feasibility and lacks further investigation into its combustion organization, combustion, and emission characteristics. In this study, experiments are conducted to investigate the flow field, combustion, and emission characteristics of the SSTVC under 5 inlet reference velocities and 3 inlet temperatures. As the inlet reference velocities increase from 9.96 m/s to 14.95 m/s, noticeable changes are observed in the backflow velocity of the cavity, increasing from 33.8 m/s to 48.3 m/s. By combining the flow field structure and flame features, the combustion organization of the SSTVC is summarized. With an increase in the inlet reference velocities, the combustion efficiency remains almost unchanged initially at 95.75% and then increases to 96.55%. The outlet temperature distribution improves, with the pattern factor value decreasing from 0.23 to 0.14. The carbon monoxide emissions initially increased from 94.91 to 99.89 and then decreased to 88.80, the total unburned hydrocarbons decreased from 23.22 to 16.03, and the nitrogen oxide emissions showed no significant change. With an increase in the inlet temperature, all parameters improved, with the combustion efficiency increasing from 93.71% to 96.61%, the pattern factor decreasing from 0.26 to 0.12, and all emission parameters decreasing as well.This work was supported by the National Science and Technology Major Project. Yuxi gratefully acknowledges the financial support from the China Scholarship Council, NO.202206830122
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