Successful cervicothoracic esophageal stricture treatment with partial sternectomy and a pedicled TAAP flap: A case report

Postoperative benign esophageal anastomotic leakage and stenosis are common complications after esophagectomy. Treatment options for anastomosis stenosis include endoscopic mechanical dilation, dilation-combined steroid injection, incisional therapy, stent placement, and self-bougienage. However, long-segmental cervicothoracic esophageal stenosis and cutaneous fistula are always refractory to conservative treatments and are clinically challenging. When lesions extend well below the thoracic inlet, transthoracic esophagectomy and alimentary canal reconstruction seem to be the common choice but are susceptible to perioperative mortality and donor-site sequelae, especially for patients with poor health conditions. In this report, we present a novel surgical approach for cervicothoracic esophageal stenosis and fistula via partial sternectomy and reconstruction with a pedicled thoracoacromial artery perforator flap. No recurrence or complications occurred throughout 3 months of follow-up. This case study adds new perspectives to the treatment of anastomotic stenosis

The complete plastid genome of Camellia Octopetala (Theaceae)

Camellia octopetala is a native oil tree species in the south of China and is also a unique natural Chinese woody edible oil species. In the study, the complete plastid genome was assembled and annotated, the genome full-length is 156,615 bp, contains a large single-copy (LSC) region with 86,273 bp, a small single-copy (SSC) region with 18,410 bp, two invert repeats (IR) regions with 25,966 bp. The plastid genome contains 135 genes, 90 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis shows C. octopetala sister to C. crapnelliana and embedded in Camellia

The valid publication of Salix suchowensis (Salicaceae)

The nomenclatural problems of Salix suchowensis have been addressed by different authors with varying opinions. However, these efforts were flawed by a lack of observation of relevant specimens. Accordingly, we carefully checked relevant publications and specimens both through internet databases and herbarium visits. Here, we thoroughly review the nomenclatural history of Salix suchowensis in light of the new definition of a gathering in the Shenzhen Code. We conclude that this name was validly published in the original publication in 1963. Furthermore, a lectotype is designated for the precise application of the name. We hope this article will offer guidance for interpreting similar cases

The complete chloroplast genome of Phoebe zhennan

Phoebe zhennan is a large tree with straight trunk and grey-brown or grey-yellowish villous as rare wood. In the study, the complete chloroplastid genome was assembled and annotated, its full length of 152,831 bp, include large single-copy (LSC) region of 93,753 bp, small single-copy (SSC) region of 18,928 bp, a pair of invert repeats (IR) regions of 20,075 bp. Plastid genome contains 127 genes, 81 protein-coding genes, 36 tRNA genes and 8 rRNA genes. Phylogenetic analysis suggested P. zhennan is sister to Phoebe sheareri and embed in Lauraceae

TMT-Based Quantitative Proteomic Analysis Reveals the Crucial Biological Pathways Involved in Self-Incompatibility Responses in Camellia oleifera

Camellia oleifera is a valuable woody oil plant belonging to the Theaceae, Camellia oil extracted from the seed is an excellent edible oil source. Self-incompatibility (SI) in C. oleifera results in low fruit set, and our knowledge about the mechanism remains limited. In the present study, the Tandem mass tag (TMT) based quantitative proteomics was employed to analyze the dynamic change of proteins response to self- and cross-pollinated in C. oleifera. A total of 6,616 quantified proteins were detected, and differentially abundant proteins (DAPs) analysis identified a large number of proteins. Combined analysis of differentially expressed genes (DEGs) and DAPs of self- and cross-pollinated pistils based on transcriptome and proteome data revealed that several candidate genes or proteins involved in SI of C. oleifera, including polygalacturonase inhibitor, UDP-glycosyltransferase 92A1-like, beta-D-galactosidase, S-adenosylmethionine synthetase, xyloglucan endotransglucosylase/hydrolase, ABC transporter G family member 36-like, and flavonol synthase. Venn diagram analysis identified 11 proteins that may participate in pollen tube growth in C. oleifera. Our data also revealed that the abundance of proteins related to peroxisome was altered in responses to SI in C. oleifera. Moreover, the pathway of lipid metabolism-related, flavonoid biosynthesis and splicesome were reduced in self-pollinated pistils by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In summary, the results of the present study lay the foundation for learning the regulatory mechanism underlying SI responses as well as provides valuable protein resources for the construction of self-compatibility C. oleifera through genetic engineering in the future

Biochar Impacts on Acidic Soil from Camellia Oleifera Plantation: A Short-Term Soil Incubation Study

Nowadays, biochar is increasingly used widely as an important soil amendment to enhance soil nutrients availability. Therefore, we investigated the effect of C.oleifera shell biochar (CSB) on C.oleifera plantation soils to provide evidence that C. oleifera shell as a raw material in biochar has great potential to be a soil amendment. For this, a short-term incubation experiment was conducted in controlled conditions to evaluate the effects of CSB application on two soil chemical properties, microbial biomass, and enzymatic activity. We compared two acidic soils, mixed with CSB of three pyrolysis temperatures (300, 500, and 700 °C), and two application rates (3% and 5% (w/w)), incubated for 180 days. The results showed that the soil pH, total P (TP), and available P (AP) significantly increased under 5CSB700 in two soils, and indicated CSB application rate and pyrolysis temperature had a significant impact on soil pH, TP, and AP (p < 0.05). CSB application also significantly increased the total inorganic P in two soils and presented a significantly positive correlation with soil pH, TP, and AP under redundancy analysis. The results suggested that CSB application has a variable effect on soil enzymatic activity, microbial biomass C (MBC), and microbial biomass P (MBP) on average, while it increased the soil microbial biomass N (MBN) in both soils. We concluded that CSB could be a soil amendment to increase soil nutrients of C.oleifera plantation soils. Before the application of biochar to C.oleifera plantation forest soils, long-term studies are required to assess the effects of biochar under field conditions and its promoting effect on the growth of C. oleifera

The Complete Chloroplast Genome of Guadua angustifolia and Comparative Analyses of Neotropical-Paleotropical Bamboos

To elucidate chloroplast genome evolution within neotropical-paleotropical bamboos, we fully characterized the chloroplast genome of the woody bamboo Guadua angustifolia. This genome is 135,331 bp long and comprises of an 82,839-bp large single-copy (LSC) region, a 12,898-bp small single-copy (SSC) region, and a pair of 19,797-bp inverted repeats (IRs). Comparative analyses revealed marked conservation of gene content and sequence evolutionary rates between neotropical and paleotropical woody bamboos. The neotropical herbaceous bamboo Cryptochloa strictiflora differs from woody bamboos in IR/SSC boundaries in that it exhibits slightly contracted IRs and a faster substitution rate. The G. angustifolia chloroplast genome is similar in size to that of neotropical herbaceous bamboos but is ~3 kb smaller than that of paleotropical woody bamboos. Dissimilarities in genome size are correlated with differences in the lengths of intergenic spacers, which are caused by large-fragment insertion and deletion. Phylogenomic analyses of 62 taxa yielded a tree topology identical to that found in preceding studies. Divergence time estimation suggested that most bamboo genera diverged after the Miocene and that speciation events of extant species occurred during or after the Pliocene

The complete chloroplast genome of Semiliquidambar cathayensis (Hamamelidaceae)

Semiliquidambar cathayensis is a semi-evergreen broad-leaved tree species distributed in southern China. In 1999, it was approved and published as a national secondary protected plant. We obtained the complete chloroplast genome sequence of S. cathayensis by Illumina sequencing data. The complete chloroplast sequence is 160,430 bp, include large single-copy (LSC) region of 88,991 bp, small single-copy (SSC) region of 18,917 bp, and a pair of invert repeats (IR) regions of 26,261 bp. Plastid genome contain 133 genes, 86 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis showed that S. cathayensis is closely related to Liquidambar formosana

The complete chloroplast genome of Altingia chinensis (Hamamelidaceae)

Altingia chinensis is an important native broad-leaved tree in southern China. Its wood can be used as an excellent edible fungus culture medium, in addition, aromatic oil can be extracted for medicine and spices. Its full-length chloroplast genome is 160,410 bp, including a large single copy (LSC) region of 88,936 bp, a small single copy (SSC) region of 18,917 bp, and a pair of inverted repeat (IR) regions of 26,274 bp. Furthermore, we also found 132 genes, 86 protein-coding genes, 37 tRNA genes, and 8 rRNA genes in the chloroplast genome of Altingia chinensis. Phylogenetic analysis shows that Altingia chinensis is most closely related to Liquidambar formosana and Semiliquidambar cathayensis. This study can provide basic data for research on molecular-assisted breeding of Altingia chinensis,and phylogeny of Hamamelidaceae

Complete chloroplast genome sequence of Acacia crassicarpa (Fabaceae)

Acacia crassicarpa (Fabaceae), a nitrogen-fixing tree species, is critically important for coastal protection in southeast China. In this study, we report the complete chloroplast genome sequence of A. crassicarpa, with a length of 176,493 bp. It contains a pair of inverted repeats (IR 39,851 bp), a large single-copy region (LSC 91,869 bp), and a small single-copy region (SSC 4,922 bp). The complete genome comprises 138 genes, including 93 protein-coding genes, 37 tRNA, and 8 rRNA genes. Our phylogenetic analysis reveals that A. crassicarpa is closely related to A. podalyriifolia and A. dealbata