Somatic embryogenesis from mature Bambusa ventricosa

This paper describes a bamboo tissue culture protocol based on explants from mature plants that minimizes the browning and rooting problems identified as bottlenecks in previous methods. The positive results of this initial research can be further optimized for commercial application

Mutation breeding of Phaseolus vulgaris L.: Studies on the effects of irradiation dosage to resolve a suitable procedure of handling M and M generations

Seedling growth studies on gamma-irradiated seeds of Phaseolus vulgaris were conducted in the greenhouse and the field. No significant differences in germination scores between the unirradiated control and the seeds exposed to 10, 20, 25, 30, 35 and 40 Krads of gamma radiation were obtained. Exposure to 100 Krads, however, was lethal to the apical meristem. Growth of the epicotyl under greenhouse conditions was the most affected by gamma radiation. Based upon a 30% reduction in epicotyl length the optimum dose for irradiation was 30 Krads. At this level of irradiation the survival score at harvest under field conditions was 50% of that of the unirradiated control. Treatment with 30 Krads also gave the highest number of solid chlorophyll mutants.While not all the chlorophyll mutants were scored in the Bs (Sl gle seed bulk) populations, the % occurrence was consistently higher than that for the corresponding Bp (single pod bulk) populations. Based on these results, it is recommended that: a) the most suitable level of gamma radiation to use in a mutation breeding programme is 30 Krads; b) the M1 seeds should be field-planted at double the planting density of the control; c) the M1 plants should be harvested such that the Bs (single seed bulk) population be used for screening of the mutants

Hybrid Amperometric and Potentiometric Sensing Based on a CMOS ISFET Array

Potentiometry and amperometry are some of the most important techniques for electroanalytical applications. Integrating these two techniques on a single chip using CMOS technology paves the way for more analysis and measurement of chemical solutions. In this paper, we describe the integration of electrode transducers (amperometry) on an ion imager based on an ISFET array (potentiometry). In particular, this integration enables the spatial representation of the potential distribution of active electrodes in a chemical solution under investigation

An Evaluation of the Use of Egg Yolk, Artemia nauplii, Microworms and Moina as Diets in Larval Rearing of Helostoma temmincki Cuvier and Valenciennes

Five day old Helostoma temmincki larvae measing 4.6 ± 0.1 mm in total length were stocked into 12 larval culture tanks at the rate of 1000 larvae/tank (l0 larvae/l). The larvae were fed Diet I (Egg yolk), Diet II (Egg yolk + Artemia naupliz), Diet III (Egg yolk +microworms), and Diet IV (Egg yolk + Moina) for iz period of4 weeks. After 1 week of rearing, larvae fed Diet III measun'ng 8. 4 mm total length and 7.0 mg weight were larger than larvae fed the other diets (P < 0.05). A t the end of two weeks, larvae fed Diets II, III and IV were larger than larvae fed Diet I (P < 0.05). FrOm" week 3 onwards, larvae fed Diet IV were consistently larger (P <0.05). This experiment shows that microworms were very suitable as a starter feed and that Moina was suitable for feeding Helostoma temmincki larvae after week 2 under tropical conditions

Monolithic Integration of a Plasmonic Sensor with CMOS Technology

Monolithic integration of nanophotonic sensors with CMOS detectors can transform the laboratory based nanophotonic sensors into practical devices with a range of applications in everyday life. In this work, by monolithically integrating an array of gold nanodiscs with the CMOS photodiode we have developed a compact and miniaturized nanophotonic sensor system having direct electrical read out. Doing so eliminates the need of expensive and bulky laboratory based optical spectrum analyzers used currently for measurements of nanophotonic sensor chips. The experimental optical sensitivity of the gold nanodiscs is measured to be 275 nm/RIU which translates to an electrical sensitivity of 5.4 V/RIU. This integration of nanophotonic sensors with the CMOS electronics has the potential to revolutionize personalized medical diagnostics similar to the way in which the CMOS technology has revolutionized the electronics industry

Online prostate cancer screening decision aid for at-risk men: A randomized trial

Objective: This study examines the efficacy of an online decision aid (DA) for men with a family history of prostate cancer. Methods: Unaffected Australian men (40 - 79 years) with at least one affected relative completed the first online questionnaire, were randomized to read either the tailored DA (intervention) or nontailored information about prostate cancer screening (control), then completed a questionnaire postreading and 12 months later. The primary outcome was decisional conflict regarding prostate specific antigen (PSA) testing. The impact of the DA on longitudinal outcomes was analyzed by using random intercept mixed effects models. Logistic and linear regressions were used to analyze the impact of the DA on screening behavior and decision regret. Stage of decision-making was tested as a moderator for decisional conflict and decision regret. The frequency of online material access was recorded. Results: the DA had no effect on decisional conflict, knowledge, inclination toward PSA testing, accuracy of perceived risk, or screening behavior. However, among men considering PSA testing, those who read the DA had lower decision regret compared with men who read the control materials, β=.34 , p \u3c.001, 95% confidence interval (CI) = [.22, .53]. Conclusions: This is the first study to our knowledge to evaluate the uptake and efficacy of an online screening DA among men with a family history of prostate cancer. Men who were undecided about screening at baseline benefitted from the DA, experiencing less regret 12 months later. In relation to decisional conflict, the control materials may have operated as a less complex and equally informative DA