Multi-electrolyte-step anodic aluminum oxide method for the fabrication of self-organized nanochannel arrays

Nanochannel arrays were fabricated by the self-organized multi-electrolyte-step anodic aluminum oxide [AAO] method in this study. The anodization conditions used in the multi-electrolyte-step AAO method included a phosphoric acid solution as the electrolyte and an applied high voltage. There was a change in the phosphoric acid by the oxalic acid solution as the electrolyte and the applied low voltage. This method was used to produce self-organized nanochannel arrays with good regularity and circularity, meaning less power loss and processing time than with the multi-step AAO method

Higher-order effects on the incompressibility of isospin asymmetric nuclear matter

Analytical expressions for the saturation density as well as the binding energy and incompressibility at the saturation density of asymmetric nuclear matter are given exactly up to 4th-order in the isospin asymmetry delta =(rho_n - rho_p)/rho using 11 characteristic parameters defined at the normal nuclear density rho_0. Using an isospin- and momentum-dependent modified Gogny (MDI) interaction and the SHF approach with 63 popular Skyrme interactions, we have systematically studied the isospin dependence of the saturation properties of asymmetric nuclear matter, particularly the incompressibility $K_{sat}(\delta )=K_{0}+K_{sat,2}\delta ^{2}+K_{sat,4}\delta ^{4}+O(\delta ^{6})$ at the saturation density. Our results show that the magnitude of the high-order $K_{sat,4}$ parameter is generally small compared to that of the K_{\sat,2} parameter. The latter essentially characterizes the isospin dependence of the incompressibility at the saturation density and can be expressed as $K_{sat,2}=K_{sym}-6L-\frac{J_{0}}{K_{0}}L$, Furthermore, we have constructed a phenomenological modified Skyrme-like (MSL) model which can reasonably describe the general properties of symmetric nuclear matter and the symmetry energy predicted by both the MDI model and the SHF approach. The results indicate that the high-order $J_{0}$ contribution to $K_{sat,2}$ generally cannot be neglected. In addition, it is found that there exists a nicely linear correlation between $K_{sym}$ and $L$ as well as between $J_{0}/K_{0}$ and $K_{0}$. These correlations together with the empirical constraints on $K_{0}$, $L$, $E_{sym}(\rho_{0})$ and the nucleon effective mass lead to an estimate of $K_{sat,2}=-370\pm 120$ MeV.Comment: 61 pages, 12 figures, 6 Tables. Title changed a little and results of several Skyrme interactions updated. Accepted version to appear in PR

KINETIC DIFFERENCES IN LOWER EXTREMITY BETWEEN BASEBALL PITCHING FROM PITCHER’S MOUND AND FLAT-GROUND

The purpose of this study was to investigate the kinetic differences in lower extremity between pitching from a mound and flat-ground. A motion capture system and two force plates were used simultaneously to collect the dynamic data of 8 baseball male pitchers. The results revealed that pitching from the mound generated higher propulsive force at the trailing leg as well as greater braking force and vertical ground reaction force at the lead leg (p< .05). The trailing leg in the mound condition generated greater knee posterior joint force while the lead leg had greater axial joint force at ankle and knee, as well as greater extension moment at ankle, knee and hip (p< .05). It was concluded that pitching from the mound generated higher ground reaction force, which resulted in higher joint forces and moments and thus might increase stresses at lower extremity

Probing the DNA kink structure induced by the hyperthermophilic chromosomal protein Sac7d

Sac7d, a small, abundant, sequence-general DNA-binding protein from the hyperthermophilic archaeon Sulfolobus acidocaldarius, causes a single-step sharp kink in DNA (∼60°) via the intercalation of both Val26 and Met29. These two amino acids were systematically changed in size to probe their effects on DNA kinking. Eight crystal structures of five Sac7d mutant–DNA complexes have been analyzed. The DNA-binding pattern of the V26A and M29A single mutants is similar to that of the wild-type, whereas the V26A/M29A protein binds DNA without side chain intercalation, resulting in a smaller overall bending (∼50°). The M29F mutant inserts the Phe29 side chain orthogonally to the C2pG3 step without stacking with base pairs, inducing a sharp kink (∼80°). In the V26F/M29F-GCGATCGC complex, Phe26 intercalates deeply into DNA bases by stacking with the G3 base, whereas Phe29 is stacked on the G15 deoxyribose, in a way similar to those used by the TATA box-binding proteins. All mutants have reduced DNA-stabilizing ability, as indicated by their lower T(m) values. The DNA kink patterns caused by different combinations of hydrophobic side chains may be relevant in understanding the manner by which other minor groove-binding proteins interact with DNA

Device Integrity of Drug-eluting Depot Stent for Smart Drug Delivery

Atherosclerosis, or hardening of the arteries, is a condition in which plaque, made of cholesterol, fatty substances, cellular waste products, calcium, and fibrin, builds up inside the arteries. A metallic stent is a small mesh tube that is used to treat these narrowed arteries such as coronary artery diseases. The drug-eluting stent has a metallic stent platform coated with drug-polymer mix and has been shown to be superior to its metallic stent counterpart in reducing restenosis. In the past few years, a novel variation of the drug-eluting stent with micro-sized drug reservoirs (depot stent) has been introduced to the market. It allows smart programmable drug delivery with spatial/temporal control and has potential advantages over conventional stents. The drug-polymer mix compound can be altered from one reservoir to the next, allowing a highly-controlled release of different medications. For example, this depot stent concept can be applied in the renal indication for potential treatment of both renal artery stenosis (upstream) and its associated kidney diseases (downstream) simultaneously. However, the creation of such drug reservoirs on the stent struts inevitably compromises its mechanical integrity. In this study, the effects of these drug reservoirs on stent key clinical attributes were systematically investigated. We developed finite element models to predict the mechanical integrity of a balloon-expandable stent at various stages of its function life such as manufacturing and acute deployment, as well as the stent radial strength and chronic fatigue life. Simulation results show that (1) creating drug reservoirs on a stent strut could impact the stent fatigue resistance to certain degrees; (2) drug reservoirs on the high stress concentration regions led to much greater loss in all key clinical attributes than reservoirs on other locations; (3) reservoir shape change resulted in little differences in all key clinical attributes; and (4) for the same drug loading capacity, larger and fewer reservoirs yielded higher fatigue safety factor. These results can help future stent designers to achieve the optimal balance of stent mechanical integrity and smart drug delivery, thereby opening up a wide variety of new opportunities for disease treatments. We also proposed an optimized depot stent with tripled drug capacity and acceptable marginal trade-off in key clinical attributes when compared to the current drug-eluting stents. This depot stent prototype was manufactured for the demonstration of our design concept