A composite open resonator for compact X-ray source

The results of calculation of the finesse of a composite open resonator for compact X-ray source are presented. The region of the resonator parameters has been found where the finesse is changed unessentially.Comment: 2 pages, LaTe

Effects of Phosphorylation on Phosphoenolpyruvate Carboxykinase from the C4 Plant Guinea Grass

In the C4 plant Guinea grass (Panicum maximum), phosphoenolpyruvate carboxykinase (PEPCK) is phosphorylated in darkened leaves and dephosphorylated in illuminated leaves. To determine whether the properties of phosphorylated and non-phosphorylated PEPCK were different, PEPCK was purified to homogeneity from both illuminated and darkened leaves. The final step of the purification procedure, gel filtration chromatography, further separated phosphorylated and non-phosphorylated forms. In the presence of a high ratio of ATP to ADP, the non-phosphorylated enzyme had a higher affinity for its substrates, oxaloacetate and phosphoenolpyruvate. The activity of the non-phosphorylated form was up to 6-fold higher when measured at low substrate concentrations. Comparison of proteoloytically cleaved PEPCK from Guinea grass, which lacked its N-terminal extension, from yeast (Saccharomyces cerevisiae), which does not possess an N-terminal extension, and from the C4 plant Urochloa panicoides, which possesses an N-terminal extension but is not subject to phosphorylation, revealed similar properties to the non-phosphorylated full-length form from Guinea grass. Assay of PEPCK activity in crude extracts of Guinea grass leaves, showed a large difference between illuminated and darkened leaves when measured in a selective assay (a low concentration of phosphoenolpyruvate and a high ratio of ATP to ADP), but there was no difference under assay conditions used to estimate maximum activity. Immunoblots of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels showed no difference in the abundance of PEPCK protein in illuminated and darkened leaves. There were no light/dark differences in activity detected in maize (Zea mays) leaves, in which PEPCK is not subject to phosphorylation

Phosphoenolpyruvate Carboxykinase Assayed at Physiological Concentrations of Metal Ions Has a High Affinity for CO2

The effect of Mn2+/Mg2+ concentration on the activity of intact, homogeneous phosphoenolpyruvate carboxykinase (PEPCK) from leaves of the C4 grass, Guinea grass (Panicum maximum), have been investigated. Assay conditions were optimized so that PEPCK activity could be measured at concentrations of Mn2+/Mg2+ similar to those found in the cytosol (low micromolar Mn2+ and millimolar Mg2+). PEPCK activity was totally dependent on Mn2+ and was activated at low micromolar concentrations of Mn2+ by millimolar concentrations of Mg2+. Therefore, at physiological concentrations of Mn2+, PEPCK has a requirement for Mg2+. Assay at physiological concentrations of Mn2+/Mg2+ led to a marked decrease in its affinity for ATP and a 13-fold increase in its affinity for CO2. The Km (CO2) was further decreased by assay at physiological ATP to ADP ratios, reaching values as low as 20 μM CO2, comparable with the Km (CO2) of ribulose 1,5-bisphosphate carboxylase-oxygenase. This means that PEPCK will catalyze a reversible reaction and that it could operate as a carboxylase in vivo, a feature that could be particularly important in algal CO2-concentrating systems

Overcoming observability problems in distributed test architectures

This paper investigates conditions that must be satisfied by an FSM for the existence of input sequences that can be applied in a distributed test architecture without encountering controllability and observability problems and without using external coordination messages. Such conditions have two potential values. First, they can be used to determine whether we require coordination messages and thus a network that connects the testers. Second, if we wish to avoid the use of coordination messages in testing then these conditions can be seen as testability conditions that can inform the design process. Results given in this paper differ from those in the following ways. First, the conditions are strictly weaker than those in since we are less restrictive in the ways we achieve our goals. Second, only considered observability problems; we consider both controllability and observability problems. In addition, only considered a particular type of observability problem and we generalize this. Finally, we investigate the situation in which we need only add input sequences to complement a given test/checking sequence ρ and prove that the conditions for this problem are equivalent to those for the original problem

Overcoming controllability problems in distributed testing from an input output transition system

This is the Pre-print version of the Article. The official published version can be accessed from the link below - Copyright @ 2012 Springer VerlagThis paper concerns the testing of a system with physically distributed interfaces, called ports, at which it interacts with its environment. We place a tester at each port and the tester at port p observes events at p only. This can lead to controllability problems, where the observations made by the tester at a port p are not sufficient for it to be able to know when to send an input. It is known that there are test objectives, such as executing a particular transition, that cannot be achieved if we restrict attention to test cases that have no controllability problems. This has led to interest in schemes where the testers at the individual ports send coordination messages to one another through an external communications network in order to overcome controllability problems. However, such approaches have largely been studied in the context of testing from a deterministic finite state machine. This paper investigates the use of coordination messages to overcome controllability problems when testing from an input output transition system and gives an algorithm for introducing sufficient messages. It also proves that the problem of minimising the number of coordination messages used is NP-hard

Modelling and control of a high redundancy actuator

The high redundancy actuation concept is a completely new approach to fault tolerance, and it is important to appreciate that it provides a transformation of the characteristics of actuators so that the actuation performance (capability) degrades slowly rather than suddenly failing, even though individual elements themselves fail. This paper aims to demonstrate the viability of the concept by showing that a highly redundant actuator, comprising a relatively large number of actuation elements, can be controlled in such a way that faults in individual elements are inherently accommodated, although some degradation in overall performance will inevitably be found. The paper introduces the notion of fault-tolerant systems and the highly redundant actuator concept. Then a model for a two by two configuration with electro-mechanical actuation elements is derived. Two classical control approaches are then considered based on frequency domain techniques. Finally simulation results under a number of faults show the viability of the approach for fault accommodation without re-configuratio

Strange matrix elements of the nucleon

Results for the disconnected contributions to matrix elements of the vector current and scalar density have been obtained for the nucleon from the Wilson action at beta=6 using a stochastic estimator technique and 2000 quenched configurations. Various methods for analysis are employed and chiral extrapolations are discussed.Comment: Lattice2002(matrixel), 3 pages, 3 figure

Lattice-Boltzmann Method for Geophysical Plastic Flows

We explore possible applications of the Lattice-Boltzmann Method for the simulation of geophysical flows. This fluid solver, while successful in other fields, is still rarely used for geotechnical applications. We show how the standard method can be modified to represent free-surface realization of mudflows, debris flows, and in general any plastic flow, through the implementation of a Bingham constitutive model. The chapter is completed by an example of a full-scale simulation of a plastic fluid flowing down an inclined channel and depositing on a flat surface. An application is given, where the fluid interacts with a vertical obstacle in the channel.Comment: in W. Wu, R.I. Borja (Edts.) Recent advances in modelling landslides and debris flow, Springer Series in Geomechanics and Geoengineering (2014), ISBN 978-3-319-11052-3, pp. 131-14

Modeling the evolution of chorus waves into plasmaspheric hiss

Plasmaspheric hiss (PH) is a band-limited, incoherent whistler mode emission found predominantly in the plasmasphere or high-density plasma regions in the near-Earth space environment. Since its discovery in the late 1960s, PH has been recognized as playing an important role in shaping the structure and dynamics of the Earth's electron radiation belts and creating the slot region that separates the inner and outer belts. However, the origin of PH has been a topic of intense debate for over four decades. Here we present a model for the origin of PH that involves the evolution of chorus waves into the PH spectrum. We perform extensive ray tracing using the HOTRAY code and calculate Landau damping using newly developed suprathermal flux maps from THEMIS observations, that are L and magnetic local time dependent, for both inside and outside the plasmasphere. Our results show remarkable consistency with the observed statistical characteristics of hiss, including the day/night asymmetry in wave power, frequency spectrum, geomagnetic control of PH, quasi-parallel equatorial wave normal angles, and confinement within the plasmasphere. Our model also reproduces ancillary features such as exohiss and extremely low frequency (ELF) hiss and might be related to a previously reported phenomenon called lower hybrid resonance duct trapping in the ionosphere. A detailed analysis of ray morphologies shows a separation into four distinct groups, which correspond to (1) rays that are trapped at the plasmapause, (2) PH rays, (3) ELF hiss rays, and (4) rays that represent the bulk of the chorus ray power