Sensitivity of IFN-γ Release Assay to Detect Latent Tuberculosis Infection Is Retained in HIV-Infected Patients but Dependent on HIV/AIDS Progression

BACKGROUND: Detection and treatment of latent TB infection (LTBI) in HIV infected individuals is strongly recommended to decrease morbidity and mortality in countries with high levels of HIV. OBJECTIVE: To assess the validity of a newly developed in-house ELISPOT interferon-gamma release assay (IGRA) for the detection of LTBI amongst HIV infected individuals, in comparison with the Tuberculin Skin Test (TST). METHODOLOGY/PRINCIPAL FINDINGS: ESAT6/CFP10 (EC) ELISPOT assays were performed, together with a TST, in 285 HIV infected individuals recruited in HIV clinics in Dakar, Senegal, who had no signs of active TB at time of enrolment. Thirty eight of the subjects (13.3%) failed to respond to PHA stimulation and were excluded from the analysis. In the 247 remaining patients, response to PHA did not vary according to CD4 cell count categories (p = 0.51). EC ELISPOT was positive in 125 (50.6%) subjects, while 53 (21.5%) had a positive TST. Concordance between EC ELISPOT and TST was observed in 151 patients (61.1%) (kappa = 0.23). The proportion of subjects with a positive response to the EC ELISPOT assay decreased with declining CD4 counts (p trend = 0.001), but were consistently higher than the proportion of TST responders. In multivariate analysis, the risk of being EC-ELISPOT positive in HIV infected individuals was associated with age, CD4 count and HIV-1 strain. CONCLUSION: Our study indicates that IGRAs using M. tuberculosis specific antigens are likely to retain their validity for the diagnosis of LTBI among HIV positive individuals, but may be impaired by T-cell anergy in severely immuno-suppressed individuals

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance.

Investment in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing in Africa over the past year has led to a major increase in the number of sequences that have been generated and used to track the pandemic on the continent, a number that now exceeds 100,000 genomes. Our results show an increase in the number of African countries that are able to sequence domestically and highlight that local sequencing enables faster turnaround times and more-regular routine surveillance. Despite limitations of low testing proportions, findings from this genomic surveillance study underscore the heterogeneous nature of the pandemic and illuminate the distinct dispersal dynamics of variants of concern-particularly Alpha, Beta, Delta, and Omicron-on the continent. Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve while the continent faces many emerging and reemerging infectious disease threats. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Beyond Aid

Post debt relief, the number of African countries considering accessing international capital markets, often to fund large infrastructure projects, is increasing. Potential risks of capital inflows are well known but the literature offers little help to estimate the cost of borrowing internationally for the first time. This paper proposes a two-step approach to estimate the sovereign credit rating and interest rate cost of a country considering borrowing externally. Estimates can be used to assess the costs and benefits of different financing options. The method can also be used to construct foreign currency as well as domestic local currency yield curves.External borrowing;External financing;Capital inflows;International bond markets;Infrastructure;Low-income developing countries;capital markets, bond, international capital markets, market bond, international capital, bond spreads, bonds, bond yield, sovereign bond, emerging market bond, credit rating, international bonds, sovereign bonds, current account balance, cost of capital, emerging market bonds, risk aversion, corporate bonds, corporate bond, inflation rate, capital flows, forward markets, bond spread, financial stability, capital outflows, international bond, denominated bonds, high yield bond, global bond index, treasury bonds, global bond, international interest rates, stock index, credit rationing, bond index, international finance, capital markets borrowing, credit rating agencies, yields on bonds, bond issuers, credit constraint, current account deficit, outstanding bonds, currency of denomination, index option, mature markets, underdeveloped capital markets, stock index option, bond markets

Growth in Africa Under Peace and Market Reforms

Economic stagnation in Sub-Saharan Africa (SSA) has led several economists to question the region’s ability to attain sustained economic growth, some of them arguing for the need to shift away from natural resource - based exports. Yet, we find that low growth has not been common to all SSA countries and that those that achieved political stability and significantly liberalized their economies experienced high growth in income per capita, as high as ASEAN-5 countries. This group of SSA countries attained high growth while maintaining their specialization in natural resource exports. Our analysis also rejects the hypothesis of reverse causality: that good growth performance allowed countries to attain political stability or liberalize their economies.Structural adjustment;Economic growth;Economic reforms;Natural resources;economic liberalization, terms of trade, growth rates, growth rate, commodity prices, gdp growth, gdp per capita, current account balance, exporting countries, trade growth, trade liberalization, trade openness, real gdp, capital formation, gross capital formation, low trade, world economy, international trade, growth in income per capita, trade flows, political economy, pro-poor growth, commodity exporters, barriers to entry, prudential supervision, income distribution, commercial policy, competitive industry, growth diagnostics, current account deficit, balance of payments crises, poverty alleviation, balance of payments, economic convergence, competitive market, oil prices

Formation and Transport of a Saharan Dust Plume in Early Summer

This research studies the capability of the Weather Research and Forecasting model coupled with the Chemistry/Aerosol module (WRF-Chem) with and without parametrization to reproduce a dust storm, which was held on 27th June 2018 over Sahara region. The authors use satellite observations and ground-based measurements to evaluate the WRF-Chem simulations. The sensitivities of WRF-Chem Model are tested on the replication of haboob features with a tuned GOCART aerosol module. Comparisons of simulations with satellite and ground-based observations show that WRF-Chem is able to reproduce the Aerosol Optical Depth (AOD) distribution and associated changes of haboob in the meteorological fields with temperature drops of about 9 °C and wind gust 20 m·s–1. The WRF-Chem Convection-permitting model (CPM) shows strong 10-meter winds induced a large dust emission along the leading edge of a convective cold pool (LECCP). The CPM indicates heavy dust transported over the West African coast (16°W-10°W; 6°N-21°N) which has a potential for long-distance travel on 27th June between 1100 UTC and 1500 UTC. The daily precipitation is improved in the CPM with a spatial distribution similar to the GPM-IMERG precipitation and maximum rainfall located at the right place. As well as raising a large amount of dust, the haboob caused considerable damage along its route

Lithofacies and Sediment Sequences of a Microtidal, Wave-Dominated Tropical Estuary in Somone Lagoon (Senegal, West Africa)

Estuary sedimentary sequences have been the focus of several papers in the last decades; however, the majority these papers were centered in mesotidal and macrotidal estuaries of the middle latitudes. This present paper studies, from a sedimentological point of view, the infilling of a microtidal, wave-dominated tropical estuary, where wide tidal flats, mangroves and sabkhas are developed. Somone Lagoon is a Senegalese protected environment, very rich from an ecological point of view and with a definitive touristic vocation. For this work, 14 piston cores were studied. Additionally, the grain size, calcium carbonate and organic matter contents of 61 sediment samples vertically distributed in these cores were analyzed. The distribution of facies and the vertical sequences of sediments show the influence of the tropical seasonal fluctuations of fluvial sediment supply and evaporation processes. A high degree of bioturbation and an elevated organic content can be interpreted as the result of a high capacity of retention of the organic material into the estuary influenced by the weak tidal currents typical of a microtidal regime. These processes, acting since the last Holocene invasion of sea level, caused an advanced state of infilling of this estuarine system where both flood tidal deltas and bay head deltas prograde above the fine facies of the central domain of the estuary