VH-RELATED IDIOTOPES DETECTED BY SITE-DIRECTED MUTAGENESIS

The function of the CD4 cell surface protein as coreceptor on T helper lymphocytes and as receptor for HIV makes this glycoprotein a prime target for an immune intervention with mAb. A detailed understanding of the structural determinants on the therapeutic CD4 mAb that are involved in Ag binding or are recognized by anti-idiotypic mAb (anti-Id) may be important for designing antibodies with optimal therapeutic efficacy. Seven anti-Id raised against the CD4 mAb M-T310 were selected from a large panel with the intention to obtain CD4 mimicking structures with specificity foHr IV gp120. The selected anti-Id did not reacwt ith other CDCspecific mAb cross-blocking M-T310. Among these, mAb MT404, although having the same L chain as M-T310 and a VH region sequence differing onlya t 14 amino acid positions, was not recognized by the anti-Id. MT310 H chain complexed with the J558L L chain reacted with all anti-Id, thus demonstrating that the recognized idiotopes are located within the VH region. To identify the idiotopes of M-T310 seen by the anti-Id, variants of M-T404 containing one or more of the M-T3 1 O-derived substitutions were generated by oligonucleotide-directed mutagenesis. The reactivity pattern of the mutant proteins with the anti-Id demonstrated that the idiotopes reside within the complementarity determining region (CDR) 2 and CDR3 loops of the VH region. A major idiotope was definebdy a single amino acid in CDR2 that was recognized by three anti-Id, whereas the four other anti-Id reacted with determinants of CDR3. Although the performed amino acid substitutions did influence the Id recognition, Ag binding was not significantly affected, suggesting that none of the anti-Id can be considered as a mimicry of the CD4 A

Comment on Ds∗→Dsπ0D_s^* \to D_s \pi^0 Decay

We calculate the rate for $D_s^* \rightarrow D_s \pi^0$ decay using Chiral Perturbation Theory. This isospin violating process results from $\pi^0$ - $\eta$ mixing, and its amplitude is proportional to $(m_d - m_u)/\bigl(m_s-(m_u+m_d)/2 \bigr)$. Experimental information on the branching ratio for $D_s^* \rightarrow D_s \pi^0$ can provide insight into the pattern of $SU(3)$ violation in radiative $D^*$ decays.Comment: 7 pages with 2 figures not included but available upon request, CALT-68-191

Advanced aeroservoelastic stabilization techniques for hypersonic flight vehicles

Advanced high performance vehicles, including Single-Stage-To-Orbit (SSTO) hypersonic flight vehicles, that are statically unstable, require higher bandwidth flight control systems to compensate for the instability resulting in interactions between the flight control system, the engine/propulsion dynamics, and the low frequency structural modes. Military specifications, such as MIL-F-9490D and MIL-F-87242, tend to limit treatment of structural modes to conventional gain stabilization techniques. The conventional gain stabilization techniques, however, introduce low frequency effective time delays which can be troublesome from a flying qualities standpoint. These time delays can be alleviated by appropriate blending of gain and phase stabilization techniques (referred to as Hybrid Phase Stabilization or HPS) for the low frequency structural modes. The potential of using HPS for compensating structural mode interaction was previously explored. It was shown that effective time delay was significantly reduced with the use of HPS; however, the HPS design was seen to have greater residual response than a conventional gain stablized design. Additional work performed to advance and refine the HPS design procedure, to further develop residual response metrics as a basis for alternative structural stability specifications, and to develop strategies for validating HPS design and specification concepts in manned simulation is presented. Stabilization design sensitivity to structural uncertainties and aircraft-centered requirements are also assessed

Microscopic investigation of phonon modes in SiGe alloy nanocrystals

Phonon modes in spherical silicon germanium alloy (SiGe) nanocrystals containing up to 1147 atoms (3.6 nm) have been investigated as a function of the Si concentration. Microscopic details of phonon modes, including phonon frequencies and vibrational amplitudes, phonon density-of-states are calculated directly from the dynamic matrices. In particular, the dependence of phonon frequency on the configuration (such as a different ratio of Si to Ge atoms), and location (surface or interior) of clusters of atoms in SiGe alloy nanocrystals have been investigated. Low frequency surface phonons that are related to the spheroidal and torsional modes of a continuum sphere are identified and their frequency dependence on alloy concentration elucidated. The calculated results are compared with measured Raman spectra in bulk, thin films, and superlattices of SiGe alloy reported in the literature. Insights into the behavior of Raman peaks usually identified as Ge-Ge, Si-Si, and Ge-Si optical phonon modes are presented

Theoretical investigation of the surface vibrational modes in germanium nanocrystals

We have used a microscopic lattice dynamical model to study phonon modes in germanium (Ge) NC with size varying between 47 to 7289 atoms (diametersimilar to6.8 nm). By separating these atoms into bulk and surface atoms we have found that surface modes can exist in Ge NC both at low frequencies (\u3c50\u3ecm(-1)) and at high frequency (similar to260 cm(-1)). The latter mode is a resonant mode which occurs in the pseudogap between the acoustic and optical phonon branches in bulk Ge. From the low frequency surface modes we have been able to reconstruct the spheroidal and torsional Lamb modes which have been used to interpret experimental results. Finally, we found that the Lamb model starts to deviate from the lattice dynamical results for Ge NC with diameternm

Chiral transition and deconfinement transition in QCD with the highly improved staggered quark (HISQ) action

We report preliminary results on the chiral and deconfinement aspects of the QCD transition at finite temperature using the Highly Improved Staggered Quark (HISQ) action on lattices with temporal extent of N_{\tau}=6 and 8. The chiral aspects of the transition are studied in terms of quark condensates and the disconnected chiral susceptibility. We study the deconfinement transition in terms of the strange quark number susceptibility and the renormalized Polyakov loop. We made continuum estimates for some quantities and find reasonably good agreement between our results and the recent continuum extrapolated results obtained with the stout staggered quark action.Comment: Talk presented by P. Petreczky at workshop Dense Matter 2010, April 6-9, Stellenbosch, South Africa, to be published in the proceeding

Chiral Estimates of Strong CP Violation Revisited

The effects of the CP violating $\theta$ term in the QCD Lagrangian upon low energy hadronic phenomenology are reconsidered. Strong CP violating interactions among Goldstone bosons and octet baryons are incorporated into an effective chiral Lagrangian framework. The $\theta$ term's impact upon the decays $\eta\to\pi\pi$ and $\pi^0\to\gamma\gamma$ is then investigated but found to be extremely small. A refined model independent estimate of nonanalytic contributions to the neutron electric dipole moment is also determined using velocity dependent Baryon Chiral Perturbation Theory. We obtain the approximate upper bound $|\theta| < 4.5 \times 10^{-10}$.Comment: 11 pages with 3 figures not included but available upon request, CALT-68-184